Tuesday, August 15, 2017
Genetically Modified Vaccines Investigated
The Eugenics Investigation
(MonsantoInvestigation.com)
Michael James Ross
Published: August 15th, 2017
Updated: March 27th, 2023
Website: MonsantoInvestigation.com
PollutionScience.com
Chapter 1: Monsanto, Pfizer & Bayer
Chapter 2: Vaccine dangers
Chapter 3: HPV Vaccine (Gardasil)
Chapter 4: Pet & Animal Vaccines
Chapter 5: Genetically modified Vaccines & Viruses
Chapter 6: Nanotechnology & Biotechnology
Chapter 7: Cancer & Tumor Vaccines
Chapter 8: Lasers & Biophotonics
Chapter 9: Big Pharma
Chapter 10: Nagalase
Chapter 11: Miscellaneous
Chapter 12: Fetal tissues in Vaccines
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The following documents will detail evidence into the current issues with the production of harmful vaccines being manufactured by pharmaceutical companies. Additional information, documents and news articles can be found in the links below.
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Coronavirus Investigation News - Race Virus 201 - Pollution Science 101
March 15th, 2022
https://archive.org/details/covid-news_202302
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Coronavirus Investigation News – Race Virus 201 – Part 1
March 15th, 2022
https://pollutionscience101.wordpress.com/2023/02/16/coronavirus-investigation-news-race-virus-201/
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Coronavirus Investigation News – Race Virus 201 – Part 2
March 15th, 2022
https://pollutionscience101.wordpress.com/2023/02/16/coronavirus-investigation-news-race-virus-201-part-2/
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Coronavirus Investigation News – Race Virus 201 – Part 3
March 15th, 2022
https://pollutionscience101.wordpress.com/2023/02/16/coronavirus-investigation-news-race-virus-201-part-3/
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Coronavirus Investigation News – Race Virus 201 – Part 4
March 15th, 2022
https://pollutionscience101.wordpress.com/2023/02/16/coronavirus-investigation-news-race-virus-201-part-4/
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Coronavirus Investigation News – Race Virus 201 – Part 5
March 15th, 2022
https://pollutionscience101.wordpress.com/2023/02/16/coronavirus-investigation-news-race-virus-201-part-5/
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Coronavirus Investigation News – Race Virus 201 – Part 6
March 15th, 2022
https://pollutionscience101.wordpress.com/2023/02/16/coronavirus-investigation-news-race-virus-201-part-6/
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Coronavirus Investigation News – Race Virus 201 – Part 7
March 15th, 2022
https://pollutionscience101.wordpress.com/2023/02/16/coronavirus-investigation-news-race-virus-201-part-7/
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Coronavirus Investigation News – Race Virus 201 – Part 8
March 15th, 2022
https://pollutionscience101.wordpress.com/2023/02/16/coronavirus-investigation-news-race-virus-201-part-8/
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Coronavirus Investigation News – Race Virus 201 – Part 9
March 15th, 2022
https://pollutionscience101.wordpress.com/2023/02/16/coronavirus-investigation-news-race-virus-201-part-9/
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Coronavirus Investigation News – Race Virus 201 – Part 10
March 15th, 2022
https://pollutionscience101.wordpress.com/2023/02/16/coronavirus-investigation-news-race-virus-201-part-10/
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Coronavirus Investigation News – Race Virus 201 – Part 11
March 15th, 2022
https://pollutionscience101.wordpress.com/2023/02/16/coronavirus-investigation-news-race-virus-201-part-11/
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Coronavirus Investigation News – Race Virus 201 – Part 12
March 15th, 2022
https://pollutionscience101.wordpress.com/2023/02/16/coronavirus-investigation-news-race-virus-201-part-12/
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4/15/2020 - Coronavirus Investigation News - Race Virus 201 - Pollution Science 101 (Covid-19 & SARS-CoV-2)
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Race Virus 101 Part 1
https://pollutionscience101.wordpress.com/2023/03/25/race-virus-101-part-1/
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Race Virus 101 – Part 2
https://pollutionscience101.wordpress.com/2023/03/26/race-virus-101-part-2/
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Race Virus 101 – Part 3
https://pollutionscience101.wordpress.com/2023/03/26/race-virus-101-part-3/
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Race Virus 301 – Coronavirus Investigation News – Part 1
https://pollutionscience101.wordpress.com/2023/03/26/race-virus-301-coronavirus-investigation-news-part-1/
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Race Virus 301 – Coronavirus Investigation News – Part 2
https://pollutionscience101.wordpress.com/2023/03/26/race-virus-301-coronavirus-investigation-news-part-2/
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Pollution Science 101 - Book Archive (Web Archive)
https://archive.org/details/@pollution_science?tab=web-archive
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Pollution Science 101 - Video Archive
https://archive.org/details/@pollution_science
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Monsanto Investigation.com - Video Archive
https://archive.org/details/@monsantoinvestigation
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4/15/2020 - Coronavirus Investigation News - Race Virus 201 - Pollution Science 101 (COVID-19 & SARS-CoV-2)
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5/12/2019 - Race Virus 101 - The Eugenics Investigation (The Dysgenics Investigation) -
https://racevirus101.blogspot.com
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Chapter 1: Monsanto, Pfizer & Bayer
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The makers of a lethal-injection drug have become leaders in Arkansas' death-penalty battle
Apr. 17, 2017
Arkansas' plan to execute seven men by lethal injection over the course of 10 days in April hit a road block Friday when a federal judge moved to block all of the executions by issuing a restraining order against the state's use of the drug vecuronium bromide.
Arkansas was pushing to carry out the executions before one of the drugs used as part of the fatal cocktail expired.
Perhaps unexpectedly, the leading opponents of the executions were the very companies that produced the drugs.
Four companies have spoken out so far about the executions in Arkansas: Pfizer, Fresenius, West-Ward Pharmaceuticals, and the drug wholesaler McKesson.
There are three drugs typically used in the lethal-injection cocktail:
Midazolam, which is used as an anesthetic. In medicine, it's used to make people drowsy before surgery and as a way to produce memory loss so patients don't remember painful parts of the procedure. Midazolam is the drug set to expire in Arkansas, and its use has been controversial because of its role in recent botched executions in which patients remained conscious.
Vecuronium bromide, which causes paralysis. In medicine, it's used in general anesthesia to relax the skeletal muscles during surgery.
Potassium chloride, which is used to stop the heart. In medicine it's used to treat low blood potassium.
Over the past few years, drug companies have started blocking their drugs from being used in executions. These drugs also have other medical uses, which can make it tricky to keep them from making it into state prisons that still carry out the death penalty and to keep them from being used for that purpose.
In May, Pfizer, became the last pharmaceutical company to block the use of its drugs in lethal injections. Its decision meant there were no longer any Food and Drug Administration-approved manufacturers that would supply the drugs used in lethal injections for the death penalty.
"Pfizer strongly objects to the use of its products as lethal injections for capital punishment," the company said in its lethal-injection policy.
The move has made it harder for states to get the drugs for the purpose of lethal injection. Pfizer on Thursday said the drugs in Arkansas were sold to the state's Department of Correction without the company knowing:
http://www.businessinsider.com/arkansas-executions-drugs-using-lethal-injections-2017-4
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Former Monsanto is today known as Pharmacia LLC. Pharmacia is now a wholly owned subsidiary of Pfizer Inc.
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Pfizer VP Comes Clean & Tells The Truth About Pharmaceutical Companies (Video)
Below is a clip taken from the One More Girl documentary, a film that examines the Gardasil vaccine, which was designed to prevent Human Papillomavirus. In it, Dr. Peter Rost, MD, a former vice president of one of the largest pharmaceutical companies in the world (Pfizer), shares the truth about the ties between the medical and pharmaceutical industry.
http://www.collective-evolution.com/2016/05/14/pfizer-vp-comes-clean-tells-the-truth-about-pharmaceutical-companies-video/
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The Bayer corporation has recently bought and acquired the Monsanto Company for 66 billion dollars. Let us look at the past history of Bayer knowingly and willingly selling Hemophilia medication that was tainted with the HIV virus.
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Bayer Exposed ( HIV Contaminated Vaccine )
Nov 2006
https://www.youtube.com/watch?v=wg-52mHIjhs
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Contaminated haemophilia blood products
Contaminated haemophilia blood products were a serious public health problem in the late 1970s up to 1985.
These products caused large numbers of haemophiliacs to become infected with HIV and hepatitis C. The companies involved included Alpha Therapeutic Corporation, Institut Mérieux (which then became Rhone-Poulenc Rorer Inc., and is now part of Sanofi), Bayer Corporation and its Cutter Biological division, Baxter International and its Hyland Pharmaceutical division.[1] Estimates range from 6,000 to 10,000 haemophiliacs in the United States becoming infected with HIV.[1][2]
Factor VIII is a protein that helps the clotting of blood, which haemophiliacs, due to the genetic nature of their condition, are unable to produce themselves. By injecting themselves with it, hemophiliacs can stop bleeding or prevent bleeding from starting; some use it as often as three times a week.[
https://en.wikipedia.org/wiki/Contaminated_haemophilia_blood_products
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Monsanto pesticide found to infect plants with AIDS-like disease
May 18, 2011
(NaturalNews) When Dr. Don Huber, professor emeritus at Purdue University and internationally-recognized plant pathologist, wrote a letter back in January to US Department of Agriculture (USDA) Secretary Tom Vilsack, warning him about a new mystery disease showing up primarily in genetically-modified (GM) crops, the notice fell on deaf ears.
Research conducted by a team of senior plant and animal scientists found that Monsanto's glyphosate chemical, which is the primary ingredient in its popular RoundUp herbicide formula, appears responsible for infecting plants with an AIDS-like syndrome that destroys their immunity, blocks their absorption of certain vitamins and minerals, and eventually kills them.
Dr. Huber explains in his letter that the destruction caused by glyphosate does not appear limited to plants, either -- animals and humans alike that consume foods tainted with the chemical, or that are exposed in some other way to it, are potentially susceptible to developing the same autoimmune disorder. For this reason, Dr. Huber urged the USDA to further investigate RoundUp, as well as to deny the deregulation of GM alfalfa.
http://www.naturalnews.com/032441_pesticide_AIDS.html
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Flashback: Escaped, Mutated GM Maize on the Loose Maybe Carrying AIDS Virus
USDA Refused to Admit Which Pharmaceutical GM Crop Escaped Test Fields and Has Been On the Loose Since 2002!
Aaron Dykes
Infowars.com
April 24, 2012
Yesterday on the Alex Jones Show, Alex addressed genetically-modified, Big PHARMA crops carrying the AIDS virus in corn circa 2002. Clearly, biotech, the military, other entities have been dangerously experimenting on the public without consent for some time. But is something so out of control and unregulated as growing the AIDS virus really going on? Yes, shockingly, it’s true!
https://www.infowars.com/flashback-escaped-mutated-gm-maize-on-the-loose-maybe-carrying-aids-virus/
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GM plants will be used to create Aids vaccine; European Farmers Eager to Try Biotech; Farm Disaster in Zimbabwe
July 13, 2004
Genetically modified plants are to be used to grow vaccines against rabies and Aids, scientists have announced.
Europe's first field trial, announced yesterday, is likely to be carried out in South Africa because of fears over crop vandalism in Britain.
The GM crop could dramatically reduce the cost of producing vaccines � scientists estimate they can be made at between a tenth and a hundredth of the price of conventional immunisations.
http://www.agbioworld.org/newsletter_wm/index.php?caseid=archive&newsid=2192
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HIV Drug Grown in Genetically Engineered Plant Approved for Human Testing
By | July 20, 2011
Cocaine. Aspirin. Caffeine. Heroin. Ginseng. Nicotine. The list of powerful medications naturally produced by plants goes on and on. Unfortunately, no plant makes a drug capable of combating HIV. That is, no plant did until researchers at Germany's Fraunhofer Institute genetically engineered tobacco plants to produce specially designed antibodies that prevent transmission of the deadly virus.
Yesterday, the U.K. approved antibody therapy derived from Fraunhofer's genetically engineered tobacco for use on 11 human test subjects. The trial will test the safety of a plant-derived antibody designed to stop the transmission of HIV between sexual partners when applied directly to the vaginal cavity. This marks the first instance of such testing in Europe, where both governments and citizens remain largely skeptical of genetic modified products.
"This is a red letter day for the field," said Julian Ma, a Professor of Molecular Immunology at St George's, University of London, and researcher on the tobacco project. "The approval from the MHRA for us to proceed with human trials is an acknowledgement that monoclonal antibodies can be made in plants to the same quality as those made using existing conventional production systems. That is something many people did not believe could not be achieved."
https://www.livescience.com/15146-hiv-medication-fraunhofer-tobacco-genetic-engineering.html
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Pfizer
https://en.wikipedia.org/wiki/Pfizer#GMO_virus
Pfizer Inc. /ˈfaɪzər/ is an American pharmaceutical corporation headquartered in New York City,[4] with its research headquarters in Groton, Connecticut. It is among the world's largest pharmaceutical companies.[5] It is listed on the New York Stock Exchange, and its shares have been a component of the Dow Jones Industrial Average since 2004.[6]
Pfizer develops and produces medicines and vaccines for a wide range of medical disciplines, including immunology, oncology, cardiology, diabetology/endocrinology, and neurology. Its products include the blockbuster drug Lipitor (atorvastatin), used to lower LDL blood cholesterol; Lyrica (pregabalin) for neuropathic pain/fibromyalgia; Diflucan (fluconazole), an oral antifungal medication; Zithromax (azithromycin), an antibiotic; Viagra (sildenafil) for erectile dysfunction; and Celebrex/Celebra (celecoxib), an anti-inflammatory drug.
Litigation
Pfizer is party to a number of lawsuits stemming from its pharmaceutical products as well as the practices of various companies it has merged with or acquired.[114][115][116]Quigley Co.
Pfizer acquired Quigley in 1968, and the division sold asbestos-containing insulation products until the early 1970s.[125] Asbestos victims and Pfizer have been negotiating a settlement deal that calls for Pfizer to pay $430 million to 80 percent of existing plaintiffs. It will also place an additional $535 million into an asbestos settlement trust that will compensate future plaintiffs as well as the remaining 20 percent of current plaintiffs with claims against Pfizer and Quigley. The compensation deal is worth $965 million all up. Of that $535 million, $405 million is in a 40-year note from Pfizer, while $100 million will come from insurance policies.Bjork–Shiley heart valve
Pfizer purchased Shiley in 1979 at the onset of its Convexo-Concave valve ordeal, involving the Bjork–Shiley heart valve. Approximately 500 people died when defective valves failed and, in 1994, the United States ruled against Pfizer for ~$200 million.[126][127]Abdullahi v. Pfizer, Inc.
In 1996, an outbreak of measles, cholera, and bacterial meningitis occurred in Nigeria. Pfizer representatives traveled to Kano, Nigeria to administer an experimental antibiotic, trovafloxacin, to approximately 200 children. Local Kano officials report that more than 50 children died in the experiment, while many others developed mental and physical deformities.[128] The nature and frequency of both fatalities and other adverse outcomes were similar to those historically found among pediatric patients treated for meningitis in sub-Saharan Africa.[129] In 2001, families of the children, as well as the governments of Kano and Nigeria, filed lawsuits regarding the treatment.[130] According to the news program Democracy Now!, "[r]esearchers did not obtain signed consent forms, and medical personnel said Pfizer did not tell parents their children were getting the experimental drug."[131] The lawsuits also accuse Pfizer of using the outbreak to perform unapproved human testing, as well as allegedly under-dosing a control group being treated with traditional antibiotics in order to skew the results of the trial in favor of Trovan. While the specific facts of the case remain in dispute, both Nigerian medical personnel and at least one Pfizer physician have stated that the trial was conducted without regulatory approval.[132][133]In 2007, Pfizer published a Statement of Defense letter.[134] The letter states that the drug's oral form was safer and easier to administer, that Trovan had been used safely in over 5000 Americans prior to the Nigerian trial, that mortality in the patients treated by Pfizer was lower than that observed historically in African meningitis epidemics, and that no unusual side effects, unrelated to meningitis, were observed after 4 weeks.
In June 2010, the US Supreme Court rejected Pfizer's appeal against a ruling allowing lawsuits by the Nigerian families to proceed.[135]
In December 2010, WikiLeaks released US diplomatic cables, which indicate that Pfizer had hired investigators to find evidence of corruption against Nigerian attorney general Aondoakaa to persuade him to drop legal action.[136] Washington Post reporter Joe Stephens, who helped break the story in 2000, called these actions "dangerously close to blackmail."[131] In response, the company has released a press statement describing the allegations as "preposterous" and stating that they acted in good faith.[137] Aondoakka, who had allegedly demanded bribes from Pfizer in return for a settlement of the case,[138] was declared unfit for office and had his U.S. visa revoked in association with corruption charges in 2010.
GMO virus
A scientist claims she was infected by a genetically modified virus while working for Pfizer. In her federal lawsuit she says she has been intermittently paralyzed by the Pfizer-designed virus. "McClain, of Deep River, suspects she was inadvertently exposed, through work by a former Pfizer colleague in 2002 or 2003, to an engineered form of the lentivirus, a virus similar to the one that can lead to acquired immune deficiency syndrome, also known as AIDS."[141] The court found that McClain failed to demonstrate that her illness was caused by exposure to the lentivirus,[142] but also that Pfizer violated whistleblower laws.Pharmacia acquisition
In 2002, Pfizer merged with Pharmacia. The merger was again driven in part by the desire to acquire full rights to a product, this time Celebrex (celecoxib), the COX-2 selective inhibitor previously jointly marketed by Searle (acquired by Pharmacia) and Pfizer. In the ensuing years, Pfizer carried out a massive restructuring that resulted in numerous site closures and the loss of jobs including Terre Haute, Indiana; Holland, Michigan; Groton, Connecticut; Brooklyn, New York; Sandwich, UK; and Puerto Rico.Pharmacia had been formed by a series of mergers and acquisitions from its predecessors, including Searle, Upjohn and SUGEN.
Searle was founded in Omaha, Nebraska, in April 1888. The founder was Gideon Daniel Searle. In 1908, the company was incorporated in Chicago, Illinois. In 1941, the company established headquarters in Skokie, Illinois. It was acquired by the Monsanto Company, headquartered in St. Louis, Missouri, in 1985.
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The Cutter Incident: How America's First Polio Vaccine Led to a Growing Vaccine Crisis
2006
In April 1955 more than 200 000 children in five Western and mid-Western USA states received a polio vaccine in which the process of inactivating the live virus proved to be defective. Within days there were reports of paralysis and within a month the first mass vaccination programme against polio had to be abandoned. Subsequent investigations revealed that the vaccine, manufactured by the California-based family firm of Cutter Laboratories, had caused 40 000 cases of polio, leaving 200 children with varying degrees of paralysis and killing 10.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1383764/
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Cutter Laboratories
Other incidents
In the 1980s, numerous companies, including Bayer's Cutter Biologic division, produced unsafe blood products to treat hemophilia. The pharmaceutical product—produced from blood from donors across the US—was contaminated with the HIV virus at a time when HIV could not be screened out. These problems led to lawsuits over the next twenty years.[9]
A recent German documentary called "Tödlicher Ausverkauf: Wie BAYER AIDS nach Asien importierte" (Deadly Sale: How Bayer imported AIDS into Asia) researched the Koate product for hemophiliacs sold by Bayer's Cutter division under full knowledge of its HIV contamination. Cutter ex-manager Merill Boyce expressed the opinion that the company should be responsible and pay damages. Another ex-manager John H Hink, who also had been on the team that marketed Koate to Asia, expressed regret in the documentary that management had required that they sell old stock despite knowledge of HIV contamination. Lexi J Hazan and Charles A Kozak are attorneys who represent victims against Bayer AG in the Koate cases. Thomas C Drees is a consultant that researched the Koate Cutter case.
The Cutter incident
On April 12, 1955, following the announcement of the success of polio vaccine trial, Cutter Laboratories became one of several companies that was recommended to be given a licence by the United States government to produce Salk's polio vaccine. In anticipation of the demand for vaccine, the companies had already produced stocks of the vaccine and these were issued once the licences were signed.
In what became known as the Cutter incident, some lots of the Cutter vaccine—despite passing required safety tests—contained live polio virus in what was supposed to be an inactivated-virus vaccine. Cutter withdrew its vaccine from the market on April 27 after vaccine-associated cases were reported.
The mistake produced 120,000 doses of polio vaccine that contained live polio virus. Of children who received the vaccine, 40,000 developed abortive poliomyelitis (a form of the disease that does not involve the central nervous system), 56 developed paralytic poliomyelitis—and of these, five children died from polio.[2] The exposures led to an epidemic of polio in the families and communities of the affected children, resulting in a further 113 people paralyzed and 5 deaths.[3] The director of the microbiology institute lost his job, as did the equivalent of the assistant secretary for health. Secretary of Health, Education, and Welfare Oveta Culp Hobby stepped down. Dr Sebrell, the director of the NIH, resigned.[4]
Surgeon General Scheele sent Drs. William Tripp and Karl Habel from the NIH to inspect Cutter's Berkeley facilities, question workers, and examine records. After a thorough investigation, they found nothing wrong with Cutter's production methods.[5] A congressional hearing in June 1955 concluded that the problem was primarily the lack of scrutiny from the NIH Laboratory of Biologics Control (and its excessive trust in the National Foundation for Infantile Paralysis reports).[4]
A number of civil lawsuits were filed against Cutter Laboratories in subsequent years, the first of which was Gottsdanker v. Cutter Laboratories.[6] The jury found Cutter not negligent, but liable for breach of implied warranty, and awarded the plaintiffs monetary damages. This set a precedent for later lawsuits. All five companies that produced the Salk vaccine in 1955—Eli Lilly, Parke-Davis, Wyeth, Pitman-Moore, and Cutter—had difficulty completely inactivating the polio virus. Three companies other than Cutter were sued, but the cases settled out of court.[7]
The Cutter incident was one of the worst pharmaceutical disasters in U.S. history, and exposed several thousand children to live polio virus on vaccination.[3] The NIH Laboratory of Biologics Control, which had certified the Cutter polio vaccine, had received advance warnings of problems: in 1954, staff member Dr. Bernice Eddy had reported to her superiors that some inoculated monkeys had become paralyzed (pictures were sent as well). William Sebrell, the director of NIH wouldn't hear of such a thing
https://en.wikipedia.org/wiki/Cutter_Laboratories
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IG Farben
IG Farben was a German chemical and pharmaceutical industry conglomerate. Its name is taken from Interessen-Gemeinschaft Farbenindustrie AktienGesellschaft (Plc Syndicate [literally, "community of interests"] of dye-making corporations). The company was formed in 1925 from a number of major chemical companies that had been working together closely since World War I. During its heyday, IG Farben was both the largest company in Europe overall and the largest chemical and pharmaceutical company in the world.
Founding members
IG Farben was founded on 9 December 1925, as a merger of the following six companies:[3]
- BASF
- Bayer
- Hoechst (including Cassella and Chemische Fabrik Kalle)
- Agfa
- Chemische Fabrik Griesheim-Elektron
- Chemische Fabrik vorm. Weiler Ter Meer
In 1941, an investigation exposed a "marriage" cartel between John D. Rockefeller's United States-based Standard Oil Co. and I.G. Farben.[16][17][18][19] It also brought new evidence concerning complex price and marketing agreements between DuPont,[citation needed] a major investor in and producer of leaded gasoline, United States Industrial Alcohol Company and its subsidiary, Cuba Distilling Co. The investigation was eventually dropped, like dozens of others in many different kinds of industries, due to the need to enlist industry support in the war effort.[citation needed] However, the top directors of many oil companies agreed to resign, and oil industry stocks in molasses companies were sold off as part of a compromise worked out.[20][21][22]
IG Farben had bought the patent for the pesticide Zyklon B, which had been invented by the Nobel Prize-winning Jewish German chemist Fritz Haber's research group at the Institute for Physical Chemistry and Electrochemistry in the 1920s, and which was originally used as an insecticide, especially as a fumigant in grain stores. IG Farben licensed the pesticide to various companies, including the American Cyanamid Company for use, for example, in de-lousing incoming Mexican immigrants in the 1930s, and to the German company Degesch (Deutsche Gesellschaft für Schädlingsbekämpfung), founded by Fritz Haber, and whose products were used in Holocaust gas chambers. IG Farben owned 42.2 % of the shares of Degesch and was represented in its supervisory board. Pesticides similar to Zyklon B remain in production by other companies, and are used e.g. as insecticides.
Of the 24 directors of IG Farben indicted in the so-called IG Farben Trial (1947–1948) before a U.S. military tribunal at the subsequent Nuremberg Trials, 13 were sentenced to prison terms between one and eight years, but most were quickly released and several became senior industry executives in the post-war companies that split off from IG Farben and other companies.
https://en.wikipedia.org/wiki/IG_Farben
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Rockefeller Foundation Developed Vaccines For “Mass-Scale” Fertility Reduction
August 5, 2010
In its 1968 yearly report, the Rockefeller Foundation acknowledged funding the development of so-called “anti-fertility vaccines” and their implementation on a mass-scale. From page 51 onward we read:
“(…) several types of drugs are known to diminish male fertility, but those that have been tested have serious problems of toxicity. Very little work is in progress on immunological methods, such as vaccines, to reduce fertility, and much more research is required if a solution is to be found here.”
The possibility of using vaccines to reduce male fertility was something that needed to be investigated further, according to the Rockefeller Foundation, because both the oral pill and the IUD were not suitable for mass-scale distribution:
“We are faced with the danger that within a few years these two “modern” methods, for which such high hopes have been held, will in fact turn out to be impracticable on a mass scale.”
The possibility of administrating hormone preparations to reduce fertility was also mentioned, although- states the report- they have been known to “cause bleeding problems, which may limit their usefulness.”
“A semipermanent or renewable subcutaneous implant of these hormones has been suggested, but whether or not the same difficulties would result has not been determined.”
Saying that research thus-far had been too low-grade to produce any substantial results, the report was adamant:
“The Foundation will endeavour to assist in filling this important gap in several ways:
https://www.infowars.com/rockefeller-foundation-developed-vaccines-for-%E2%80%9Cmass-scale%E2%80%9D-fertility-reduction/
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Merck Scientists Expose Massive Vaccine Fraud
Jul 6, 2012
https://www.youtube.com/watch?v=8qZFgYBDIpM
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Shingles Vaccine Dangers Exposed In FDA Letter to Merck
May 1st 2017
The CDC claims the shingles vaccine is safe, but a letter the FDA wote to Merck paints a different picture.
http://www.greenmedinfo.com/blog/shingles-vaccine-dangers-exposed-fda-letter-merck
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Merck Group
In September 2014 Merck halted the clinical development of two drug candidates in development with Oxygen Biotherapeutics. One drug candidate suffered a lack of success in patient recruitment, with its MUC1 antigen-specific cancer immunotherapy drug, tecemotide (L-BLP25), missing its Phase I/II endpoint of increasing overall survival in patients with Stage III non-small cell lung cancer.[19] Later in September it was announced that the company would acquire Sigma-Aldrich for $17 billion.[20] After the acquisition was completed in 2015, Merck has around 50,000 employees.[3] In November 2014, Merck and Pfizer agreed a deal for the latter to sell the former sharing rights to develop an experimental immunotherapy drug for a fee of $850 million.[21]
In October 2015, Merck revealed it would return the rights of the Kuvan drug to BioMarin Pharmaceutical Inc.. The drug is effective in the treatment of the rare genetic disorder PKU, however Merck is moving away from genetics into cancer treatment, immunology and neurology.[22] In October, it was announced that Karl-Ludwig Kley, CEO of Merck since April 2007, would retire in April 2016, and be succeeded by Stefan Oschmann, who has been a member of the executive board since 2011
The company started building new facilities for its R&D operations and a new visitor center at its Darmstadt site in the early 2010s by 2015 had torn down the site's pyramid as part of the renovations.[24]
In April 2017, the company announced it had completed the acquisition of food safety testing company, BioControl Systems Inc.
https://en.wikipedia.org/wiki/Merck_Group
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Merck developer admits vaccines contain hidden cancer viruses derived from diseased monkeys
September 8, 2013
If you haven’t yet realized the truth about how vaccines contain hidden cancer viruses, prepare yourself to be shocked by the admission you’re about to hear. Decades ago, one of the most prominent vaccine scientists in the history of the vaccine industry — a Merck scientist — made a recording where he openly admitted that vaccines given to Americans were contaminated with leukemia and cancer viruses.
In hearing this admission, his colleagues (who are also recorded here) break into laughter and seem to think it’s hilarious. They then suggest that because these vaccines are first tested in Russia, their side effects will help the U.S. win the Olympics because the Russian athletes will all be “loaded down with tumors.”
For the record, this is the same vaccine that was given to tens of millions of Americans and promoted by the government. To this day, people still carry these hidden cancer viruses which have proven to be a boon to the cancer industry.
Why vaccine scientists lie to the public
The presence of SV40 cancer viruses in vaccines isn’t some conspiracy theory, by the way: these are the words of a top Merck scientist who probably had no idea that his recording would be widely heard across the internet one day. He probably thought this conversation would remain a secret forever. When asked why this didn’t get out to the press, he replied “Obviously you don’t go out, this is a scientific affair within the scientific community.”
In other words, vaccine scientists cover for vaccine scientists. They keep all their dirty secrets within their own circle of silence and don’t reveal the truth about the contamination of their vaccines.
https://www.infowars.com/merck-developer-admits-vaccines-contain-hidden-cancer-viruses-derived-
from-diseased-monkeys/
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Chapter 2: Vaccine dangers
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CDC Destroyed Evidence That Some Vaccines Cause Autism
Government agency caught tricking the American public
August 11, 2017
https://www.infowars.com/cdc-destroyed-evidence-that-some-vaccines-cause-autism/
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CDC Sends Fact Sheet Linking Polio Vaccine to Cancer Down the Memory Hole
July 19, 2013
https://www.infowars.com/cdc-sends-fact-sheet-linking-polio-vaccine-to-cancer-down-the-memory-hole/
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Shock: Scientists Work To Destroy Old Polio Vaccine After Admitting it Causes Polio
“Some of that virus could leak out into the world, and we could have outbreaks of a type of polio we haven’t seen since 1999″
April 19, 2016
https://www.infowars.com/shock-scientists-work-to-destroy-old-polio-vaccine-after-admitting-it-causes-polio/
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MMR vaccine isn't safe after all, UK government forced to concede
September 1st 2010
After years of reassuring parents about the safety of the MMR (measles, mumps, rubella) vaccine, the UK government has finally had to concede that it can have serious side effects. A UK court has ruled that the vaccine caused severe brain damage in a boy, now aged 18, and has ordered the government to pay compensation. The ruling brings to an end a lengthy campaign by the boy's mother, Jackie Fletcher, who created the pressure group JABS to help other parents win compensation for their vaccine-damaged children. Jackie's son, Robert, was just 13 months old when he had the MMR vaccination. From being a healthy baby who was developing normally, he started to suffer epileptic fits and became unresponsive. He is now severely disabled. Jackie and her family have been awarded lb91,000 in compensation. The judgement also sends out hope to the thousands of parents in the UK who are also fighting for compensation. (Source: Sunday Times, August 29, 2010).
https://www.wddty.com/news/2010/09/mmr-vaccine-isnt-safe-after-all-uk-government-forced-to-concede.html?
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"Direct Order" Documentary (Full) - Soldiers Ordered To Take Anthrax Vaccine & Got Brain Damaged
Sep 8, 2013
https://www.youtube.com/watch?v=wDDMsvErsQw
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New JAMA Study Confirms Nurse Whistleblower: Routine Hospital Vaccine Damage Happening to Infants
Journal of the American Medical Association study confirms whistleblower’s testimony that hospitals are covering up infant vaccine injury
June 22, 2015
https://www.infowars.com/new-jama-study-confirms-nurse-whistleblower-routine-hospital-vaccine-damage-happening-to-infants/
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U.S. Media Blackout: Italian Courts Rule Vaccines Cause Autism
February 10, 2015
https://www.infowars.com/u-s-media-blackout-italian-courts-rule-vaccines-cause-autism/
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Study: Infants With DTP Vaccine 5 to 10-Times More Susceptible to Dying
Infants with vaccine at greater risk of dying from other causes
April 24, 2017
https://www.infowars.com/study-infants-with-dtp-vaccine-5-to-10-times-more-susceptible-to-dying/
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Infant mortality rates regressed against number of vaccine doses routinely given: Is there a biochemical or synergistic toxicity?
2011
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170075/
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UFC Fighter Blames DTaP on Son’s Sudden Death…Sounds Off On ALL VACCINES
http://vaxxter.com/ufc-fighter-blames-dtap-on-sons-sudden-death/
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The Risks of the Whooping Cough Vaccine
Oct 9, 2010
https://www.youtube.com/watch?v=Qj4VjENIe8A
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15 children die in botched measles vaccine campaign
South Sudan’s government is now saying that
15 children have died at the hands of a botched measles vaccine
campaign. All of the deaths were children under the age of five.
Sudan’s health ministry is blaming two factors for the mass deaths.
They used the same syringe for all children.
Mealses vaccines were not properly stored.
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INVESTIGATION: Over 200 People Suffer Flu Shot Injuries
12/20/2016
http://vaxxter.com/investigation-over-200-people-suffer-flu-shot-injuries/
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Breaking: Interview with Vaxxed Producer who was Banned from Australia
Doctors, medical bureaucrats, and government officials in Australia are foaming at the mouth
August 9, 2017
Polly Tommey, producer of the famous documentary, Vaxxed (trailer), has been banned from Australia. If that sounds quite insane—it is.
Vaxxed has been screening across the world. It is an explosive revelation about egregious fraud at the US Centers for Disease Control (CDC).
The film focuses on the 2014 public confession of a long-time researcher at the CDC, William Thompson. Thompson admits that he and his colleagues committed a crime, by manipulating data to give the MMR vaccine a free pass, “proving” it had no connection to autism—when in fact, as Thompson states, the vaccine does raise the risk of autism in children.
https://www.infowars.com/breaking-interview-with-vaxxed-producer-who-was-banned-from-australia/
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Vaccines Exposed: The Hidden Crime Against Children
Boston Herald says people should be hanged for being vaccine choice
May 20, 2017
https://www.infowars.com/vaccines-exposed-the-hidden-crime-against-children/
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Kennedy, De Niro Hold Major Vaccine Safety Press Conference
Prepared to offer unique challenge to American people, media
February 15, 2017
https://www.infowars.com/kennedy-de-niro-to-hold-major-vaccine-safety-press-conference/
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Nephew of JFK: Vaccines are a “Holocaust”
Bill to make shots mandatory in California gets first hearing
April 8, 2015
https://www.infowars.com/nephew-of-jfk-vaccines-are-a-holocaust/
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Trump Opposed Vaccines, Warned of Autism
Believes Autism Spectrum Disorders (ASD) are linked to exposure to vaccines
August 9, 2015
https://www.infowars.com/trump-opposed-vaccines-warned-of-autism/
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Rand Paul on Vaccines: I’m All for Them, ‘but I’m also for freedom’
Upholds the right of parents to mete out vaccines as they see fit
September 17, 2015
https://www.infowars.com/rand-paul-weighs-in-on-vaccines-informed-consent/
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Nearly two dozen medical studies prove that vaccines can cause autism
October 1, 2013
https://www.infowars.com/nearly-two-dozen-medical-studies-prove-that-vaccines-can-cause-autism/
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22 Medical Studies That Show Vaccines Can Cause Autism
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Study finds association between childhood vaccination and the onset of neuropsychiatric disorders
Aug 5, 2017
http://www.naturalhealth365.com/category/vaccine-dangers/
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Bombshell: CDC Commits new Vaccine-autism Crime
CDC won’t allow its own whistleblower to testify in vaccine-damage case
October 21, 2016
Here are the bones of the story. For the first time in 30 years, a vaccine-damage case has gone before a court judge. Lawyers for a 16-year-old autistic boy are suing a medical clinic for administering vaccines that brought about the autism. The CDC, of course, denies any connection between vaccines and autism. But one of its own long-time researchers, William Thompson, has publicly confessed to fraud in that area. Thompson states that he and his colleagues concealed research data that would have shown the MMR vaccine and mercury-laden vaccines do cause autism. The lawyers for the 16-year-old boy want to bring in Thompson to testify about what he knows. The CDC has said NO. The head of the CDC, Thomas Frieden, states, “Dr. William Thompson’s deposition testimony would not substantially promote the objectives of CDC or HHS [the Department of Health and Human Services].”
Well, he’s right, because the CDC is the PR arm of the vaccine industry. The CDC is a major purchaser of vaccines for the US federal government. If this boy won his case, other cases would follow. The potential monetary exposure in judgments? A trillion dollars or more.
Ecowatch.com has the details:
https://www.infowars.com/bombshell-cdc-commits-new-vaccine-autism-crime/
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Mercury in vaccines: “No more dangerous than the mercury in a tuna sandwich”
March 10, 2010
Over the last few years, the American people have become increasingly aware of the dangers of vaccination. This is due, in large part, to those in the independent media that had the courage to report on the serious side effects of vaccine ingredients such as aluminum, polysorbate 80, thimerosal, and squalene. As a result of increased education regarding vaccines, many Americans have come to resist the toxic injections. Indeed, nowhere was this more evident than the recent swine flu scam that left thousands and thousands of unused vaccine vials stuck on the shelves of pharmacies and health departments all across the country. Yet the vaccine and pharmaceutical companies, many medical doctors, and government health agencies have engaged in a massive public relations scheme in order to convince the population to forsake real science as well as their own common sense and take the shot. During the swine flu scare America was subjected to the usual scare tactics of medical doctors, intense media hype and alarm, and even ridiculous statements claiming that the mercury contained in vaccines is no more dangerous than the mercury in a tuna sandwich. (Infowars) This latter statement is of particular interest due to the fact that it is similar to some of the arguments vaccinators have used for some time and continue to use in their efforts to cast doubt upon the harmful effects of thimerosal. Mixing truth with fiction, they claim that ethylmercury (the mercury used in vaccines) is a very different form of mercury than methylmercury (the mercury found in fish) and that, between the two, methylmercury is more dangerous. A closer examination of the argument however, reveals a much different conclusion.
First, one must understand a few facts regarding thimerosal. Used as a preservative, thimerosal is made up of 49.6% mercury, a known neurotoxin. (Immunizationinfo.org) There are 25 mcg of mercury included in each regular season flu shot while the EPA sets the toxicity limit of mercury at .1 mcg. This is a 250 times greater amount than the EPA considers the toxic level in each injection. (Safeminds) While denial by vaccine makers and welfare scientists may exist as to the effects of ethylmercury compared to methylmercury, there can be no denial that mercury, in any form, is harmful to the brain. The argument made by vaccinators, of course, is that the harmful effects of ethylmercury are negligible when weighed against the benefits of the vaccine.
https://www.infowars.com/mercury-in-vaccines-no-more-dangerous-than-the-mercury-in-a-tuna-sandwich/
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Neuroscientist Makes It Clear Why Aluminum Adjuvants Should Not Be In Vaccines
http://www.collective-evolution.com/2015/01/29/neuroscientist-makes-it-clear-why-aluminum-adjuvants-should-not-be-in-vaccines/
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Dispelling Myths Regarding The Use of Thimerosal in Vaccines
Numerous news outlets promote vaccines as universally safe
July 11, 2017
https://www.infowars.com/dispelling-myths-regarding-the-use-of-thimerosal-in-vaccines/
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Vaccines are not associated with autism: An evidence-based meta-analysis of case-control and cohort studies
17 June 2014
http://www.sciencedirect.com/science/article/pii/S0264410X14006367
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Some scientists disagree with studies that claim vaccines do not cause autism.
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How the US government admits vaccines cause autism
What? The government admits vaccines cause autism?
September 8, 2014
https://www.infowars.com/how-the-us-government-admits-vaccines-cause-autism/
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CDC Scientist Admits Mercury in Vaccines Cause Autism
Whistleblower continued to expose CDC cover up
September 8, 2014
https://www.infowars.com/cdc-scientist-admits-mercury-in-vaccines-cause-autism/
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Bang: Texas Prosecutor Video: “Vaccines cause autism”
As La Hood talks, the vaccine criminals and liars crumble into dust
August 31, 2016
https://www.infowars.com/bang-texas-prosecutor-video-vaccines-cause-autism/
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Impairment of mitochondrial energy metabolism in different regions of rat brain following chronic exposure to aluminium
2008
http://www.sciencedirect.com/science/article/pii/S0006899308017150
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Aluminum Toxicity Is Associated with Mitochondrial Dysfunction and the Production of Reactive Oxygen Species in Plant Cells
2002
http://www.plantphysiol.org/content/128/1/63
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Aluminum-Induced Mitochondrial Dysfunction Leads to Lipid Accumulation in Human Hepatocytes: A Link to Obesity
2007
https://www.karger.com/Article/Abstract/107546
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CDC Study Shows Up to 7.7-fold Greater Odds of Miscarriage After Influenza Vaccine
September 19, 2017
https://childrenshealthdefense.org/news/cdc-study-shows-7-7-fold-greater-odds-miscarriage-influenza-vaccine/
The CDC has just published a seismic study (Donahue et al.) linking spontaneous abortions in women to flu vaccines. The study reviewed data for the 2010-11 and 2011-12 flu seasons. Women vaccinated with the inactivated influenza vaccine (IIV) in the 2010-2011 season had 3.7-fold greater odds of experiencing a spontaneous abortion within 28 days than women not receiving the vaccine. Over the entire study period (2010 to 2012), the odds for a spontaneous abortion for vaccinated women were 2.0 times greater than for those women not receiving the flu vaccine. Both figures showed a statistically significant increase in miscarriages when women received their flu shot. The median gestational age for spontaneous abortion was 7 weeks in the affected pregnancies. Most alarmingly, in women who received the H1N1 vaccine in the previous flu season, the odds of spontaneous abortion in the 28 days after receiving a flu vaccine were 7.7 times greater. For every flu season, starting in 2010-2011, there has been an H1N1-type virus included in regular flu shots in the United States. For the current 2017-18 flu season, the CDC still recommends that all flu vaccines contain H1N1. This study raises concerns whether this particular strain of influenza antigen is safe during pregnancy.
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Increased Risk of Noninfluenza Respiratory Virus Infections Associated With Receipt of Inactivated Influenza Vaccine
March 15, 2012
https://academic.oup.com/cid/article/54/12/1778/455098
Abstract
We randomized 115 children to trivalent inactivated influenza vaccine (TIV) or placebo. Over the following 9 months, TIV recipients had an increased risk of virologically-confirmed non-influenza infections (relative risk: 4.40; 95% confidence interval: 1.31-14.8). Being protected against influenza, TIV recipients may lack temporary non-specific immunity that protected against other respiratory viruses.
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DR Oz: ‘The vaccine increases your chances of getting Covid-19’
September 21, 2020
https://twitter.com/BeyondLounger/status/1307977962294325249
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Human trials of Oxford coronavirus vaccine paused over ‘spinal-cord disease fears’
Sep 21, 2020
Leaked report says volunteer in UK was left unable to walk after receiving two doses
https://www.theweek.co.uk/108143/human-trials-oxford-coronavirus-vaccine-paused-spinal-cord-disease-fears
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CDC’s Own Expert Vaccine Court Witness Confirmed Vaccines Can Cause Autism, So They Fired Him Immediately
January 8, 2019
https://www.activistpost.com/2019/01/cdcs-own-expert-vaccine-court-witness-confirmed-vaccines-can-cause-autism-so-they-fired-him-immediately.html---
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Is Alarming Rise in Autism Linked to 1988 Event?
November 15, 2011
- The first conjugate vaccine, against Haemophilus influenzae type b (Hib), was approved for use in the U.S. in 1988, which coincides with a marked increase in reported prevalence of autism spectrum disorders among American children
- Researchers hypothesize that the Hib vaccine may be in part responsible for this rise in autism, as conjugate vaccines appear to have a profound impact on neural development. These vaccines “fundamentally change the manner in which the immune systems of infants and young children function,” the authors of a recent study say
http://articles.mercola.com/sites/articles/archive/2011/11/15/vaccines-behind-autism-epidemic.aspx
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Infant Vaccines Produce Autism Symptoms in New Primate Study by University of Pittsburgh Scientists
Routine Safety Study That Government Scientists Refused To Do
Illustrates Vaccine Program And Mercury Health Risks Says SafeMinds
May 19, 2008Illustrates Vaccine Program And Mercury Health Risks Says SafeMinds
http://www.prnewswire.com/news-releases/infant-vaccines-produce-autism-symptoms-in-new-primate-study-by-university-of-pittsburgh-scientists-57263092.html
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The CDC's Role in Undoing Vaccine Exemptions: the NACCHO Front Group
March 8th 2015
http://www.greenmedinfo.com/blog/cdcs-role-undoing-vaccine-exemptions-naccho-front-group-1
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Vaccine Deaths And Injuries Skyrocket As Cover-Up Implodes
August 30, 2010
https://www.infowars.com/vaccine-deaths-and-injuries-skyrocket-as-cover-up-implodes/
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Supreme Court Upholds Liability Shield For Vaccine Makers
February 22, 2011
https://www.npr.org/sections/health-shots/2011/02/22/133964322/supreme-court-upholds-liability-shield-for-vaccine-makers
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Vaccination Myths and Truths
August 3, 2009
Suspicious H1N1’s Origins
On April 24, AP reported that
“Health officials are investigating a never-before-seen form of the flu that combines pig, bird and human viruses…. (It’s) a growing medical mystery because it’s unclear how (affected people) caught the virus. None (of seven cited had) contact with pigs.” Nor had others reportedly affected in other US cities.The “intercontinental” mixture included North American Swine Flu, North American Avian Flu, human H1N1 flu, and a fourth H3N2 strain found in Asia and Europe.
Suspicions about a synthetic laboratory-made virus have surfaced. Writing in NewsMax.com, Dr. Russell Blaylock quoted an unnamed viroligist saying: “Where the hell it got all these genes we don’t know.” According to Blalock: “Debate continues over the possibility that swine flu is a genetically engineered virus.”
- The possibility of a diabolical depopulation scheme can’t be dismissed. The idea’s been around for decades, including from the 1974 Henry Kissinger project – National Security Study Memorandum 200 (NSSM 200). It was backed by powerful economic interests to cull the world population of useless feeders so corporate giants could exploit world resources unimpeded.
Kissinger’s scheme was to make birth control a prerequisite for US aid. He wanted the annual death rate doubled and for a population decline in the hundreds of millions by 2000. Poor third world women in countries like Brazil were involuntarily sterilized. Millions were harmed then. Perhaps today’s toll from mandatory global Swine Flu vaccinations will be billions.
Yet US laws were passed to prevent it. In 1986, the National Childhood Vaccine Injury Act (NCVIA) required:
— giving parents written information on vaccine benefits and risks so they could decide on what was safe for their children;
— maintaining a permanent record of all vaccines given children, including producer names and lot numbers;
— keeping up to date medical records of all vaccinations given children; and
— recording all serious health problems after vaccinations were administered and notifying the federal Vaccine Adverse Event Reporting System (VAERS) immediately.
https://www.infowars.com/vaccination-myths-and-truths/
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Botched vaccinations kill 15 children in South Sudan
Same syringe used throughout 4-day campaign, measles vaccine not refrigerated
June 2, 2017
https://www.infowars.com/botched-vaccinations-kill-15-children-in-south-sudan/
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The True Causes of Sudden Infant Death Syndrome
Tremendous Evidence Proves Vaccines Can Cause Sudden Infant Death Syndrome
While medical groups maintain that vaccines have nothing to do with SIDS, independent research and statistical data supports the opposite.
In a detailed report by Miller and Goldman in Human and Experimental Toxicology, the researchers found significantly that sudden infant death syndrome was nonexistent prior to the 1960s and the creation of new multiple infant vaccinations. The United States now has the highest infant vaccination rate of 26 vaccines prior to age one, when compared to 33 other industrialized countries.
The US also has the highest infant mortality rates. Miller and Goodman also found that two thirds of infants who died from SIDS had been vaccinated with the DPT vaccine prior to their deaths.
Their research paper also explains the reported decline in SIDS as being caused by diagnostic changes for SIDS cases, not by an actual decline.
Other Possible Causes of SIDS
An accumulation of other toxic exposures are suspected as contributing to SIDS, with vaccinations being one of the most prominent toxins. Other toxic exposures which overload an infant’s immune system can include pain medication and induction drugs given to the mother at birth. It makes sense that medications, toxic foods, fluoridated water and other toxins in our environment could all work to overload an infant’s immune system.Another theory is that the fungi which grows in PVC, a soft plastic commonly used as a mattress covering, can cause SIDS. Another suspect is the use of flame retardant chemicals for mattresses, required after 1950, about the same time that infant deaths skyrocketed.
https://vactruth.com/2017/01/22/the-true-causes-of-sids/
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California Infant Dies after 8 Vaccines, Family Gets Him Back from Hospital Cremated
4-26-16
https://vactruth.com/2015/02/26/infant-dies-after-8-vaccines/
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13 Year-Old Boy Permanently Disabled from Chicken Pox Vaccine Wins His Case in Vaccine Court
https://vactruth.com/2016/12/30/boy-permanently-disabled-wins-court-case/
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The Most Dangerous Vaccine
Dec 11, 2002
Dan Rather Reports On The Debate Over Safety Of Smallpox Vaccine
The smallpox vaccine is made from a weak biological cousin of the smallpox virus. When you get vaccinated with the weaker virus, you become immune to the smallpox virus. But once in a while, the vaccine does more harm than good. If you scratch where the smallpox is at the surface, and you put it to the eye, you can transfer the smallpox to your eye. That occurs in about 500 people for every million that get the vaccine. If you get "progressive vaccinia," your immune system is compromised. The virus just continues to grow and grow, and is often the cause of death.
No one is certain how many people will be hurt by the vaccine. A 1969 study found that, out of every one million people vaccinated, 74 will suffer serious complications, and at least one will die.
http://www.cbsnews.com/news/the-most-dangerous-vaccine/
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Video Proof - Dangerous Vaccine Paralyzes 40 Children, Prime Minister Visits
Jan 13, 2013
https://www.youtube.com/watch?v=ZEBGG7KFQpU
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Vaccine Dangers
http://educate-yourself.org/vcd/
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Vaccinations: Some Hidden Dangers For Your Child
Few people realize that vaccines contain many chemical additives in addition to the infectious organism being targeted. A typical vaccine could include aluminum, mercury, hydrolyzed proteins, monosodium glutamate, oils, and many complex molecules known as immune adjuvants. Several of these are known to be directly toxic to the brain.
Raymond Francis, M.Sc., from M.I.T., in his book Never Be Sick Again, states: “Vaccines are toxic mixtures loaded with various substances that never should be injected into a human body – including foreign proteins and dangerous viruses from chickens, guinea pigs, calves and monkeys. Some researchers believe these viruses put a permanent burden on our immune systems and do continuous damage both to the immune and nervous systems.”
Today, children get 12 such injections before their first birthday! One doesn’t have to be a brain surgeon to realize the possible damage to a young developing brain and immune system.
One note of vital importance: We talk to many parents of autistic children who state, in no uncertain terms, that their child developed symptoms along the autistic spectrum after the MMR (measles, mumps, rubella) injection. Dr. Bernard Rimland and others noticed that the explosion of autism cases in the U.S. started after the introduction of the MMR to an already overcrowded vaccination schedule.
In 2004, Drs. G.S. Goldman and F.E. Yazbak performed an MMR study in Denmark. Their findings? – A 470% increased incidence of autism since the introduction of the MMR vaccine.
We believe, and the research verifies, that all chronic, degenerative disorders – including autism – are triggered, in large part, by the cumulative toxic burden on the body in individuals with certain genetic predispositions. It’s said that “Genetics loads the gun, but environment pulls the trigger.”
This is the main reason why we recommend using bentonite clay baths to remove toxins. They have been shown to be safe and effective for infants, children and adults in decreasing the toxic load of all pollutants in the body, including heavy metals and vaccination toxins.
https://www.evenbetterhealth.com/vaccination-dangers-for-your-child.php
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How Pharmaceutical Companies Hide the Dangers of Vaccines from Parents
Vaccine horror stories are everywhere these days: Stories of young girls fainting in the doctor offices after receiving the HPV vaccines. Stories of mothers taking a healthy child for a round of shots to their pediatrician returning home with a severely sick or dead child. Stories of children receiving the chicken pox vaccine and experiencing severe cases of chicken pox months later. A 1:50 rate of autism in the United States, increasing autoimmune disorders, seizures, allergies and many other illnesses and disorders. Despite all this, your pediatrician, health officials, governments and pharmaceutical companies proclaim that vaccines are very safe. Did you ever wonder how they derive at such faulty conclusions?
https://vactruth.com/2013/09/26/how-pharmaceutical-hide-dangers/
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The Danger of Excessive Vaccination During Brain Development
March 14, 2008
http://articles.mercola.com/sites/articles/archive/2008/03/14/the-danger-of-excessive-vaccination-during-brain-development.aspx
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Why Do Children Receive So Many Vaccines at Once- CDC Exposed! Nov. 29, 2012
Dec 2, 2012
https://www.youtube.com/watch?v=IiQX0dRejz8
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Kids Given Vaccines Have 22 Times the Rate of Ear Infections
November 01, 2011
32
times the rate of sinusitis, 4x allergies, 2x asthma, 4x hayfever, 3x
ADHD and 19 times higher odds of Autism based on this 7,850 person
survey. Yet 98% of parents give it to their children -...
Vaccinated vs. Unvaccinated: Survey Reveals Who's Healthier
However, that doesn't mean there is a total absence of evidence about the health of vaccinated versus unvaccinated children to give us an indication of whether or not the use of many more vaccines by children is contributing to their being chronically ill. In December 2010, a survey was initiated by VaccineInjury.info3 to compare the health of vaccinated children with unvaccinated children. To date, over 7,850 surveys have been submitted, and the study is ongoing, so if you have an unvaccinated child (or are unvaccinated yourself) and would like to submit your child's health data, you can do so here.Though this is obviously not a double-blind controlled study, and depends on the individuals submitting the data to give accurate information, it is still revealing. So far, the results show:
Health Condition Prevalence in Vaccinated Children Prevalence in Unvaccinated Children
Allergies: 40% report at least one allergy | Less than 10%
Asthma: 6% | 2.5%
Hay fever: 10.7% of German children | 2.5%
Neurodermatitis (an autoimmune disorder): 13% of German children | 7%
ADHD: 8% of German children, and another nearly 6% with borderline cases | 1-2%
Middle ear infections: 11% of German children | Less than 0.5%
Sinusitis: Over 32% of German children | Less than 1%
Autism: Approximately 1 in 100 | Only 4 cases out of 7,800+ surveys (one child tested very high for metals, and another's mother tested very high for mercury)
http://articles.mercola.com/sites/articles/archive/2011/11/01/more-parents-waking-up-to-vaccine-dangers.aspx
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Vaccines Killing Children Worldwide
What they don’t want you to know
Rob Dew | Infowars.com - September 11, 2015
https://www.infowars.com/vaccines-killing-children-worldwide/
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Top gov’t scientists say no to vaccines for their kids
Call in the FBI and DHS. Surround Los Alamos Labs with tanks. Lock the place down
March 26, 2015
https://www.infowars.com/top-govt-scientists-say-no-to-vaccines-for-their-kids/
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Only 121 Of 434 Members Of Congress Admit To Vaccinating Their Child
2/07/2016
http://vaxxter.com/only-121-of-434-members-of-congress-admit-to-vaccinating-their-child/
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German Chancellor, Ministers Get Special Vaccine Without Soft Kill Ingredients
October 17, 2009
Spiegel Online is reporting that German Chancellor Angela Merkel and government ministers will receive a special, additive-free H1N1 vaccine. “The Vakzin [vaccine] does not contain disputed additives — contrary to the vaccine for the remainder of the population,” reports the newspaper.
| Angela Merkel, the German Chancellor, is shown here hobnobbing with fellow minions of the elite, Gordon Brown and Nicolas Sarkozy. | |
“Critics argue that Adjuvantien [adjuvants in the vaccine] could lead to increased inoculation reactions such as headache or fever.” The German government elite and the armed forces will receive Celvapan, an adjuvant-free vaccine manufactured by Baxter. The German public will receive a vaccine produced by GlaxoSmithKline with adjuvants.
Employees of the Paul Ehrlich Institute will also get the adjuvant-free vaccine. Johannes Löwer, president of the institute, said in August that the vaccine causes worse side effects than the virus. Löwer’s comment came after German lung specialist Wolfgang Wodarg said the vaccine increases the risk of cancer. The nutrient solution for the vaccine consists of cancerous cells from animals.
https://www.infowars.com/german-chancellor-ministers-get-special-vaccine-without-soft-kill-ingredients/
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Doctors & Scientists Caution Against Dangers of Vaccination (Immunisation)
http://www.medicinekillsmillions.com/articles/doctors-scientists-warn-dangers-of-vaccination-immunisation.html
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Are Vaccines Safe? Exposing the Dangers of Vaccines
http://dangersofvaccines.com/
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Vaccine Injuries and Death Payments
Report from the Department of Justice
March 5, 2015
http://www.angelfire.com/az/sthurston/vaccination_books.html
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Chapter 3: HPV Vaccine (Gardasil)
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Pfizer VP Comes Clean & Tells The Truth About Pharmaceutical Companies (Video)
Below is a clip taken from the One More Girl documentary, a film that examines the Gardasil vaccine, which was designed to prevent Human Papillomavirus. In it, Dr. Peter Rost, MD, a former vice president of one of the largest pharmaceutical companies in the world (Pfizer), shares the truth about the ties between the medical and pharmaceutical industry.
http://www.collective-evolution.com/2016/05/14/pfizer-vp-comes-clean-tells-the-truth-about-pharmaceutical-companies-video/
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Genetically Modified Virus in HPV Vaccines Causes Twice the Adverse Effects
http://www.collective-evolution.com/2015/07/17/genetically-modified-virus-in-hpv-vaccines-causes-twice-the-adverse-effects/
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New Study Links Aluminum Adjuvant Via HPV Vaccine To Neuroinflammation & Autoimmune Reactions
http://www.collective-evolution.com/2017/02/10/new-study-finds-the-hpv-vaccine-can-trigger-neuroin%ef%ac%82ammation-autoimmune-reactions-leading-to-behavioural-changes/
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CDC updates immunization schedule: New Gardasil Doubles Aluminum
A new super version of the HPV vaccine, which is called HPV9, will translate into a more jarring rush of aluminum for those who decide to take it. It will be sold by the name of Gardasil 9.
http://vaxxter.com/cdc-updates-immunization-schedule-new-gardasil-doubles-aluminum/
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Girl’s Ovaries Destroyed By Gardasil: Merck Did Not Research Effects of Vaccine On Female Reproduction
April 5, 2017
“When one looks at the independent literature, so, studies which are not sponsored by the vaccine manufactures . . with relation to Gardasil, there have been several reports documenting multiple sclerosis and encephalitis, which is brain inflammation, in girls who have received their Gardasil vaccine. So, just because a study sponsored by the manufactures does not identify problems with the vaccine, does not necessarily mean that the vaccine is safe. In fact if one looks at the manufacturer studies, they’re often not designed to detect serious adverse events.”
http://www.collective-evolution.com/2017/04/05/girls-ovaries-destroyed-by-gardasil-merck-did-not-research-effects-of-vaccine-on-female-reproduction/
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13 Year Old Boy Becomes Paralyzed From Neck Down After Gardasil HPV Vaccine
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This Mother Wants You To See What An HPV Vaccine Injury Looks Like
http://www.collective-evolution.com/2015/07/20/this-mother-wants-you-to-see-what-an-hpv-vaccine-injury-looks-like/
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The Adverse, Often Horrific Effects of HPV Vaccine EXPOSED in Ireland
Oct 11, 2015
https://www.youtube.com/watch?v=SkKS76A_K-A
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Concordia Professor Criticizes HPV Vaccine After Winning A Federal Grant To Study It
http://www.collective-evolution.com/2015/10/17/concordia-professor-critisizes-hpv-vaccine-after-winning-a-federal-grant-to-study-it/
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Polish Study Confirms Vaccines Can Cause Large Number of Adverse Effects
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HPV Vaccine Exposed As Japan Health Authorities Withdraw Recommendation To Vaccinate
http://www.collective-evolution.com/2013/07/09/ubc-researchers-expose-gardasil-vaccination-japan-health-authorities-withdraw-recommendation-to-vaccinate/
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The Dangers of the HPV vaccines Gardasil & Cervarix (HPV Vaccination Side Effects Cervical Cancer)
Aug 4, 2012
https://www.youtube.com/watch?v=WCA5haGU6sI
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It's officiall: HPV vaccine, the most dangerous vaccine yet
nyone daring to suggest that a vaccine might present a risk, especially to children as the most vulnerable members of our society, are usually shot down in flames. Health authorities have tried their best to continue telling everyone that HPV vaccines (note use of plural as there are three different types available) are safe, despite ongoing research suggesting otherwise.A study just released by a World Health Organization (WHO) monitoring centre in Sweden shows that adverse event reports received from national authorities — and these will represent only a fraction of those actually experienced — show a tendency to produce clusters of serious adverse events that include complex regional pain syndrome (CRPS), postural orthostatic tachycardia syndrome (POTS) and chronic fatigue syndrome (CFS) that exceeds any other vaccine.
GMO vaccine released a decade ago
The genetically engineered vaccine, first introduced for mass vaccination around 10 years ago, has been delivered to around 80 million girls, women and, in some countries, boys.
By August 2014 58 countries had introduced the vaccine into their immunisation schedules. According to the WHO, 80% of all cases of cervical cancer are linked to HPV, the key justification for giving the vaccine to young girls, occur in developing countries and are linked to sexual encounters at very young ages.
Concerns around the safety of the vaccine began to arise in 2013 with reports of CRPS in Japan, POTS in Denmark and CFS in Holland.
This study, which explores global reporting patterns of Adverse Reactions (AEs) for HPV vaccines shows that these different types of effects are often clustered, i.e. they are more likely to occur together, and so are likely related.
The science bit
The study looked at case safety reports for HPV Vaccine in VigiBase, the WHO International database of suspected adverse drug reactions. Reports are received from pharmacovigilance centres in 124 countries who participate in the WHO Programme for International Drug Monitoring up to 1st January 2015. Cases including at least 2 reporting terms were included in the study.http://anhinternational.org/2017/01/18/official-hpv-vaccine-vaccine-dangerous-yet/
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Merck’s Former Doctor Predicts Gardasil To Become The Greatest Medical Scandal Of All Time
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Cannabis Treats HPV Vaccine Side Effects, Study Suggests
http://www.collective-evolution.com/2017/07/11/cannabis-treats-hpv-vaccine-side-effects-study-suggests/
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Gardassil
http://www.collective-evolution.com/page/4/?s=gardasil
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We even have recent articles from the mainstream media, such as the BBC claiming that not giving HPV vaccines to boys is causing a huge concern. This BBC article does not mention any of the problems listed above with the HPV vaccine shots, and why many people are concerned over the safety of these HPV vaccines. We need to take the time as a civilization to come to terms of what ingredients are in these vaccines, including many of the problems and reactions. We think that vaccines can be used for good purposes, and that we need to stop the problems with many of the bad vaccines and bad medicines on the market.
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Plan not to give HPV vaccine to boys causes concern
- 19 July 2017
A decision not to vaccinate boys against a cancer-causing sexually transmitted infection has attracted fierce criticism.
Reported
cases of human papilloma virus (HPV) - thought to cause about 80% of
cervical cancers - have fallen sharply since girls were given the
vaccine.But the Joint Committee on Vaccination and Immunisation (JCVI) found little evidence to justify treating boys too.
Critics said vaccinating boys could help reduce the risk still further.
Doctor's call to 'equally protect' boys with HPV vaccine
Girls' cancer jab could help also boys, say teachers
HPV vaccinations could lead to 90% drop in cervical cancer
http://www.bbc.com/news/health-40658791
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Chapter 4: Pet & Animal Vaccines
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Are dog vaccines making pets sick?
Feb 24, 2015
CHICAGO (WLS)
The ABC7 I-Team
investigated claims of dangerous side effects from dog vaccines.
Vaccines are considered a standard part of owning a puppy, but some pet
owners say these routine shots are harming their animal.The current human measles outbreak has been partly blamed on the rejection of vaccines. Now, dog owners are questioning the shots their animals are getting. The I-Team has learned of growing concerns about the number of routine shots, and whether they're harming pets health instead of protecting it.
"She's not quite the same dog she used to be," said Julie Harding, owner of a 4-year-old Vizsla named Piper.
This past summer, Harding says Piper became violent and vicious, with unexplained seizures.
"She was foaming at the mouth, she was flailing everywhere," Harding said.
Neurologists at a specialized clinic diagnosed Piper with auto-immune meningitis.
"They asked has she had a vaccine recently," Harding said.
Piper was vaccinated against leptospirosis, a potentially deadly disease. This report says the brain swelling could have been triggered by that vaccine and her predisposition to allergic conditions. Veterinary neurologists say it was unusual to receive Piper's case and four others possibly linked to one vaccine within a month-and-a-half. The manufacturer tells the I-Team each suspected adverse reaction was thoroughly reviewed and no causal association between the vaccine and the Chicago cases was found.
"In the last year to year-and-a-half, we have seen more cases in this practice related to dogs who have recently been vaccinated. We are trying to understand what the risks are," said Michael Podell, D.V.M., a veterinarian neurologist and neurosurgeon.
http://abc7chicago.com/pets/are-vaccines-making-dogs-sick/531640/
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Dog owners' concerns over dog deaths
2 July 2016
Thousands of dogs across Britain are dying or suffering severe allergic reactions after being treated with a vaccine meant to protect them against mild bacterial infections, claim their owners.
Fears over the safety of the vaccine against leptospirosis - a bacterial infection spread to dogs through rats and wild animals – have now led veterinary organisations to issue warnings about its side effects.
According to reports made to the Government's Veterinary Medicines Directorate (VMD) by pet owners, more than 120 dogs are feared to have died after receiving a dosage in the three years the product has been on the market.
In the last two years, regulators have received 2,000 reports of dogs having suspected adverse or fatal reactions.
Whilst leptospirosis can cause kidney problems and death in extreme cases, it is not recommended as a “core” vaccine to be given routinely.
Owners that have opted for the L4 vaccine, which is registered for use across Europe, have reported adverse effects including epileptic fits, swollen glands and blindness.
http://www.telegraph.co.uk/news/2016/07/02/dogs-dying-after-having-protective-vaccine-owners-claim/
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Vaccines 'are making our dogs sick as vets cash in'
http://www.dailymail.co.uk/news/article-1255863/Vaccines-making-dogs-sick-vets-cash-in.html
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Adverse Reactions to Vaccination
Veterinary & Aquatic Services Department, Drs. Foster & Smith
Anaphylaxis
Anaphylaxis is a rare, life-threatening, immediate allergic reaction to something ingested or injected. If untreated, it results in shock, respiratory and cardiac failure, and death. An anaphylactic reaction can occur as a result of vaccination. The reaction usually occurs within minutes to hours (less than 24) of the vaccination. Dr. Ronald Schultz of the University of Wisconsin College of Veterinary Medicine estimates that about one case of anaphylaxis occurs for every 15,000 doses of vaccine administered.
The most common symptoms of anaphylaxis are the sudden onset of diarrhea, vomiting, shock, seizures, coma, and death. The animals' gums will be very pale, and the limbs will feel cold. The heart rate is generally very fast, but the pulse is weak. There can be facial swelling.
Anaphylaxis is an extreme emergency. If you think your dog is having an anaphylactic reaction, seek emergency veterinary assistance immediately. Epinephrine should be given as soon as possible - we are talking within a few minutes. IV fluids, oxygen, and other medications are given as needed.
Anaphylactic reactions are more commonly associated with the use of killed vaccines such as rabies, canine coronavirus, and leptospirosis. Killed vaccines have more virus or bacterial particles per dose and have added chemicals (adjuvants) to improve the dog's immune response. These characteristics also increase the risk of an allergic reaction to the vaccine.
Neurologic and eye disease
Neurologic symptoms are the most common vaccine reaction seen in dogs. Canine distemper vaccination is the most common cause of neurologic disease, and can cause an inflammation of the brain. Measles vaccine in puppies has been reported to rarely cause damage to the nervous system. Cerebellar disease has been reported in puppies less than 5 weeks of age who were vaccinated with a modified live vaccine.
Canine adenovirus-1 is known to cause an allergic uveitis (inflammation of the eye), often called 'blue eye.' Most vaccines now contain canine adenovirus-2 instead of adenovirus-1, almost eliminating the chance of blue eye occurring today.
Mild fever, decreased appetite and activity
Mild fever, decreased appetite, and depression may be observed for 1-2 days following vaccination, most commonly when modified live vaccines are used. Generally, no treatment is warranted.
Severe illness can occur if vaccines designed for intranasal use are accidentally injected. Severe reactions can also occur if any of a vaccine made for injection accidentally enters an animal's eyes, nose, or mouth.
Respiratory signs after intranasal vaccines
Dogs vaccinated with the intranasal Bordetella and/or parainfluenza vaccine may develop a mild cough, which generally does not require treatment. They may spread the vaccine-form of the virus to other animals through their coughing.
Lameness
Rarely, lameness can result from several different vaccinations.
Immune-mediated polyarthritis in Akitas: Certain lines of Akitas may have immunodeficiencies which make them prone to adverse reactions following vaccination. They may develop an immune-mediated arthritis in one or more joints, which is often progressive and relapses commonly occur. Dogs with this immune disorder generally have short life spans due to other complications.
Hypertrophic osteodystrophy: Certain lines of Weimaraners, and some other large-breed dogs, may develop hypertrophic osteodystrophy following canine distemper vaccinations given between 2 and 5 months of age. They may also develop respiratory signs, enlarged lymph nodes, and diarrhea. The hypertrophic osteodystrophy is treated with glucocorticoids and the signs of the disease usually resolve.
Shedding of vaccine agent
Vaccine virus may be found in the nasal secretions of dogs vaccinated intranasally. In addition, vaccine parvovirus is shed in the feces of vaccinated dogs, canine adenovirus-1 can be shed in the urine, and canine adenovirus-2 can be found in nasal secretions. These viruses are the vaccine forms of the virus; they do NOT revert back to the disease-causing strains.
Birth defects or infections
The vaccination of pregnant animals with a modified live vaccine can result in birth defects or abortions. It is recommended that modified live vaccines NEVER be given to pregnant animals.
http://www.peteducation.com/article.cfm?c=2+2115&aid=962
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The Leptospirosis Vaccine: Why It Doesn’t Work
What Is Leptospirosis?
Leptospirosis is an infection caused by Leptospira bacteria.Within the genus Leptospira there are 20 different species and more than 200 different serovars (a group of closely related microorganisms with a common set of antigens).
Dogs can catch Leptospira bacteria from water or soil that is contaminated with infected urine from rodents and other wild animals.
If your dog spends a lot of time playing in ponds or lakes or drinking out of puddles or standing water, he may be at risk, depending on the incidence of Leptospirosis in your area.
The Leptospirosis Vaccine
There are many problems with the Leptospirosis vaccine, which is why many vets stay away from it. The two most important strikes against it are …- It does not provide effective immunization
- It has an extremely high rate of adverse reactions
http://www.dogsnaturallymagazine.com/leptospirosis-vaccine-side-effects/
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NEWS: Reports Show UK Dogs Are Dying From Lepto Vaccine
UK dog owners are learning what US dog owners have suspected all along …
…the lepto vaccine is much more dangerous than we’re led to believe.
The Nobivac L4 lepto vaccine, which was rolled out in the UK by Merck’s UK subsidiary, MSD Animal Health, is reportedly causing adverse effects in the dogs receiving it, including epilepsy, swollen glands, blindness and death.
http://www.dogsnaturallymagazine.com/report-lepto-vaccine-uk-dogs-dying/
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What is Canine Leptospirosis and How Dangerous is the Vaccine for It?
http://barkandswagger.com/what-is-canine-leptospirosis-and-how-dangerous-is-the-vaccine-for-it
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How Mystery Ingredients In Vaccines Can Harm Your Dog
Dog Vaccinations
If you’ve ever asked your conventional vet about the ingredients in dog vaccines, he probably told you they’re generally safe for your dog and that vaccine reactions are rare.
The fact is, your vet can’t say for sure what’s in your dog’s vaccines … because he doesn’t know.
Nobody does. Not even the US Food and Drug Administration (FDA), who’s responsible for allowing these drugs on the market.
That’s because the manufacturers hide behind descriptions like “trade secret” or “proprietary” when they report what’s in the vaccines. It’s shocking that the government agency responsible for drug safety lets companies get away with hiding their ingredients. But they do, as you can see from this extract from the Material Safety Data Sheet for the RabVac® 3 vaccine:
http://www.dogsnaturallymagazine.com/mystery-ingredients-in-dog-vaccines/
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Side Effects of the Canine DA2PP Vaccine
Vaccinations offer dogs great protection against many serious diseases, however, at times they may also cause unpleasant side effects. The DA2PP vaccine is a combination shot protecting against four diseases: distemper, adenovirus 2, parainfluenza and parvovirus. Should your dog develop any side effects following vaccination, report them to your veterinarian immediately.
Anaphylactic Shock
Anaphylactic
shock is a sudden, life-threatening allergic reaction triggered by the
dog's immune system as a response to some portion of the vaccine.
Symptoms of anaphlylactic shock are vomiting, diarrhea, facial swelling,
itching, trouble breathing, weakness, seizures, and even death,
explains Dr. Lila Miller, a veterinarian for the ASPCA, in an article
for Pet Finder. Should your dog develop any of these symptoms following
the DA2PP vaccine, seek emergency veterinarian care immediately.
Systemic Reactions
Some
dogs may appear to be lethargic, less playful and may sleep more
following the DA2PP shot . Loss of appetite may accompany these
symptoms. Some dogs may also react with a slightly elevated temperature.
Generally, these mild reactions tend to develop one to two days
following vaccination, are short-lived and usually resolve without
treatment, according to Pet Place veterinarians.
Localized Reactions
Other Side Effects
Some dogs have been known to develop nervous system problems after the administration of a modified live distemper vaccine. This condition is known as "vaccinial distemper" and takes place 10 to 21 days following the modified live vaccine, according to Mar Vista Animal Medical Center. In some cases, dogs have also developed autoimmune hemolytic anemia, a condition where antibodies attack the body's own red blood cells, leaving the dog anemic. This condition has been seen particularly following vaccination against parvo.
https://www.cuteness.com/article/side-effects-canine-da2pp-vaccine
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Is the Canine Bordetella Vaccine Safe for Dogs?
Jan 7th 2014
There are two additional controversies surrounding the Bordetella vaccine. The first one relates to how frequently it should be administered. The most common current recommendation is that dogs receive Bordetella boosters every six months. However, Dr. Ford points out that “there are no studies demonstrating the value” of doing this. He suspects that annual vaccination may be more appropriate.
The second controversy is whether dogs should receive Bordetella vaccines at all. The vaccines are designed to reduce the risk of an infection that is generally mild and self-limiting. And vaccination certainly doesn’t guarantee that a dog won’t contract kennel cough — as I mentioned, there are dozens of bugs other than Bordetella that can cause the syndrome.
Many boarding and grooming facilities require that dogs be vaccinated against Bordetella prior to entry. If your dog will frequent such a facility, then he or she will probably be getting the vaccine. But I do not generally recommend Bordetella vaccines as part of routine vaccination protocols for dogs that don’t frequent such facilities.
http://www.dogster.com/lifestyle/dog-health-canine-bordetella-vaccine-vaccinations-ask-a-vet
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Human Illness Associated with Use of Veterinary Vaccines
Veterinary vaccines are being used with increasing frequency in the United States to protect the health of animals. In addition to their direct benefit to animals, these vaccines have also markedly decreased the risk of transmission of many zoonotic infections (e.g., rabies and brucellosis) to humans. The US Department of Agriculture currently licenses >2000 vaccines for use in animals. Most of these vaccines are inactivated formulations, but >500 live vaccine formulations for animals are also licensed. Veterinary vaccines are intended only for use in animals and are not tested for safety in humans. However, humans may inadvertently be exposed to these products by means of unintentional inoculation or other routes of exposure.
Vaccinia Infection Associated With Use of Oral Rabies Wildlife Vaccine
To control the spread of rabies in wildlife populations, live-virus vaccines containing either modified live rabies virus or recombinant vaccinia-rabies glycoprotein virus are placed in oral baits that are then widely distributed in parts of North America and Europe; the recombinant vaccinia-rabies glycoprotein virus is used in baits in the United States. The use of such baits continues to increase, and, until 2000, no adverse events in humans were reported as having been due to human exposure to the vaccine-containing baits. More than 3 million baits had been distributed in Ohio since the beginning of 1997, with >22 million baits distributed nationwide from 1990 through 2000. In Ohio, toll-free numbers are printed on baits, and callers can reports baits if they are found. In 20 of 160 reports of contact with a bait, persons reported exposure to the vaccine, with evidence that the inner sachet containing the vaccine had ruptured. Two of the 20 instances of exposure likely involved persons with a contraindication to vaccination with the vaccinia virus. In 1 of these 2 instances, a 26-year-old woman was exposed to the recombinant vaccinia-rabies glycoprotein virus vaccine, which led to severe illness and resulted in hospitalization. The woman was 15 weeks pregnant and had epidermolytic hyperkeratosis. She recovered and later delivered a healthy infant who had no evidence of infection.The case of vaccinia virus infection in this woman highlights the risk associated with vaccines that contain vaccinia virus, including animal vaccines.
A tremendous amount of attention has recently been given to potential adverse events associated with vaccinia vaccine for the protection of individuals against smallpox. The risk of contact transmission of vaccinia virus via smallpox vaccination has been estimated to be 2–6 cases per 100,000 primary vaccinations, with 1 to 2 cases of eczema vaccinatum resulting from such transmission per 100,000 primary vaccinations. Relatively little attention, however, has been paid to the increasing rate of exposure of humans to vaccinia virus due to contact with wildlife baits. The vaccinia virus strain used in wildlife vaccine may be less virulent than the strain used in the smallpox vaccine currently licensed for human use in the United States. There was only 1 reported case of vaccinia virus infection among 160 reports of human contact with ⩾1 of the 3.8 million baits dropped in Ohio; however, a marked increase in the use of baits may result in more cases of human vaccinia virus infection, including eczema vaccinatum.
Occupational Exposure to Vaccines
Unintentional, Nonoccupational Exposure of Humans to Animal Vaccine
Intentional Administration of Animal Vaccines to Humans
https://academic.oup.com/cid/article/37/3/407/437242/Human-Illness-Associated-with-Use-of-Veterinary
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Caution Is Urged on Lyme Disease Vaccinations for Dogs
June 22, 1991
Scientists have also raised concerns about possible
longer-term dangers. Evidence is growing that some ill effects of Lyme
disease in humans are not caused by the bacterium directly, but by the
responses to it of the body's immune system -- autoimmune effects --
said Dr. Richard H. Jacobson, a veterinary scientist at Cornell
University. In theory, he vaccine might promote similar effects over
time, he said. Fort Dodge scientists respond that laboratory studies and
monitoring of the thousands of dogs receiving the vaccine have shown no
evidence of such an effect.
The choice, as Dr. Jacobson sees it, is between
administering the vaccine despite possible risks, or taking the chance
that an unvaccinated dog will get Lyme disease and then treating it. "My
point is, since antibiotic therapy is so effective, why not go that
route?" Dr. Jacobson said.
Many scientists are wondering about the vaccine's effectiveness under field conditions, where dogs are often repeatedly infected by ticks in contrast to the injections of live bacteria in the Fort Dodge studies. It is also unclear how much protection is offered to dogs that were previously infected with Lyme disease.
http://www.nytimes.com/1991/06/22/news/caution-is-urged-on-lyme-disease-vaccinations-for-dogs.html--------------------------------------------
Lyme Vaccines Show New Promise, and Face Old Challenges
10.02.2019
The first human Lyme vaccine was pulled off the market nearly twenty years ago. A new effort faces lingering suspicions.
https://undark.org/article/new-landscape-lyme-vaccines/?utm_source=pocket-newtab
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Vaccinosis: Health Hazards of Scheduled Animal and Pet Vaccines
Most pet owners aren't aware of the dangers in animal vaccinations that have been discovered in recent years. Major veterinary associations now agree that immunizations can trigger all sorts of maladies, from allergies to cancer - but most pet caretakers (and many veterinarians, it seems) haven't gotten the word. "With vaccines that are repeated year after year, the frequency and severity of these side-effects in our pets has increased dramatically." Dr. Donna Starita Mehan, DVM.
1 in 10 Risk of Adverse Reactions from Vaccines
Journal of the American Veterinary Medical Association - Adverse events diagnosed within three days of vaccine administration in dogs study of more than 2,000 cats and dogs in the United Kingdom by Canine Health Concern showed a 1 in 10 risk of adverse reactions from vaccines. This contradicts what the vaccine manufacturers report for rates of adverse reactions, which is “less than 15 adverse reactions in 100,000 animals vaccinated” (0.015 percent). Additionally, adverse reactions of small breeds are 10 times higher than large breeds, suggesting standard vaccine doses are too high for smaller animals.
http://www.shirleys-wellness-cafe.com/AnimalWellness/Petvacc
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Five Dangerous Dog Vaccine Ingredients
1. Aluminium
2. Thimerosal
3. Contaminants
Contaminants found in vaccines are also behind many of the adverse reactions we see in dogs. “Contaminant” means anything that shouldn’t be there. That’s anything impure or unclean, is toxic or poisonous, or has the ability to create disease. Vaccines contain contaminants that can cause cancer, leukemia, autoimmune diseases and a myriad of other unwanted conditions.
An important scientific paper was published in April 2010 in the Journal of Virology (Isolation of an Infectious Endogenous Retrovirus [RD-114] in a Proportion of Live Attenuated Vaccines for Pets, Journal of Virology, April 2010, p 3690-3694, Vol 84, No 7). It showed how two teams of scientists, in Japan and the UK, isolated a feline retrovirus (called RD-114) in both feline and canine vaccines in the UK and Japan. Had teams from America, or Germany, or Kazakhstan also been looking, they would probably have found the retrovirus, too. The contamination involved seed stock – the witches’ brew of disease shared amongst vaccine manufacturers internationally, from which they make their vaccines.
The following are extracts from a related paper appearing in Biologicals in 2010. “RD-114 was first isolated from a human tumor cell line (RD cells) derived from a human rhabdomyosarcoma after passage through fetal cats, and is thought to be xenotropic.”
Translation: they found this cat retrovirus in a highly malignant human tumor. “Xenotropic” means that it will be harmless in the original host species, but will cause problems (like tumors) in a different species.
In her article on Vaccine Contaminants in the January 2013 issue of Dogs Naturally Magazine, author Catherine O’Driscoll continues, “One of the authors of this paper wrote to me privately: “If the ERV induces diseases in vaccinated animals and humans, it will take more than five years (in animals) to ten years (in humans) when the first patient appears. But it will take additional time to relate some diseases with specific vaccines because expected diseases are very common (such as cancers, lymphoma and autoimmune diseases). If so, when we are aware of the real risk of ERVs, it is too late because millions are infected with the viruses by the contaminated vaccines.””
The only official checks made for contaminants in vaccines are for a few known pathogens, potentially missing a vast host of unknown, unstudied, small particles and chemicals. It’s simply impossible to remove contaminants from vaccines.
4. Animal Protein
Disease micro-organisms are often cultured on animal tissue including embryonic chickens or cow fetuses. When a vaccine is manufactured, it is impossible to divide the wanted virus from the unwanted animal tissue. It all gets ground up together and injected into your dog’s body.
If a dog eats animal flesh or an egg, it is digested into simpler amino acids before entering the bloodstream. The digestive process in most cases changes protein molecules so they don’t trigger an immune reaction. This is not the case for vaccines. They are injected undigested, directly into the bloodstream, where the foreign protein matter circulates throughout the body.
An immune response is triggered when the body detects foreign proteins. Killer cells (white blood cells) are sent out to consume the cells containing the foreign proteins and protein fragments. This process is nature’s way of protecting the body from being overwhelmed by invading organisms and eventually succumbing to them. The foreign protein fragments are not always destroyed by the body as it is busy cleaning up the multiple viruses that have just been injected, along with the serious chemicals aluminum, Thimerosal, formaldehyde and more. So the foreign protein matter gets absorbed into body cells. T-Cells, sensing they are there, but unable to reach them directly, attack the body cells that harbor them. This can lead to autoimmune disorders including cancer, allergies, arthritis and more.
“Our ongoing studies of dogs show that following routine vaccination, there is a significant level of antibodies dogs produce against their own tissues…Some of these antibodies have been shown to target the thyroid gland, the connective tissue such as that found in the valves of the heart, red blood cells, DNA etc.” Larry Glickman DVM, referring to the results of the
5. Money
The final vaccine ingredient to be discussed isn’t injected into dogs, but the concept of vaccination itself. In 2005, the global vaccine market was $6 billion. In 2012, it is $34 billion. It’s not surprising that more vaccines are manufactured for dogs and media hype frightens pet owners into using them. The canine influenza vaccine is an example.
In 2011, the media heavily covered canine influenza and the need for vaccination. At the center of most of the media articles reporting the need to vaccinate for canine influenza was Dr Cynda Crawford. Dr Crawford is a veterinarian at the University of Florida (UF) who led the research team that first identified the canine influenza virus in 2004.
Interestingly, Crawford, along with colleagues at UF, Cornell University and the U.S. Centers for Disease Control and Prevention (CDC), share intellectual rights to the canine influenza virus; Merck has licensed the right to use the virus to make a vaccine. However, Crawford maintains that she and the others do not receive compensation from vaccine sales.
http://www.dogsnaturallymagazine.com/five-vaccine-ingredients-that-can-harm-your-dog/
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Pet Vaccination: Risks and Benefits
Parvovirus
The Disease
Parvovirus is a common disease that appeared throughout the developed world in the 1970’s as a direct result of vaccination. Being a canine form of Feline Viral Enteritis, it is thought that the virus ‘jumped’ through dogs coming in contact with vaccinated cat’s infectious stool, or mutated during the production of the distemper vaccine which was cultivated on infected cats kidneys, (very similar reports have been made regarding the polio vaccine in humans). The disease is only of real concern in puppies, as 90% of dogs over the age of 8 weeks will survive infection without complication, with death in mature healthy dogs being almost unheard of. While mature dogs usually only experience a type of diarrhea and enteritis, young puppies are at serious risk of heart failure and chronic cardiac problems.
Treatment Options
Vaccinated puppies don’t respond as well to treatment as unvaccinated puppies. Due to the seriousness of symptoms produced by this disease in young pups, an experienced veterinary homeopath should be consulted quickly to determine the most appropriate remedy. While awaiting a consultation, Aconite 30C can be given orally every two hours. It is critical to avoid dehydration, and if this is feared, China 6C or 30C may be given every hour in a little filtered water.
Reasons to Vaccinate
- The parvo vaccine is effective if given after 12 to 16 weeks. If given before this age, the maternal antibodies are likely to block the vaccine.
Reasons not to Vaccinate
- Vaccinating for parvo keep the disease in the environment. There is no vaccine for the original strain of parvovirus, CAV-1 yet dogs no longer get sick from it. The newer strains, which do cause illness in dogs, are the result of mutation due to vaccination. The same issue is happening worldwide with the polio vaccine.
- Like other modified live vaccines, the parvo vaccine has been known to create the disease it was intended to prevent.
- Puppies are likely to be exposed to parvo when brought to the vet’s office for their parvo vaccination. It takes two weeks for the vaccine to protect the puppy, so not only can the vaccine cause parvo in puppies, the trip to the vet’s office can.
- Parvo is more treatable in unvaccinated puppies, especially over the age of 8 weeks. Vaccinating before that age is just as likely to not protect the puppy as it is to protect him.
- The risk of Vaccine Induced Autoimmune Disease is greater than the risk of parvo.
- The parvo vaccine has been linked to heart disease.
- Vaccination suppresses the immune system for several days, increasing the puppy’s risk of developing disease.
- Parvovirus vaccination can create a chronic form of the disease, the symptoms of which include chronic gastritis, hepatitis and pancreatitis, chronic diarrhea and food sensitivities.
Distemper
The Disease
Distemper is a rare but serious viral disease that dogs are still considered at risk from in many developed countries. It affects all aspects of a dog’s health eventually attacking the central nervous system causing spasm, seizures and paralysis. The wide variety of symptoms found under this disease classification is said to be due to the distemper virus’ lowering of the overall immune system which in turn allows for secondary opportunistic infections that produce the diverse clinical symptoms. The virus is thought to be transmitted through the air via infected animal’s breath, although E. H Ruddock DVM states that “all dogs appear to carry the seeds of distemper in their system”.Treatment Options
Canine distemper is a serious disease and, when treated conventionally, 50% of dogs with distemper will die. Homeopathic vets see much better results however, thanks to Distemperinum. Due to the vast array of clinical symptoms produced by this disease, an experienced veterinary homeopath should be consulted immediately to determine the most appropriate remedy. “If the disease is noticed in the early stages, use of the potentised virus by itself may achieve spectacular results” (Macleod). Treatment of dogs who have survived distemper but exhibit ongoing symptoms of paralysis and seizure has been found affective and may include the use of such common remedies as Belladonna, Gelsemium, Conium and Causticum.
Reasons to Vaccinate
- Distemper can have a high mortality rate, without access to a homeopathic vet.
- The distemper vaccine is relatively effective. One dose given to a puppy over 12 weeks of age will protect him within hours and last a lifetime.
- Although no vaccine is safe, distemper is one of the less controversial vaccines.
Reasons not to Vaccinate
- Distemper is a relatively rare disease.
- Like many modified live vaccines, the distemper vaccine has been known to create the disease it was intended to prevent.
- The distemper vaccine has been strongly linked to joint disease and arthritis – two increasingly common chronic diseases in dogs.
- The risk of Vaccine Induced Autoimmune Disease is greater than the risk of distemper.
- The distemper vaccine likely caused the parvovirus outbreaks in the 1970s.
- The distemper vaccine may cause parvo in young puppies.
- Maternal antibodies are likely to block the vaccine until 12 weeks of age.
- Post Vaccinal Encephalitis is a recognized complication of the vaccine.
- Vaccination suppresses the immune system for several days, increasing the puppy’s risk of developing disease.
- The vaccine can cause persistent skin problems and allergies.
- Distemper vaccination can create a chronic form of the disease, the symptoms of which include watery eyes and nose, chronic gastritis, hepatitis and pancreatitis, chronic diarrhea, food sensitivities, epilepsy and rear leg paralysis, spondylitis, lip fold dermatitis, allergic eruptions on the face, eruptions between the toes and a habit of licking the feet, interdigital dermatitis, kennel cough and bronchitis, lack of appetite and failure to thrive.
http://www.dogsnaturallymagazine.com/pet-vaccination-risks-and-benefits/
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The Lepto Vaccine: Why Vets Give It Yearly
Dog Vaccinations
Leptospirosis is feared by many veterinarians because it can cause severe kidney or liver disease in its pet victims. It’s double feared because it’s a zoonotic disease; in other words, you can get it too.
Many vets use this fact to justify the administration of this supposedly protective vaccine. But a good vaccine should be both safe and effective. Let’s examine whether or not the yearly lepto vaccination is either.
Enter Lepto
Because annual vaccination has been pushed for many years, pet guardians have come to believe the only reason to take their pets to the vet annually is for vaccination.Veterinary visits are in decline and when a pet guardian learns they don’t need to vaccinate as frequently, vet visits drop. So, what is a vet to do when their income from annual visits goes down?
Many began promoting a separate leptospirosis component to the core vaccines as the new annual vaccine. But according to the AVMA, leptospirosis isn’t a core vaccine.
This means it’s not recommended for all dogs in all communities. Whether or not this vaccine is important for your dog is left completely up to the discretion of your vet.
In my prior, non-holistic practice, I saw that the lepto component cause the most serious reactions in my patients.
The typical reactions not only included vomiting or diarrhea, but anaphylaxis with shock or death and serious immune-mediated diseases, which may manifest as bleeding disorders.
http://www.dogsnaturallymagazine.com/the-lepto-vaccine-why-vets-give-it-yearly/
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Dangerous Over-Vaccination Is On The Rise
Dog Vaccinations
http://www.dogsnaturallymagazine.com/dangerous-over-vaccination-on-the-rise/
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In Depth Annual Dog Vaccination Dangers
How Often Do You Have Your Dog Vaccinated?
This is an in-depth analysis of the very real dangers of dog and cat vaccinations.If you want a general overview click here. Overview Annual Dog Vaccination Dangers.
What Are Vaccines(3) A vaccine is one or more disease antigens that, when injected into a dog's body, causes his immune system to produce specialised proteins known as immunoglobulins or antibodies.
Antibodies fight infection and disease and neutralise the antigens by binding to them.
The cells that created the antibodies (a form of white blood cell) have a memory of the antigen so that when the antigen is encountered again, the cells' "memory" enables them to rapidly produce more antibodies i.e. to mount immunity against that pathogen.
The most common dog vaccination is a combination cocktail called DHLPPC which includes pathogens for:
• Distemper
• Adenovirus-2
• Leptospirosis
• Parainfluenza
• Parvo
• Coronavirus
all in a single injection. Other vaccinations are often given at the same time are:
• Rabies
• Bordatella (Kennel Cough)
• Lyme Disease (Leptospirosis)
• Giardia
Vaccinations are thought to sometimes overstimulate the immune system, causing it to malfunction. They are contraindicated for dogs with already suppressed immune systems. modified live Vaccine (MLVs) have the potential to revert to the virulent form of the disease.
(3) Studies have shown that most canine vaccinations provide immunity from seven years to life if given when a dog's immune system is mature. However, it appears that vaccinations also have considerable potential for harm.
(3) In 2002, a landmark report published by the American Veterinary Medical Association (AVMA) Council on Biologic and Therapeutic Agents (COBTA) stated in part, "...the practice of re-vaccinating animals annually is largely based on historic precedent supported by minimal scientific data; unnecessary stimulation of the immune system does not result in enhanced disease resistance and may expose animals to unnecessary risks..."
https://www.doglistener.co.uk/depth-annual-dog-vaccination-dangers
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How Much Money are You Wasting on Pet Vaccines?
The ‘Vaccination Business’ is a Major Profit Center for Many Veterinarians
Vet practices which depend on vaccination schedules to keep the doors open are typically small operations of one to three doctors that do not specialize or provide emergency services. The markup on rabies vaccines is astronomical – 2400 to 6200 percent in many cases. Estimates are that removing the one-year rabies vaccination/office visit for dogs alone could reduce a veterinarian’s income from $87,000 to $25,000. And this example involves just one variety of one vaccine, and only dogs.
How Re-Vaccinating Your Pet Can Harm His Health
Once your puppy or kitten is fully immunized against viruses, he is immune for years, and often for a lifetime. Vaccination against bacterial pathogens creates a memory in your pet’s immune system that helps protect him if and when he’s exposed to dangerous organisms. After your pet has received all his puppy shots, the antibodies he develops to the viruses he’s been immunized against will actually protect him from the same viruses introduced in future vaccinations.In other words, if his puppy vaccinations are successful, his immune system response to subsequent vaccinations will fight off their effect, rendering them useless. But vaccines, like any pharmaceutical drug, are not without side effects. So re-vaccinating for the same pathogens year after year is more than just a waste of your money – it also poses ever increasing risks to your pet’s health.
http://healthypets.mercola.com/sites/healthypets/archive/2010/03/31/high-cost-of-pet-vaccinations.aspx
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Vaccination of dogs
https://en.wikipedia.org/wiki/Vaccination_of_dogs
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Vegan Mother Forced To Vaccinate Sons
United Kingdom – A vegan mother who had hoped to keep her son’s vegan, as well, has lost a battle with her former husband to the High Court, which will now force the children to receive a handful of vaccinations.
http://vaxxter.com/vegan-mother-forced-to-vaccinate-sons/
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Pharma’s Brutal Vaccine Testing On Monkeys
7/06/2017
http://vaxxter.com/pharma-has-heroin-vaccine-ready-for-clinical-phase/
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Pharma Found To Have Horse Blood Draining Farms
Sometimes, the Internet can be so
completely outrageous that we almost have to take a few minutes to
confirm that something we read or see is true. And often times, we hope
that the news isn’t true.
So when I heard that pharmaceutical
companies have horse blood farms, my first reaction was anger. My second
reaction was to do some research and find out if this was just some
sort of fake news that would fit the narrative of “evil big pharma.”
And then, my third reaction was back to
anger. That’s because you guessed it, big pharma has horse blood
draining farms. Often they use horseshoe crabs for their blood draining needs. Sometimes they just use us and figure it out as they go along. Now apparently, they have driven up horse blood stock.
So you might be asking, why would
pharmaceutical companies drain horses of their blood? Well,
specifically, pharma extract’s pregnant mares’ blood. They are receiving
in return, a specific hormone they use in their drugs. According to a petition on SumOfUs.com, Merck, Sharpe and Dohme (MSD) all caved under pressure and claim they’ve stopped this activity.
But blood farms will still operate as long as there are pharma companies willing to buy their products. Can you help push the next big player, Germany’s IDT Biologika, to cut ties with blood farms? We’ll be delivering your voices straight to IDT Biologika’s headquarters in less than two weeks — so please add your name now.Blood farm conditions are worse than you’d ever imagine.On farms in Uruguay and Argentina, our partners revealed that workers routinely take 10 litres of blood from the horse in a single extraction — almost a fourth of its total blood. That much blood loss can lead to hypovolemic shock, anaemia or even death.It’s a hormone called PMSG that the pharma industry is after. Because the valuable hormone can only be found in the blood of mares in their early pregnancy, they are forced into repeated pregnancies and abortions. Finally, they are shipped off to be slaughtered once they’re too weak or old to become pregnant.
http://vaxxter.com/pharma-found-to-have-horse-blood-draining-farms/
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Bovine somatotropin
Bovine somatotropin or bovine somatotrophin (abbreviated bST and BST), or bovine growth hormone (BGH), is a peptide hormone produced by cows' pituitary glands.[1] Like other hormones, it is produced in small quantities and is used in regulating metabolic processes.[1] After the biotech company Genentech discovered and patented the gene for BST in the 1970s,[2] it became possible to synthesize the hormone using recombinant DNA technology to create recombinant bovine somatotropin (rBST), recombinant bovine growth hormone (rBGH), or artificial growth hormone. Four large pharmaceutical companies, Monsanto, American Cyanamid, Eli Lilly, and Upjohn, developed commercial rBST products and submitted them to the US Food and Drug Administration (FDA) for approval.[3][4] Monsanto was the first firm to receive approval. Other countries (Mexico, Brazil, India, Russia, and at least ten others) also approved rBST for commercial use.[5] Monsanto licensed Genentech's patent,[2] and marketed their product as "Posilac".[6][7] In October 2008, Monsanto sold this business, in full, to Eli Lilly and Company for $300 million plus additional consideration.
Contents
https://en.wikipedia.org/wiki/Bovine_somatotropin#Controversy
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How drugs pumped into supermarket chickens pose a terrifying threat to our health
In the past, the blame for the growth of drug-resistant superbugs was pinned on doctors who over- prescribed antibiotics to patients.
But there is a growing body of opinion that believes excessive use of the drugs within the agricultural world — especially cheap chicken — is equally to blame.
Scientists are particularly concerned about the over-use of a group of antibiotics called cephalosporins, which they believe are linked to the emergence of drug-resistant strains of E.Coli.
Cephalosporins are a class of antibiotics that the World Health Organisation has rated as ‘critically important to human medicine’.
‘For me the evidence is overwhelming,’ says Professor Collignon. ‘With certain bacteria, what we do with animals is making them resistant.’
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Food, Inc.
Content
The film's first segment examines the industrial production of meat (chicken, beef, and pork), calling it inhumane and economically and environmentally unsustainable. The second segment looks at the industrial production of grains and vegetables (primarily corn and soy beans), again labeling this economically and environmentally unsustainable. The film's third and final segment is about the economic and legal power, such as food labelling regulations, of the major food companies, the profits of which are based on supplying cheap but contaminated food, the heavy use of petroleum-based chemicals (largely pesticides and fertilizers), and the promotion of unhealthy food consumption habits by the American public.[4][7] It shows companies like Wal-Mart transitioning towards organic foods as that industry is booming in the recent health movement.
https://en.wikipedia.org/wiki/Food,_Inc.
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Chapter 5: Genetically modified Vaccines & Viruses
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Genetically engineered vaccines: an overview.
1998
Despite the early success demonstrated with the hepatitis B vaccine, no other recombinant engineered vaccine has been approved for use in humans. It is unlikely that a recombinant vaccine will be developed to replace an existing licensed human vaccine with a proven record of safety and efficacy. This is due to the economic reality of making vaccines for human use. Genetically engineered subunit vaccines are more costly to manufacture than conventional vaccines, since the antigen must be purified to a higher standard than was demanded of older, conventional vaccines. Each vaccine must also be subjected to extensive testing and review by the FDA, as it would be considered a new product. This is costly to a company in terms of both time and money and is unnecessary if a licensed product is already on the market. Although recombinant subunit vaccines hold great promise, they do present some potential limitations. In addition to being less reactogenic, recombinant subunit vaccines have a tendency to be less immunogenic than their conventional counterparts. This can be attributed to these vaccines being held to a higher degree of purity than was traditionally done for an earlier generation of licensed subunit vaccines. Ironically, the contaminants often found in conventional subunit vaccines may have aided in the inflammatory process, which is essential for initiating a vigorous immune response. This potential problem may be overcome by employing one of the many new types of adjuvants that are becoming available for use in humans. Recombinant subunit vaccines may also suffer from being too well-defined, because they are composed of a single antigen. In contrast, conventional vaccines contain trace amounts of other antigens that may aid in conferring an immunity to infectious agents that is more solid than could be provided by a monovalent vaccine. This problem can be minimized, where necessary, by creating recombinant vaccines that are composed of multiple antigens from the same pathogen. These issues are less of a concern with a live attenuated vaccine, since these vaccines are less costly, require fewer steps to manufacture, and elicit long-lived immunity after only a single dose. Unfortunately, live vaccines carry a higher risk of vaccine-induced complications in recipients that make their use in highly developed, litiginous countries unlikely. In lesser developed countries, where the prevalence of disease and the need for effective vaccines outweighs the risk associated with their administration, live vaccines may play an important role in human health. This review has attempted to make the reader aware of some of the current approaches and issues that are associated with the development of these vaccines. Genetically engineered vaccines hold great promise for the future, but the potential of these vaccines to improve human and animal health has yet to be fully realized.
https://www.ncbi.nlm.nih.gov/pubmed/9514708
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Genetically modified viruses: vaccines by design.
2001
https://www.ncbi.nlm.nih.gov/pubmed/11482348
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Genetically Engineered Vaccines
Below are some examples of Genetically Engineered vaccines:
Subunit vaccines: They represent technologies ranging from the chemical purification of components of the pathogen grown in vitro to the use of recombinant DNA techniques to produce a single viral or bacterial protein, such as Hepatitis B surface antigen for example. The disadvantage of such vaccines is that immune responses, especially T-lymphocyte activation, are too weak.
DNA vaccines: They employ genes encoding proteins of pathogens rather than using the proteins themselves, a live replicating vector, or an attenuated version of the pathogen itself. They consist of a bacterial plasmid with a strong viral promoter, the gene of interest, and a polyadenylation/transcriptional termination sequence. The plasmid is grown in bacteria (e. coli), purified, dissolved in a saline solution, and then simply injected into the host. In present versions only very small amounts of antigens are produced within the vaccinated individual.
Recombinant (DNA) vaccines: Made by isolation of DNA fragment(s) coding for the immunogen(s) of an infectious agent/cancer cell, followed by the insertion of the fragment(s) into vector DNA molecules (i.e. plasmids or viruses) which can replicate and conduct protein-expression within bacterial, yeast, insect or mammalian cells. The immunogen(s) may then be completely purified by modern separation techniques. The vaccines tend to give good antibody responses, but weak T-cell activation.
Naked DNA vaccines: They are engineered from general genetic shuttle vectors and constructed to break species barriers. They may persist much longer in the environment than commonly believed. Upon release or escape to the wrong place at the wrong time. Horizontal gene transfer with unpredictable long- and short-term biological and ecological effects is a real hazard with such vaccines. There may be harmful effects due to random insertions of vaccine constructs into cellular genomes in target or non-target species.
Live vector vaccines: These are produced by the insertion of the DNA fragment(s) coding for an immunogen(s) intended for vaccination into the genome of a ‘non-dangerous’ virus or bacterium, the vector. The insertion is performed in such a way that the vector is still infectious ‘live’.
RNA vaccines: This involves the use of in vitro synthesised RNA (a single-stranded relative of DNA). RNA are different from DNA vaccines in that there is no risk of chromosomal integration of foreign genetic material.
Genetically engineered vaccines and their potential risks
Synthetic and recombinant vaccines are produced under contained conditions. Only a polypeptide which may confer protective immunity to a given disease agent are brought out of the production unit and used as vaccine. Such vaccines carry the same advantages and disadvantages as traditional “killed” or “subunit” vaccines. It is conceivable that new vaccine delivery systems and basic knowledge about immune system interactions will make these vaccines more efficient in the near future.Genetically modified viruses and genetically engineered virus-vector vaccines carry significant unpredictability and a number of inherent harmful potentials and hazards. The immunological advantages of such vaccines are related to the fact that the viruses are “live” and infect the vaccinated individuals. It has, however, been demonstrated that minor genetic changes in, or differences between, viruses can result in dramatic changes in host spectrum and disease-causing potentials. For all these vaccines, important questions concerning effects on other species than the targeted one are left unanswered so far. The opportunity of a genetically engineered vaccine virus to engage in genetic recombinations with naturally occurring relatives is another unpredictable option. The new, hybrid virus progenies resulting from such events may have totally unpredictable characteristics with regard to host preferences and disease-causing potentials. Furthermore, when genetically modified or engineered virus particles are broken down in the environment, their nucleic acids will be released, representing the same unpredictable risk potentials as the DNA and RNA vaccines discussed below.
Much basic work is needed before recombinant bacterial vectors may be taken into practical use. For instance, it was recently demonstrated that genetically engineered bacteria might transfer their new gene efficiently to indigenous bacteria in the mammalian gut. This potential risk has not been investigated for bacteria that are now being genetically engineered as oral vaccines.
http://www.dogsnaturallymagazine.com/genetically-engineered-vaccines-2/
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Vector-based genetically modified vaccines: Exploiting Jenner’s legacy
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Hepatitis B vaccine produced cheaply and efficiently using GMO corn
A small technology venture, called Applied Biotechnology Institute (ABI) in San Luis Obispo, CA, a lovely small city along the Central Coast of California, has developed a method to genetically modify corn to produce medically useful proteins. They are focused on specialized enzymes, sweeteners, and vaccines.
According to the World Health Organization, nearly 800,000 individuals die each year worldwide as a result of hepatitis B, an infectious virus that afflicts the liver. A safe and effective vaccine has been available since 1982 and is typically administered via three intramuscular injections over a period of about six months. The vaccine is recommended for babies, children and adults.
ABI has developed a fascinating method to incorporate the antigens for hepatitis B (not the virus itself, just some of the protein antigens that induce an immunological response) by inserting the genes for these proteins into the corn. When the corn matures, the corn’s seed germ–that is, the part of the corn seed that germinates into a new plant, not the other use of the word germ, infectious diseases like bacteria or viruses–is isolated from the rest of the seed.
The hepatitis B antigens are in the seed’s germ, and that is ground up. The antigens are then extracted and processed into a wafer that can be consumed orally to give a booster effect to those who have had the vaccine many years in the past. This wafer is heat resistant, and can be easily and cheaply transported to any number of locations. No syringes. No needles. No refrigerators. It is nearly nirvana for vaccination, at least for hepatitis B.
https://geneticliteracyproject.org/2014/11/24/hepatitis-b-vaccine-produced-cheaply-and-efficiently-using-gmo-corn/
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A Genetically Modified Malaria Vaccine Has Passed an Important Hurdle
Researchers have tested a modified malaria parasite in humans that has been shown to be safe and to trigger an immune response.
January 4, 2017
It seems a primitive way to fight one of the world’s worst diseases, but 10 volunteers have been bitten by malaria-carrying mosquitoes in an effort to test out a new kind of genetically modified vaccine. So far, so good: no one got sick, and all 10 subjects developed antibodies, suggesting the new vaccine was doing its job.
Researchers led by Jim Kublin at the Fred Hutchinson Cancer Research Center in Seattle knocked out three genes in the parasite Plasmodium falciparum, which causes the type of malaria most commonly found in Africa. Previous testing in mice showed that deleting just those genes was enough to prevent the parasite from progressing through its life cycle—infected mice developed antibodies but never got sick. They reported the results of the first trials in people today in the journal Science Translational Medicine.
It’s a promising step for a vaccine that is badly needed—in 2015, the World Health Organization estimated that 214 million people got malaria and 438,000 died from it.
Several malaria vaccines are in development, but they all come with big drawbacks. One, called RTS,S, uses a genetically modified protein from P. falciparum to condition the immune system against the parasites. It has undergone large human trials and it’s scheduled to be rolled out in three countries in sub-Saharan Africa in 2018. But it’s only effective in about a third of patients.
Another method in trials uses radiation to damage parasites’ DNA and weaken them. They are then injected. The vaccine, called PfSPZ, has been shown to provide lasting protection in more than half of people, but only if they went through four rounds of injections.
Irradiation damages DNA at random, whereas targeted gene deletion achieves a consistent result—that’s likely why the volunteers who were bitten all had the same reaction. That makes this most recent vaccine particularly attractive.
But it’s early days. For one thing, volunteers exposed to the parasites with deleted genes were never infected with full-strength bugs to see if their immune systems would fend them off. That crucial next step is due to occur sometime next year, according to Science. If all goes well, the vaccine could then move into large-scale trials.
Until then, a vaccine that helps half, or even a third, of people who get it isn’t ideal, but it would still save thousands of lives. And it would be a welcome addition to other, more prosaic tactics for fighting malaria, like distributing bed nets, that have made progress against the disease.
https://www.technologyreview.com/s/603304/a-genetically-modified-malaria-vaccine-has-passed-an-important-hurdle/
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Are You Concerned Over Genetically Modified Vaccines?
October 02, 2012
Nobody Knows What Happens When You Inject People with GM Vaccines
There have been some fair warnings, though. In 2006, researchers wrote in the Journal of Toxicology and Environmental Health:1
"Genetically modified (GM) viruses and genetically engineered virus-vector vaccines possess significant unpredictability and a number of inherent harmful potential hazards... Horizontal transfer of genes... is well established. New hybrid virus progenies resulting from genetic recombination between genetically engineered vaccine viruses and their naturally occurring relatives may possess totally unpredictable characteristics with regard to host preferences and disease-causing potentials.Though this was six years ago, little has changed even as the technology has advanced. Today we have several different types of GM vaccines in production, development or research phases, such as:
...There is inadequate knowledge to define either the probability of unintended events or the consequences of genetic modifications."
There are experimental GM vaccines being developed that use tumorigenic cancer cells and cells from humans, dogs, monkeys, cows, pigs, rodents, birds and insects. What happens when foreign DNA is inserted into the human body is a mystery. Will it trigger undesirable changes in human cells or tissues? Will it combine or exchange genetic material with human DNA? Will it transfer to future generations? No one knows...
- DNA vaccines: DNA for a microbe's antigens are introduced into the body, with the expectation that your cells will take up that DNA, which then instructs your cells to make antigen molecules. As the National Institute of Allergy and Infectious Disease (a division of the National Institutes of Health) put it, "In other words, the body's own cells become vaccine-making factories."2
- Naked DNA vaccines: A type of DNA vaccine in which microscopic particles coated with DNA are administered directly into your cells.
- Recombinant Vector vaccines: Similar to DNA vaccines, but they use a virus or bacteria to act as a vector (or "carrier) to introduce microbial DNA into your cells.
"We don't know what portion of the [GM] DNA can be incorporated into our own genome, we don't know what portion could be inheritable to our children, we also don't know what happens when the immune system is exposed to DNA that has been recombined in lots of ways that the human body, through the course of time, has never had any exposure to... what diseases of the immune system may occur because of these exposures," Debold said.
"Use of foreign DNA in various forms has the potential to cause a great deal of trouble, not only because there is the potential for it to recombine with our own DNA, there is the potential for it to turn the DNA 'switches,' the epigenetic parts of the DNA, on and off."
Vaccine Adjuvants Used in GM Vaccines May be Even More Toxic Than Usual
An adjuvant is added to a vaccine in order to boost the body's immune reaction to the viral or bacterial antigen contained in a vaccine. Under ideal circumstances, the antigen is what your body responds to and makes antibodies against (e.g. the lab altered viral or bacterial organisms being injected). By boosting your body's immune response in this artificial way, the vaccine manufacturer can use a smaller amount of antigen, which makes production less expensive and the product more profitable (although definitely not safer, as adjuvants are usually foreign substances, metals or chemicals which can cause the immune system to overreact and attack the host body.)
Aluminum is a common vaccine adjuvant and also a well-known neurotoxin that can cause chronic inflammation in the body, including the brain. Although aluminum adjuvants have been added to inactivated vaccines used for decades in the U.S., aluminum-based adjuvants are not strong enough for GM vaccines, according to Debold, so drug companies are primarily interested in using oil-based adjuvants, like squalene, and other substances that can hyper-stimulate the body's immune response.
While oil-based vaccine adjuvants like squalene have been proven to generate powerful acute inflammatory immune responses that stimulate increased production of antibodies, they have also been associated with unresolved, chronic inflammation in the body that can cause brain and immune system dysfunction, including autoimmune diseases.3 While the U.S. Food and Drug Administration (FDA) has so far not licensed any vaccines distributed in the U.S. that contain squalene as an adjuvant, squalene adjuvants are used in some vaccines sold in Europe and other countries.
The Close Ties Between GM Foods and GM Vaccines
The companies that make vaccines and GMOs (genetically modified organisms) are deeply intertwined, only recently spinning off or merging to specialize in one or the other. Most vaccine revenues are earned by five companies that together held nearly 80 percent of the market in 2010:5
These companies, which use genetic engineering to produce vaccines, are also primarily responsible for the introduction of genetic engineering into the food supply. For instance:
- Sanofi Pasteur
- GlaxoSmithKline
- Merck & Co.
- Pfizer
- Novartis
- Genetic engineering giant Syngenta (third in total sales in the commercial agricultural seeds market) is the progeny of parent companies Novartis and AstraZeneca.
- In 2001, Bayer CropScience became a leading genetically engineered crop producer with its purchase of Aventis' agribusiness division.6
- In 2004, Aventis merged with and into Sanofi. The new Sanofi-Aventis Group became the world's 3rd largest pharmaceutical company. Aventis Pasteur, the vaccine division of Sanofi-Aventis Group, changed its name to Sanofi Pasteur. Sanofi Pasteur is the vaccines division of Sanofi Group. It is the largest company in the world devoted entirely to vaccines.
- Prior to splitting its genetically engineered crop business from its vaccine business, Aventis was known primarily for the StarLink corn debacle (a type of GM corn grown for use in animal feed that contaminated the U.S. food supply in 2000). Bayer now sells Aventis's Liberty Link crops, engineered to tolerate high doses of the company's toxic herbicide called Liberty (glufosinate).7
- Stauffer Seeds was a spin-off of Stauffer Chemical, formerly a division of Novartis.8 Stauffer Seeds and Prodigene conducted clinical trials on pigs using an edible vaccine for transmissible gastroenteritis virus (TGEV) expressed in corn.9
- Prodigene was caught contaminating the food supply with its edible vaccine and the company went out of business, but not before it received a $6-million investment from the Governors Biotechnology Partnership, chaired by Iowa Governor Tom Vilsack. Vilsack, now the Obama Administration's USDA Secretary, didn't want any restrictions placed on experimental pharma crops. In reaction to suggestions that pharma crops should be kept away from food crops, Vilsack argued that 'we should not overreact and hamstring this industry.'10
- Prior to 1997, Monsanto (the world leader in GM crops) operated under three parts, the Ag Business (for agricultural products), the Chemicals Business, and the Pharmaceuticals Business, which is now Pharmacia, a subsidiary of Pfizer, the biggest pharmaceutical company in the world and the largest manufacturer of vaccines for food animals.11, 12
- GlaxoSmithKline, while producing few products for food or agriculture, has been genetically engineering plants, animals and microorganisms for use in vaccines, pharmaceuticals and medical research.13
Bill Gates, Warren Buffet Supporting Propagation of Both Vaccines and GMOs
The most influential, and, of course, richest advocates for genetic engineering and vaccines are Bill Gates and Warren Buffet. They have business as well as philanthropic interests in these technologies and their Gates Foundation (Buffet has donated over $1.5 billion to the Foundation) allows them to mix business with philanthropy.
They – and the corporations they invite to join them – use the tax shelter of a non-profit organization to invest in for-profit enterprises. Gates & Buffet get tax write-offs for putting money in their foundation, but their foundation can give money (both as grants & investments) directly to for-profit corporations creating for-profit products.
This, obviously, creates huge conflict of interests.
For instance, Monsanto and other biotech companies have collaborated with the Gates Foundation via the Alliance for a Green Revolution in Africa (AGRA) to promote the use of genetically modified (GM) crops in Africa. The Gates Foundation has donated hundreds of millions of dollars to AGRA, and in 2006 Robert Horsch was hired for the AGRA project. Horsch was a Monsanto executive for 25 years. In a nutshell, the project may be sold under the banner of altruism and 'sustainability,' but in reality it's anything but. It's just a multi-billion dollar enterprise to transform Africa into a GM-crop-friendly continent. The Foundation has also invested heavily in Monsanto stock, purchasing over $23 million worth in 2010.14
The Gates Foundation is also closely partnered with Big Pharma, to whom Bill Gates pledged $10 billion to distribute and administer multiple vaccines to children around the world. This, too, is billed as a humanitarian effort to save lives, but what children living in poverty in developing countries need most is healthy, plentiful food, clean water, better sanitation and improved living conditions. These are the keys to preventing the spread of infectious disease, and they appear to be wholly ignored by Bill Gates, Warren Buffet and non-profit organizations with financial ties to Big Pharma – at the children's expense.
The Gates Foundation is even funding surveillance of anti-vaccine groups, and the following vaccine companies are supported by the Foundation through both investments and philanthropic projects:
- Sanofi
- GlaxoSmithKline
- Merck
- Pfizer
- Novartis
http://articles.mercola.com/sites/articles/archive/2012/10/02/vicky-debold-on-gmo-vaccines.aspx
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A vaccine produced by genetic engineering? Why not!
Hepatitis B is a liver disease caused by HBV (hepatitis B virus). The vaccine developed to prevent infection by this virus consists of little bits of HBV that help the body defend itself against the whole virus. Production of this vaccine consists of producing HBV fragments... a case made to order for microorganisms!
The first hepatitis B vaccine consisted of virus fragments, isolated from sick individuals' blood, likely to be recognized by the body's defences. When administered to healthy people, these fragments allowed to the body to rapidly recognize the entire virus and eliminate it before it could cause infection. But this vaccination technique was not without its hazards. Despite purification procedures, a complete virus sometimes contaminated the vaccine resulting in a healthy person contracting the very disease the vaccine was meant to prevent! In addition, the use of infected individuals as the source of the vaccine presented practical difficulties. The development of a microorganism-based process to produce virus fragments was thus a welcome innovation.
http://www.musee-afrappier.qc.ca/en/index.php?pageid=3115d&page=3115d-engineering-vaccine-e
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An Orphan in Science: Environmental Risks of Genetically Engineered Vaccines
1999
5.2.4.
Genetically modified viruses for homologous immunization
Although gene-deleted viruses were initially considered as a promising strategy,
recent
experience with an AIDS-related vaccine have raised serious concerns
about both target and non-target effects of such vaccines. A vaccine
made by deleting several genes from the Simian immunodeficiency
virus(SIV), supposed
to be safe, caused AIDS in infant and adultmacaques (Baba et al., 1999).
To illustrate some of the theoretical hazards, and the lack of key knowledge
for risk assessments, I have chosen to use gene-deleted Pseudorabies virus vaccine as an example.
Example:
Gene-deleted Pseudorabies vaccine
The alterations
The
vaccine virus was developed by genetic engineering of a live,
attenuated PRV strain. Thevaccine virus is a multi-deletion mutant. The
main deletion (2055 basepairs) is situated in the small unique (Us) part
of the genome. It removes gE and part of the so-called 11K
protein-gene. A 2 basepair deletion has been introduced into the
tk(thymidine kinase) gene, which is hence left non-functional, i.e. the
vaccine PRV has a TK÷
phenotype.
Finally, a deletion has removed 73 basepairs from a non-coding control
region in the repeated sequences. The gE-deletion was selected for after
a targeted, homologous recombination in cell culture, while the small
tk gene deletion was achieved by genetic engineering.
The effects.
gE
seems to be an essential protein in transneuronal spread of PRV.
Studies have shown that gE-negative PRV replicates in peripheral
tissues, infects first-order
neurons and spreads towards the CNS via both the olfactory and trigeminal routes (review by Mulder et al., 1997).
The second-and third-order neurons in the porcine CNS do, however, seem
to
be less efficiently infected by a gE-negative than a wild-type PRV
(Jacobs, 1994; Kritas et al., 1995). But there may be considerable
differences in the effect of gE-deletions between different neuron
circuits in the pig, and also between pigs and other permissive animal
species (Cord et al., 1992; ter Horst et al., 1993; Whealy et al., 1993;
Standish et al., 1994). The PRV-encoded thymidine kinase (TK) is not
considered essential for growth in dividing cells, but seem to be
required for productive infection of non-dividing cells such as neurons
and
resting peripheral blood mononuclear cells (Mulder et al., 1995 and 1997).
Several types of PRV vaccines are available (Kimman et al., 1995; Mulder et al, 1997):
•Killed whole virus vaccines
•Subunit vaccines
•Conventionally attenuated live vaccines
•Genetically engineered live vaccines
In
general, live vaccines are more potent than the dead ones, especially
in mounting an efficient cellular immune response. Conventionally
attenuated live vaccine PRV strains have, however, been shown to contain
mutations that may reduce immunogenicity. There is also the risk that
such PRV strains may revert to full virulence. These were the main,
expressed reasons why several research groups started to develop
genetically engineered vaccine viruses with defined
deletions
in the genes encoding glycoproteins E (gE, formerly gI), C (gC) or G
(gG) (Kit et al., 1987; Marchioli et al., 1987; Moorman et al., 1990).
In order to enhance their safety, TK which enhances viral DNA
replication has been deleted from these engineered PRV strains (Mulder
et al., 1997).
In
eradication campaigns, gE-deleted vaccine strains have been used in
conjunction with a serological test that specifically detects antibodies
against gE. This makes it possible to identify vaccinated individuals
that have become infected with wild-type PRV (vanOirschot et al.,1990;
Kit, 1990).
Concerns connected to genetically engineered PRV vaccines
PRV transmission
So
far short-term economical interests have governed vaccine development,
i.e. to avoid losses in slaughter weight have been more important than
eradication of PRV. The vaccines that have been introduced so far,
including the gE-/tk-deleted vaccine, are not able to stop transmission
of wildtype PRV strains, and the vaccine strain itself may spread to
contact pigs, setting the stage for single- or multi-step
recombinational events (Bouma et al., 1997b; Parker et al., 1997;
Bouma
et al., 1997a; de Smet et al., 1992; Mulder et al., 1995). Gene-deleted
pseudorabies vaccines have now been shown able to infect sheep (see
below).
5.2.5. Live virus-vector vaccines
Example: Recombinant, live VV/rabies vaccine
General risk factors connected with release of live recombinant VV/rabies vaccine
Most
potential hazards may be categorized under the heading «non-target
effects ». These may be due to the released virus itself. The
innocuousness of VV/rabies recombinants is by no means proven (Hanlon
et al., 1997; Zhen et al., 1996; Moos, 1995). But equally possible is
the emergence of new virus strains as a result of recombination events
between vaccine virus and a naturally occurring relative taking place in
a double-infected individual. Such hybrid viruses
may have totally unpredictable characteristics with regard to host species
susceptibility and virulence in different animal species.
If
a genetically modified orthopoxvirus infects an individual, animal or
human, which already carries another orthopoxvirus, a hybrid progeny
virus with
unpredictable
pathogenicity and altered host range might be the outcome. Such worst
case scenarios include new emerging diseases and ecological
catastrophes. In our lab we have shown that some orthopoxviruses
circulate among small
rodents in Norway.
In
addition, Norwegian orthopoxvirus strains, isolated from a clinically
ill house-cat and a woman, show genetic characteristics in common with
both vaccinia
virus and cowpox virus. It is important to gain knowledge about the
biological
and genetic diversity among the circulating Norwegian orthopoxviruses,
as well as about their ecology and reservoir species.
It
is also important to verify whether the two Norwegian isolates
represent hybrid viruses due to recombination events. Finally, one needs
to investigate the recombination potential between naturally occurring-
and genetically modified
orthopoxvirus
in authentic Norwegian host animals. For VV there is little relevant
knowledge concerning these mechanisms (Moss, 1996; Fenner,
1996).
Some experts have voiced the opinion that there is a number of
questions that have to be answered in a satisfactory way before any
virus should be released:
https://www.biosafety-info.net/file_dir/2448448883bd316e50.pdf
---------------------------------------------
Genetically Engineered Virus-Vectored Vaccines – Environmental Risk
Assessment and Management Challenges
4.3 Ecological distribution of Poxviridae
The family Poxviridae contains a vast number of divergent viruses with equally divergent
host specificities and host ranges. It seems justified to state that poxviruses will be found
in any vertebrate species and geographical area where they are systematically looked for.
However, very little is known about the characteristics and geographical distribution of
most poxviruses occurring naturally in the field. This knowledge would be necessary if a
meaningful risk assessment of poxvirus-based GM vaccines use or release in any target area
should be undertaken. Without such knowledge there is no possibility of making
meaningful ERA inclusions of adverse effects due to recombination events between GM
poxvirus vaccines and naturally occurring poxviruses circulating within the actual area for
application. Many poxviruses are capable of zoonotically infecting man. It is likely that variola virus is derived from an ancient zoonotic virus that originated from a now extinct animal host species.
http://cdn.intechopen.com/pdfs/25756.pdf
-------------------------------------
Anti-GMO But Pro-Vaccine?
GMOs in Vaccines?
And
then there is a topic few on the non-GMO side seem to want to tackle
head on: the present-day vaccine schedule contains a wide range of
genetically modified ingredients that are being injected directly into
the most vulnerable infants and children in our population. The HPV vaccine,
for instance, which is comprised of a genetically modified form of
yeast containing HPV-like antigens, has the worst adverse reports events
(some lethal) on record. Mind you, it has never once been proven to
prevent a single case of cervical cancer because proxy markers for
efficacy and not clinical proof were all the FDA required for its
approval. Where is the non-GMO uproar about this? How do the most
vulnerable and victimized populations benefit from the non-GMO and
non-vaccine movements turning away from one another, or claiming that
the explosion of autism diagnoses is only caused by either GMO foods or
vaccines, not both, which is the more obvious likelihood?
Even
the rage against Monsanto, widely considered "the world's most evil
corporation," may constitute a diversionary tactic against the
increasing number of activists in need of a suitable, socially
sanctioned object of catharsis. Millions marched against Monsanto, but
how many were aware that Monsanto is owned by an even larger
corporation, Pfizer, who has been expanding its vaccine portfolio and influence on vaccine legislation while the public eye has been largely focused on labeling GMOs. [1]:
Former Monsanto is today known as Pharmacia LLC. Pharmacia is now a wholly owned subsidiary of Pfizer Inc., which operates the Pharmaceutical business." [Source: Monsanto: Who We Are]
But
you can't just blame one or two companies for the increasingly bleak
picture. The direction of technological science, unguided by internal
ethical principles or external regulatory controls, is towards the
complete convergence of the vaccine and GMO agendas in a way that
precludes informed consent and any vestige of consumer/patient choice.
This abstract provides insight into what I mean:
2014 May 1.
Stable accumulation of seed storage proteins containing vaccine peptides in transgenic soybean seeds.
Abstract
There has been a significant increase in the use of transgenic plants for the large-scale production of pharmaceuticals and industrial proteins. Here, we report the stable accumulation of seed storage proteins containing disease vaccine peptides in transgenic soybean seeds. To synthesize vaccine peptides in soybean seeds, we used seed storage proteins as a carrier and a soybean breeding line lacking major seed storage proteins as a host.Vaccine peptides were inserted into the flexible disordered regions in the A1aB1b subunit three-dimensional structure. The A1aB1b subunit containing vaccine peptides in the disordered regions were sorted to the protein storage vacuoles where vaccine peptides are partially cleaved by proteases. In contrast, the endoplasmic reticulum (ER)-retention type of the A1aB1b subunit containing vaccine peptides accumulated in compartments that originated from the ER as an intact pro-form. These results indicate that the ER may be an organelle suitable for the stable accumulation of bioactive peptides using seed storage proteins as carriers.
http://www.greenmedinfo.com/blog/anti-gmo-pro-vaccine
-----------------------------------------
New, Genetically-Modified HIV Vaccine Enters Next Phase of Trials
Dec 1, 2016
Early trials showed the vaccine caused an immune response, but whether that’s enough to prevent someone from getting HIV is another question.
A new possible HIV vaccine that uses a genetically-modified version of the virus is heading into Phase II trials. The results of an earlier trial showed it provoked an immune response against the virus, but now we need to see if that response is strong enough to protect someone from getting infected with HIV in the first place.
The vaccine uses an inactivated—or "dead"—version of the HIV virus, the first vaccine of this type for HIV. Previously, inactivated vaccines haven't been attempted for HIV because it was considered too risky, but this candidate uses a version of the virus that has been genetically modified—swapping out some of the genes with genetic material from honey bees—to make it less virulent and easier to mass produce.
https://motherboard.vice.com/en_us/article/xygmaq/new-genetically-modified-hiv-vaccine-enters-phase-ii-trials
-------------------------------------
A new malaria vaccine just passed a critical milestone using GM parasites
In recent years, the world has recorded tremendous progress in the fight against malaria. The World Malaria Report 2015 shows malaria mortality rates have fallen by 66% among all age groups and by 71% among children under five in Africa since 2000. But there is still work to do and a new vaccine developed by a US-based team is showing promising results, and could accelerate progress.
Creating the vaccine, researchers infected people with weakened, genetically modified forms of the Plasmodium falciparum parasite in safety trials. The weakened malaria parasite, while unable to complete its lifecycle and develop into full-blown malaria, exposes the immune system to the disease and stimulates a response that could block an actual infection.
After tests on ten people during trials, the results were encouraging: there were no cases of full malaria infection and no significant side-effects.
https://qz.com/878741/a-malaria-vaccine-using-genetically-modified-parasites-is-showing-promising-results/
------------------------------
Tobacco is genetically engineered to produce vaccine
09/02/07
Scientists have genetically engineered tobacco plants to produce a protein for a vaccine against amoebiasis — a disease predominantly affecting Central and South America, Africa and Asia.
The World Health Organization estimates that amoebiasis, caused by the parasite Entamoeba hisolytica, causes 50 million cases and 100,000 deaths a year. There is currently no approved vaccine against the disease.
According to the researchers, the method used achieves high production levels at a low cost, and also prevents modified genes from crossing to other plants in the environment.
Henry Daniell and colleagues at the University of Central Florida in the United States added the gene for a molecule that prompts an immune response in humans — the antigen — to the chloroplasts of the tobacco plant. Chloroplasts are the parts of the cells which contain the pigment that makes leaves green.
With this method, the gene is not carried in the plant's pollen and so cannot migrate to other plants.
The tobacco-derived antigen successfully prompted an immune response in animal tests that was 4–20 times higher than that from other engineered antigens.
The researchers calculate that an average yield of 24 milligrams of vaccine antigen per plant could produce 29 million doses of vaccine per acre of the transgenic crop.
The researchers say future development should be directed toward using carrot or lettuce plants, paving the way for a cheap oral vaccine. Tobacco was used initially, as it is easy to engineer in the lab.
http://www.scidev.net/global/gm/news/tobacco-is-genetically-engineered-to-produce-vacci.html
-------------------------------------------------
Genetically Engineered Plants as a Source of Vaccines Against Wide Spread Diseases
2014
Genetically Engineered Plants as a Source of Vaccines Against Wide Spread Diseases: An Integrated View provides an integrated outlook of the disciplines involved in the development of plant-based vaccines as well as an updated compilation of the successful developments in the field. The volume covers immunological aspects of mucosal vaccine design, molecular approaches to attain high levels of the recombinant antigens, the rationale of using bioreactor to expand plant biomass,
http://www.springer.com/us/book/9781493908493
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Bacteria threaten to kill America’s $3-billion orange industry—so scientists are weaponizing viruses to fight it
May 17, 2017
The culprit bacteria belongs to the genus Liberibacter, and it causes citrus trees to have bitter, misshaped fruits with green lower halves. Since its arrival in the US in 2005, it has spread very quickly, thanks to the flying insect called Asian citrus psyllids (Diaphorina citri). If it isn’t stopped, it could end production of citrus fruits in the US.
The savior could be citrus tristeza virus, which—without being engineered—is actually known to cause slow death of citrus trees. The engineered version, however, is benign to the fruit and goes after Liberibacter instead.
The idea of deploying engineered viruses comes courtesy of the Southern Garden Citrus, a producer of oranges in Florida. Field trials are underway, and now the company is seeking the approval of the US Food and Drug Administration (FDA) for commercial use. The approval process may take two years.
The period of public comment ended last week, and the FDA will now be assessing the environmental impact of the engineered virus. Because the virus doesn’t affect the fruit, Southern Garden Citrus can argue that the plants using it won’t produce genetically modified (GM) oranges. This way the company could sidestep regulations and public outcry usually attached to GM crops.
“There’s a real race on right now to try to save the citrus,” Carolyn Slupsky, a food scientist at the University of California at Davis, told Nature. “This disease is everywhere, and it’s horrible.”
The engineered virus isn’t the only weapon against Liberibacter. Scientists are also trying to genetically engineer the crop using a technique called CRISPR to create varieties that are resistant to the bacterial disease. Others are using another technique called RNA interference to disrupt the genes that allow the insects to spread the bacteria.
https://qz.com/985557/genetically-engineered-viruses-might-be-the-only-way-to-stop-bacteria-killing-3-billion-orange-industry-in-the-us/
-----------------------
Scientists Dish Up Rice Vaccine to Fight Cholera
June 11, 2007
Cholera toxin implanted in rice provokes strong immunity in mice while surviving the rigors of acid digestion
https://www.scientificamerican.com/article/scientists-dish-up-rice-vaccine-to-fight-cholera/
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Genetically modified rice as medicine
2013
A genetically modified strain of rice might one day be used to prevent life-threatening rotavirus infections among children in the developing world, according to researchers.A study published Thursday in the Journal of Clinical Investigation concluded that genetically modified rice seeds helped to prevent and treat rotavirus-induced diarrhea in young lab mice.
http://www.latimes.com/science/sciencenow/la-sci-sn-genetically-modified-rice-as-medicine-20130807-story.html
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Banana Vaccines: A Conversation with Dr. Charles Arntzen
http://www.jyi.org/issue/banana-vaccines-a-conversation-with-dr-charles-arntzen/
----------------------------
Edible Vaccines
https://www.mcdb.ucla.edu/Research/Goldberg/HC70A_W12/pdf/EdibleVaccines.pdf
------------------------------
Experimental Vaccines
Genetically Modified Organism’s
http://experimentalvaccines.org/big-pharma/genetically-modified-organisms/
-----------------------------------
Genetically engineered vaccines for control of Aujeszky's disease (pseudorabies).
1990
http://europepmc.org/abstract/med/2174594
------------------------------------
Immune Efficacy of a Genetically Engineered Vaccine against Lymphocystis Disease Virus: Analysis of Different Immunization Strategies
2011
https://www.hindawi.com/journals/ecam/2011/729216/
-----------------------
Vaccination with genetically engineered allergens prevents progression of allergic disease
- In a clinical trial, we developed and evaluated a type of
allergen-specific immunotherapy that is based on genetically engineered
allergens with reduced allergenic activity.
The vaccines are based on genetically engineered derivatives of Bet v 1,
the main
elicitor of birch pollen allergy, which is
one of the most common allergies affecting >100 million individuals
worldwide (10).
We produced two recombinant Bet v 1 (rBet v 1) fragments and a rBet v 1
trimer, both of which are preparations with a >100-fold
reduced allergenic activity compared with the
wild-type Bet v 1 allergen (11, 12). The hypoallergenic rBet v 1 derivatives were adsorbed to aluminium hydroxide, as used in standard vaccination protocols.
We included 124 birch pollen-allergic patients, whose reactivity to the major allergen of birch, Bet v 1, was confirmed by
component-resolved allergy diagnosis (13), in a double-blind, placebo-controlled study that was performed at three study centers. We report a detailed analysis of
the immunological mechanisms underlying vaccination with genetically modified allergens...
-----------------------
Genetically modified enterotoxigenic Escherichia coli vaccines induce mucosal immune responses without inflammation
2007
Abstract
Objective: Enterotoxigenic Escherichia coli
(ETEC) is a major cause of acute diarrhoea in children in the
developing world, in travellers and in the military. Safe, effective
vaccines could reduce morbidity and mortality. As immunity to ETEC is
strain specific, the ability to create vaccines in vitro which express
multiple antigens would be desirable. It was hypothesised that three
genetically attenuated ETEC strains, one with a genetic addition, would
be immunogenic and safe, and they were evaluated in the first licensed
UK release of genetically modified oral vaccines.
Methods:
Phase 1 studies of safety and immunogenicity were carried out at a
Teaching Hospital in London. Varying oral doses of any of three oral
vaccines, or placebo, were administered to volunteers of both sexes
(n = 98). Peripheral blood responses were measured as serum antibodies
(IgG or IgA) by ELISA, antibody-secreting cell (ASC) responses by
enzyme-linked immunospot (ELISPOT), and antibody in lymphocyte
supernatant (ALS) by ELISA. Mucosal antibody secretion was measured by
ELISA for specific IgG and IgA in whole gut lavage fluids (WGLFs).
Results:
Significant mucosal IgA responses were obtained to colonisation factors
CFA/I, CS1, CS2 and CS3, both when naturally expressed and when
genetically inserted. Dose–response relationships were most clearly
evident in the mucosal IgA in WGLF. Vaccines were well tolerated and did
not elicit interleukin (IL) 8 or IL6 secretion in WGLF.
Conclusions:
Genetically modified ETEC vaccines are safe and induce significant
mucosal IgA responses to important colonisation factors. Mucosal IgA
responses were clearly seen in WGLF, which is useful for evaluating oral
vaccines.
http://gut.bmj.com/content/56/11/1550
------------------
PaxVax applies to have GM cholera vaccine tested in Australia
Posted
United States-based vaccine company PaxVax is running a trial of the oral vaccine, and has applied to the Federal Government to run part of the trial in Queensland, South Australia, Victoria and Western Australia.
PaxVax says the single-dose vaccine has been genetically-modified to remove the part of the cholera bacteria that makes people sick.
It wants to use Australia as a trial site to test the vaccine on about 1,000 adults, and potentially children, planning to travel overseas to cholera-affected areas.
http://www.abc.net.au/news/2013-12-23/us-company-applies-to-test-gm-cholera-vaccine-in-australia/5172092
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Your Children to be Used as Guinea Pigs for New GMO Vaccine
A US vaccine company has applied for permission to infect up to one thousand individuals, including children, with genetically modified live bacteria contained in an oral vaccine. PaxVax recently submitted their application for permission to begin the first of three international clinical trials for the new oral cholera vaccine “via oral ingestion of the GMOs”. [1]
PaxVax claims to have several safeguards in place to “restrict the spread and persistence of the GMOs and their introduced genetic material” during this clinical trial. That precaution itself should lead any parent to question the wisdom of administering genetically modified live bacteria to their child.
https://vactruth.com/2013/12/06/gmo-vaccines/
--------------------------
FDA Approves First GMO Flu Vaccine: Expected On Market in 2014
http://www.collective-evolution.com/2013/07/12/fda-approves-first-gmo-flu-vaccine-expected-on-market-in-2014/
-----------------------------
FDA approves first GMO Flu Vaccine containing Reprogrammed Insect Virus
Global Research, June 07, 2013
A new vaccine for influenza has hit the
market, and it is the first ever to contain genetically-modified (GM)
proteins derived from insect cells. According to reports, the U.S. Food
and Drug Administration (FDA) recently approved the vaccine, known as
Flublok, which contains recombinant DNA technology and an insect virus
known as baculovirus that is purported to help facilitate the more rapid
production of vaccines.
According to Flublok’s package insert,
the vaccine is trivalent, which means it contains GM proteins from three
different flu strains. The vaccine’s manufacturer, Protein Sciences
Corporation (PSC), explains that Flublok is produced by extracting cells
from the fall armyworm, a type of caterpillar, and genetically altering
them to produce large amounts of hemagglutinin, a flu virus protein
that enables the flu virus itself to enter the body quickly.
http://www.globalresearch.ca/fda-approves-first-gmo-flu-vaccine-containing-reprogrammed-insect-virus/5338052
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Release of Genetically Modified Viruses
1991
INTRODUCTION
At present, viruses are being developed by genetic modifi-
cation for environmental release for two purposes: as
improved viral insecticides and as vaccines. For this review,
genetically modified viruses are defined as those which have
not simply been altered by genetic engineering, for example
by gene deletion, but those which have had (or will have)
heterologous genes introduced into their genome: trans-
genic viruses. Of the few releases that there have been to
date, most have involved baculoviruses and poxviruses and
only these two groups will be discussed here.
-----------------------
FDA approves first GMO flu vaccine containing reprogrammed insect virus
February 08, 2013
(NaturalNews) A new vaccine for influenza has hit the market, and it is the first ever to contain genetically-modified (GM) proteins derived from insect cells. According to reports, the U.S. Food and Drug Administration (FDA) recently approved the vaccine, known as Flublok, which contains recombinant DNA technology and an insect virus known as baculovirus that is purported to help facilitate the more rapid production of vaccines.
http://www.naturalnews.com/039013_flu_vaccine_insect_virus_GMOs.html
------------------------------------------------
Genetically Modified Flu Vaccine: Flublok
Flublok, a new influenza vaccine, has been approved by the United States Food and Drug Administration (FDA). This is the first vaccine to include three different influenza viruses, and also unique in another way – it’s a genetically-modified vaccine.
In clinical trials, Flublok was 44.6 percent effective against all strains of influenza, not just the three in the vaccine. So how is this GMO vaccine made, and is it safe?
Flublok Ingredients
The good news is that Flublok does not contain any preservatives like thimerosal, is safe for those with egg allergies, and doesn’t use a live influenza virus. The ingredients are few and pretty straightforward. The vaccine contains proteins from three influenza viruses. The vaccine is grown in cultures that contain lipids, salts, minerals, vitamins, and amino acids. Proteins from the three influenza viruses are expressed in insect cells, and then extracted, blended and added to single-dose vials. The vaccines for the 2012-2013 season contain influenza A viruses H1N1 and H3N2, and an influenza B virus.A single dose (0.5 mg) of this vaccine contains sodium chloride, monobasic phosphate, and dibasic sodium phosphate; which are all salts. The vaccine also contains polysorbate 20, an emulsifier that keeps the vaccine ingredients from separating, and residual amounts of Triton X-100 and protein from the insect cell. Triton X-100 is a chemical used in the manufacturing process to prevent the ingredients from clumping together.
New Flu Vaccine: How it Works
This newly approved vaccine is grown using insect cells instead of a chicken egg like the standard vaccine. Protein Sciences Corporation has been working on this technique for five years, using a patented technology called the Baculovirus Expression Vector System (BEVS) Platform. This vaccine is made with genetically modified hemagglutinin antigen proteins of the influenza viruses. The hemagglutinin gene is injected into cells from the fall armyworm, which are grown in a culture to produce the protein.https://www.decodedscience.org/genetically-modified-flu-vaccine-flublok/25719
---------------------------------------------
Genetically modified mosquitos could be used to spread vaccine for malaria
A genetically engineered mosquito that vaccinates as it bites has been developed by scientists.
Experts believe "flying vaccinators" could eventually be a radical new way of tackling malaria.
The new approach targets the salivary gland of the Anopheles mosquito.
Scientists in Japan have engineered an insect producing a natural
vaccine protein in its saliva which is injected into the bloodstream
when it bites.
The "prototype"
mosquito carries a vaccine against Leishmania, another potentially fatal
parasite disease spread by sand flies.
Leishmania infection can cause painful sores, fever and weight loss and if untreated may destroy the liver and spleen.
Mice bitten by the vaccinating insect generated antibodies against the Leishmania organism, indicating immunisation.
http://www.telegraph.co.uk/news/health/news/7473887/Genetically-modified-mosquitos-could-be-used-to-spread-vaccine-for-malaria.html
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After Mosquitos, Moths Are the Next Target For Genetic Engineering
By Nathaniel Scharping | May 10, 2017
Though genetically modified crops may steal the spotlight, similarly reprogrammed insects may have just as big an effect on the agricultural industry.
Biotechnology company Oxitec is moving forward with plans to develop genetically engineered diamondback moths in an attempt to reduce populations of the invasive crop pest. Their plan is to release males that will pass on a gene preventing female offspring from reaching maturity and reproducing, which they say will eventually eradicate the moths in North America. Tests have so far been positive, although there are still worries about the prospect of releasing genetically modified organisms into the wild.
http://blogs.discovermagazine.com/d-brief/2017/05/10/genetic-engineering-moths/#.WY-cG-mQzIU
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Genetically modified organisms (GMOs)
While earlier sections of the document have been silent on the subject of GMOs, the laboratory use of genetic modification is an important tool in microbiological research. Furthermore, the generation of genetically modified products under appropriate safety protocols is increasing in many parts of the world and could be beneficial to the UK. The following are examples.
- Genetically modified microbes can be used as vaccines to protect
against infectious diseases of animals, including man. Pathogens that
have been weakened by alteration of their genetic material, or microbes
that do not cause disease, can be engineered to produce foreign antigens
to stimulate host defences without causing harm. Many such vaccines
have now been licensed or approved, and more are being developed. For
example, a recombinant vaccinia virus has been used in Europe to
vaccinate foxes against rabies, and a herpesvirus of turkey (HVT), used
as a vaccine to protect chickens against Marek’s disease, has been
engineered to produce antigens from Newcastle disease (ND) virus and
infectious laryngotracheitis (ILT) virus and is being used to protect
poultry against all three agents in a single dose. This technology can
now be applied to develop genetically modified microbes that induce
greater protection, longer-lasting immunity, or which are more stable or
cross-reactive.
- Genetically modified microbes can be harnessed as sources of useful molecules, including enzymes to aid energy retention from animal diets and insecticidal toxins to control crop pests and vectors of animal disease. Knowledge of pathogen biology can also be used to engineer animals or crops that resist disease. For example, transgenic chickens were recently described that fail to transmit avian influenza owing to expression of decoy RNAs that sequester viral proteins [27], and a similar strategy can be used in crops to instil resistance to plant viruses [28].
https://www.microbiologysociety.org/food-safety-security/genetically-modified-organisms-gmos.html
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Advantages and Disadvantages of Genetically Engineered Vaccines
Abstract
In the modern world, we prefer precision in every walk of life, similarly vaccines with precise description regarding their genetic, phenotypic and antigenic makeup are the choice. Although biology is too precise to be described in simple words still we say nothing is precise in biology, rather we should say nothing is stable and definite in biology but our experience in molecular biology force us to accept that nothing is at stagnation in life and every change is much more precise than we can understand. Before going into details of genetically engineered live vaccines we need to understand the difference between live and killed vaccines are summarized here.
Table 2. Some important antigens expressed in Salmonella mutants (CVD908 aroC-aroD-
mutant of S. e. Typhi, aroA−, dam−aroA− and dam−phoP− mutants of S. e.
Typhimurium etc.)
Diseases/ Pathogens Antigens expressed in/ carried on Salmonella [References]
Eukaryotic pathogens
Eimeria tenella Antigen 5401
Plasmodium falciperum Circum sporozoite protein (CSP)
Onchocerca volvulus Gutathione S-transferase
Echinococcus granulosus Surface antigen
Leishmania mexicana protein gp63
Plasmodium berghei Merozoite Surface Protein-1
Leishmania major T-cell epitope and gp63
Schistosoma haematobium glutathione S-transferase
Bacteria
Anthrax (Bacillus anthracis) Protective antigen (PA)
Pertussis (Bordetella pertussis) P-69, FHA, PTX51
Tetanus (Clostridium tetani) Tetanus toxin fraction C
Pneumococcal infections Alpha-helical region of PspA (pneumococcal surface
(Pneumococcus species) protein A
Streptococcus mutans Saliva-binding region (SBR)
Escherichia coli LTB, CFA1, Ki capsule, K88,
Neiseria meningitidis 28 kDa outer membrane protein (OMP)
Tularemia (Francicella tularensis) 17 kDa OMP
Cholera (Vibrio cholerae) Cholera toxin B subunit (CTB), O antigen
Yersinia spp. Invasin, F1 capsular antigen, invasin
Typhoid (S. Typhi) Vi antigen
Leprosy (Mycobacterium leprae) Many antigens (85A, EAST6, Pst 3, Hsp 65)
Mycobacterium bovis 30-kDa antigen
Dysentery (Shigella sp.) Many ‘O’ antigens
Diphtheria (Corynebacterium Diphtheria toxin
diphtheriae) HpaA and UreB
Viruses
Rabies Glycoprotein
Herpes simplex virus Glycoprotein D
Melanoma virus NYESO-1 antigen, melanoma differentiation antigens
Gp100
Gp100
Hepatitis B virus
Measles virus HBs antigen
Human papillomavirus HA antigen
Influenza A virus Type 16 E7 epitopes]
Simian papilloma virus Nucleoprotein
Transmissible gastroenteritis virus SIV capsid antigen
Murine fibrosarcoma virus Coronavirus S protein
FMD Virus Murine fibrosarcoma antigen
Human immunodeficiency Virus HIV-1 gag and HIV env antigen,
https://www.researchgate.net/publication
/264165830_Advantages_and_Disadvantages_of_Genetically_Engineered_Vaccines
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Debunking One of The Greatest Myths About GE Crops: “Genetic Modification of Crops Has Been Happening In Nature For Thousands of Years”
August 17, 2017http://www.collective-evolution.com/2017/08/17/debunking-one-of-the-greatest-myths-about-ge-crops-genetic-modification-of-crops-has-been-happening-in-nature-for-thousands-of-years/
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Genetically modified tumour vaccines – where we are today
1998
Tumour vaccines are based on weakly immunogenic specific tumour antigens admixed with adjutants in order to elicit, restore or augment antitumour immune responses against residual or metastatic tumour cells. Cellular cytotoxicity is considered to play a major role in eliminating tumour cells. Activation of cellular toxicity requires at least three synergistic signals: presentation of specific tumour antigen, constimulatory signal (B7 molecules) and propagation signal (cytokines) Recently several HLA-restricted specific tumour antigens recognized by cytotoxic T-cells have been characterized. Antibody defined antigens, heat shock proteins and viral antigens are also discussed. First generation vaccines made of whole cancer cells or tumour-cell lysates together with non-specific adjutants produced about 20% of clinical responses and are currently tested in prospective clinical trials. Novel second generation of tumour vaccines employ genetically modified tumour cells, antigen presenting cells (dendritic cells) or recombinant tumour antigens. Tumour cells are modified with genes encoding molecules providing signals for cytotoxic T-cells required for recognition and killing of cancer cells such as B7 constimulatory molecules, HLA proteins and genes of different cytokines. Dendritic cells are modified with genes of specific tumour antigens in order to activate both helper and cytotoxic T-cells. Novel vaccines produce specific immune responses and objective clinical responses with minimal toxicity in phase I/II trials. Advances in gene transfer technology, tumour immunology and better methods of monitoring specific antitumour immune responses allow the hope that tumour vaccines will be introduced into the clinic, at least in some malignancies resistant to systemic therapy so far such as melanoma and renal cell carcinoma.
http://www.cancertreatmentreviews.com/article/S0305-7372(98)90104-9/fulltext
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Mucosal Vaccination and Therapy with Genetically Modified Lactic Acid Bacteria
2011
Lactic acid bacteria (LAB) have proved to be effective mucosal delivery vehicles that overcome the problem of delivering functional proteins to the mucosal tissues. By the intranasal route, both live and killed LAB vaccine strains have been shown to elicit mucosal and systemic immune responses that afford protection against infectious challenges. To be effective via oral administration, frequent dosing over several weeks is required but new targeting and adjuvant strategies have clearly demonstrated the potential to increase the immunogenicity and protective immunity of LAB vaccines. Oral administration of Lactococcus lactis has been shown to induce antigen-specific oral tolerance (OT) to secreted recombinant antigens. LAB delivery is more efficient at inducing OT than the purified antigen, thus avoiding the need for purification of large quantities of antigen. This approach holds promise for new therapeutic interventions in allergies and antigen-induced autoimmune diseases. Several clinical and research reports demonstrate considerable progress in the application of genetically modified L. lactis for the treatment of inflammatory bowel disease (IBD). New medical targets are on the horizon, and the approval by several health authorities and biosafety committees of a containment system for a genetically modified L. lactis that secretes Il-10 should pave the way for new LAB delivery applications in the future.
http://www.annualreviews.org/doi/abs/10.1146/annurev-food-022510-133640
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Mucosal targeting of therapeutic molecules using genetically modified lactic acid bacteria: an update
01 July 2013
Abstract
Lactic acid bacteria (LAB) represent a heterogeneous group of microorganisms naturally present in many foods and those have proved to be effective mucosal delivery vectors. Moreover, some specific strains of LAB exert beneficial properties (known as probiotic effect) on both human and animal health. Although probiotic effects are strain-specific traits, it is theoretically possible, using genetic engineering techniques, to design strains that can exert a variety of beneficial properties. During the two past decades, a large variety of therapeutic molecules has been successfully expressed in LAB, and although this field has been largely reviewed in recent years, approximately 20 new publications appear each year. Thus, the aim of this minireview is not to extensively assess the entire literature but to update progress made within the last 2 years regarding the use of the model LAB Lactococcus lactis and certain species of lactobacilli as live recombinant vectors for the development of new safe mucosal vaccines.
Https://academic.oup.com/femsle/article/344/1/1/552126
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The Disgusting Cow Stuff in Vaccines
8/3/2017
Cow Parts Used for Vaccines
Why are cow parts used for cell cultures and to make vaccines? According to the FDA, “simply because cows are very large animals, commonly used for food, and thus much material is available.“
Wow. Really?
The FDA and other sources go on to report that parts of the slaughtered cows used to make vaccines include lactose and casein from cow’s milk, glycerol from the tallow (fat), gelatin derived from connective tissue, bones, and tendons, and galactose from circulating red blood cells. The milk proteins and gelatin are considered to be stabilizers and are used to “prevent viral degradation.” Allergies to these ingredients have been reported after vaccination. (examples: here and here)
Calf serum is the liquid portion of a blood that remains after red blood cells, white blood cells, proteins, platelets and clotting factors are removed. Albumin is the most abundant type of protein in bovine serum.
Various classifications of bovine serum are used, based on the animal’s age at the time of collection. This is important because the age of the animal impacts the characteristics of the serum. Industry standards classify the serum according to the following guidelines:
| Description by Age | Age |
| Fetal bovine serum | Intra-uterine fetus |
| Newborn calf serum | Less than 21 days old |
| Formula-fed calf serum | Less than 22 weeks old |
| Calf bovine serum | 21 days to 12 months old |
| Adult bovine serum | 12 months or older |
Fetal Bovine Serum
Fetal bovine serum (FBS) is the most widely used, the most difficult to obtain and the most expensive cell culture promoter used in drug manufacturing. In my opinion, FBS is also the most disgusting and disturbing type of serum used in vaccines. Serum is a complex mixture of biological constituents and, even after 50 years of laboratory use, the majority of the bovine subunits have not been fully identified. The age, sex, genetics, quality of food consumed by the mother, and the maturity of the extracted cells creates an enormous amount of lot-to-lot inconsistency. In fact, serum and albumin can be essentially “designed” to meet the needs of a customer by feeding baby calves specialized formula diets.Essentially this means every batch is a completely new experiment. According to a 2014 article on ResearchGate.com:
A recent analysis revealed that FBS contains approximately 1,800 different proteins and more than 4,000 metabolites. The proportions of each vary between different batches and vary by the health of the animal from which it is harvested. FBS has been known to contain adverse factors, like endotoxins, mycoplasma, viral contaminants and even prions that can lead to bovine spongiform encephalopathy, or BSE.BSE is a progressive neurological disorder of cattle commonly called ‘mad cow disease.’ When the FDA was asked, “Do all bovine materials have the same risk of transmitting the BSE agent?”, this was their response:
http://vaxxter.com/the_disgusting_cow_stuff_in_vaccines/
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The Disgusting Stuff in Those SPF-Eggs Used for Vaccines
Extra Viruses in the Egg-based Vaccines
The truth is, each egg is a new experiment.Disturbingly, there is a wide difference between “specific pathogen-free” and “pathogen free.” Specific-pathogen-free (SPF) animals and eggs are defined as ‘free of specified microorganisms and parasites, but not necessarily free of others not specified’ (International Committee on Laboratory Animals, 1964). The distinction is important because unidentified pathogens could be present in eggs, avian cultures and chicken fibroblasts that could be part of the final egg-based vaccine. We know chicken stuff is in the vaccines – it’s listed as an ingredient on package inserts.
Could viruses that are harmless to their animal host be dangerous to humans? This grave concern is more than theoretical: Harmful “extra” viruses have been passed on in vaccines before.
Over the last 15 to 20 years researchers have recorded a litany of serious disease-causing transmissions passed on to humans through vaccines. In the 1940s, hepatitis B was transmitted via the albumen used to stabilize the yellow fever vaccine. Between 1955 and 1962, at least a third of all polio vaccines were contaminated with the cancer-causing virus SV40. Debbie Bookchin and Jim Schumacher chronicled the story in their meticulously written book, The Virus and The Vaccine (my book review is here.) Similarly, human blood products, blood-derived materials, and clotting factors have transmitted various infectious agents and viruses over the years. Both hepatitis C and paravirus B-19 have been passed to hemophiliacs through contaminated products.
Avian Leucosis Virus
One virus that has garnered a great deal of attention is avian leucosis virus, or ALV. For more than 50 years, this retrovirus has been identified in the eggs used to manufacture vaccines. AVL is known to infect large segments of the poultry industry. A slowly oncogenic retrovirus, AVL is responsible for a variety of cancers in chickens, including lymphoid leukosis and erythroblastosis.ALV has the ability to integrate randomly throughout the human genome. One group of researchers who studied the actions of retroviruses writes,
“Oncogene transduction is the process by which a gene is captured by a retrovirus…Serial passage of a retrovirus that does not carry an oncogene leads with high frequency, to the emergence of new viruses that have transduced oncogenes…This model also explains the generation of the vast majority of acutely transforming retroviruses isolated in vivo. ”That is professional double-speak for the following: Given the right conditions, ALV can be integrated into a human chromosome, creating a faulty gene that can go rogue and evolve into cancer.
Endogenous Avian Virus
Another contaminant retrovirus found in eggs is endogenous avian virus, or EAV. This virus is present in all breeds of chicken and cannot be eliminated from even the most stringently kept flocks. EAV has an associated enzyme called reverse transcriptase which copies the genetic material of the virus into the DNA of the host. Since 1982, researchers have known of the presence of EAV and reverse transcriptase in influenza and other egg-based vaccines. In 1999, Tsang, et al. detected the presence of reverse transcriptase in the chicken-cell derived measles and mumps vaccines. His team tracked the enzyme’s origin back to the avian cell cultures the viruses had been grown in.http://vaxxter.com/the_disgusting_stuff_in_eggs/
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FDA approves genetically modified chicken — but not as food
Gathering a drug from chicken egg whites
Dec 9, 2015,
This isn't the first time that the FDA has approved a transgenic animal for drug production. Six years ago, the US government approved genetically modified goats that can make a drug in their milk that prevents blood clots. And in 2014, the FDA approved Ruconest — a drug that's collected from rabbit milk.
But yesterday's approval doesn't rely on milk production. To collect the active protein, researchers have to purify it from the whites of the chicken's eggs. Kanuma works by replacing a malfunctioning enzyme in people with lysosomal acid lipase deficiency.
As part of the approval process for the drug, the government officials looked at whether the alterations made to the chicken's genetic material would cause it harm. They also examined whether these changes are stable enough to be passed on to future generations.
https://www.theverge.com/2015/12/9/9879678/gmo-chicken-transgenic-fda-approved-kanuma-drug-eggs
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Brit scientists create genetically modified chickens that can lay eggs from different breeds
18 Feb 2017
The aim is to preserve rare chicken breeds that may be resistant to global infections like bird flu in the future or have highly desirable features such as excellent meat quality
http://www.mirror.co.uk/science/brit-scientists-create-genetically-modified-9842348
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Genetically Modified Birds
IOSR Journal of Pharmacy and Biological Sciences
http://iosrjournals.org/iosr-jpbs/papers/Vol9-issue6/Version-3/D09631629.pdf
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Researchers Engineer Hens that Make Drugs in Eggs
January 19, 2007
Scientists at the Roslin Institute, which produced Dolly the cloned sheep, have genetically engineered chickens to produce drugs in their egg whites. Helen Sang, the lead scientist at the Roslin Institute in Midlothian, Scotland, talks about the findings.
For instance, they have tweaked cows to produce drugs in their milk so every time the cows give milk, they also give a fresh supply of drugs. But in a twist on this approach, scientists at the Roslin Institute - that's the institute that created Dolly, the cloned sheep - well scientists at Roslin have genetically engineered chickens now to produce drugs in their egg whites. Some of them - some of these drugs that are used to fight cancer.
With every batch of eggs comes a fresh supply of drugs. Unfortunately, getting the drugs isn't as easy as scrambling up an omelet. The researchers report on their research in the current issue of the journal Proceedings of the National Academy of Sciences, and the leader of the team joins us now to talk about their work and its potential to change the way drugs are made.
http://www.npr.org/templates/story/story.php?storyId=6921969
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Targeted mutagenesis in chicken using CRISPR/Cas9 system
23 October 2015
The CRISPR/Cas9 system is a simple and powerful tool for genome editing in various organisms including livestock animals. However, the system has not been applied to poultry because of the difficulty in accessing their zygotes. Here we report the implementation of CRISPR/Cas9-mediated gene targeting in chickens. Two egg white genes, ovalbumin and ovomucoid, were efficiently (>90%) mutagenized in cultured chicken primordial germ cells (PGCs) by transfection of circular plasmids encoding Cas9, a single guide RNA, and a gene encoding drug resistance, followed by transient antibiotic selection. We transplanted CRISPR-induced mutant-ovomucoid PGCs into recipient chicken embryos and established three germline chimeric roosters (G0). All of the roosters had donor-derived mutant-ovomucoid spermatozoa, and the two with a high transmission rate of donor-derived gametes produced heterozygous mutant ovomucoid chickens as about half of their donor-derived offspring in the next generation (G1). Furthermore, we generated ovomucoid homozygous mutant offspring (G2) by crossing the G1 mutant chickens. Taken together, these results demonstrate that the CRISPR/Cas9 system is a simple and effective gene-targeting method in chickens.
https://www.nature.com/articles/srep23980
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Genetic modification of chicken germ cells
Oct, 2012
Over the past two decades numerous reports have demonstrated that the genetic modification of poultry genomes has great potential for improving poultry production; moreover, it may be used as a powerful tool for the production of industrial proteins. To date, transgenic techniques have been established for generating transgenic birds that express recombinant human proteins in hen eggs, as well as tissue-specific genes as an animal model. The production of transgenic birds is a promising approach that could have practical applications in agriculture and biopharmacology, in addition to advancing our understanding of avian biology. Finally, germ cell–mediated transgenesis could provide a more efficient strategy for creating gene-targeted insertions and deletions in avian species.
Recently, van de Lavoir et al.21 genetically modified chicken PGCs by the electroporation of a nonviral expression vector to produce transgenic offspring through germline transmission. However, the frequency of transgene integration into the genome as well as the rate of gene transfer into germ cells remain insufficient for generating transgenic birds using virus-independent conventional methods. For efficient transgene integration into the genome of chicken PGCs, Leighton et al.22 used phiC31 integrase and specific elements. phiC31 integrase catalyzes site-specific recombination between an attB site and an attP site; thus, the co-transfection of an integrase and attB-containing plasmid could improve genomic insertion into chicken PGCs. They showed increased frequencies of transgene integration into endogenous pseudo attP sites in the chicken PGC genome when phiC31 integrase was co-introduced; however, there is no report of the production of transgenic chickens using phiC31 integrase and an attB element. In addition, the in vitro and in vivo silencing of transgene expression following nonviral transfection has hampered the stable expression of antibiotic genes for selection and specific expression in target tissues.21–23 Leighton et al.22 demonstrated that the usage of phiC31 integrase and an attB element could be an efficient tool for genomic insertion; however, they also reported the transcriptional silencing of a transgene in chicken PGCs even after the co-transfection of phiC31 integrase and attB sequences. We previously showed that the methylation of a transgenic promoter in the transgenic chicken could lead to transgene transcriptional silencing in a tissue-specific manner in vivo, although little is known about the control of gene expression in avian species via DNA methylation.23 To overcome this transcriptional repression, HS4 insulator, which is the first insulator identified in vertebrates, derived from the 5' end of the chicken ß-globin locus, has been used in chicken PGCs.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3499655/
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New Hepatitis B Vaccine Found To Massively Increase Heart Attack Odds
http://vaxxter.com/new-hepatitis-b-vaccine-found-to-increase-heart-attack-odds-7-times-normal/
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Heat-stable rotavirus vaccine shows promising results
A new low-cost rotavirus gastroenteritis vaccine that does not require cold storage showed a vaccine efficacy of 67% in a per-protocol test of 3,508 Nigerien infants, according to a study by Sheila Isanaka of the department of research at Epicentre, Paris, and her associates.
In a double blind, placebo-controlled test of the oral bovine rotavirus pentavalent vaccine (BRV-PV), 31 severe cases of rotavirus were found in the vaccinated group of 1,780, while 87 cases were found in the placebo group of 1,728 (2.14 vs. 6.44 cases per 100 person-years, respectively), according to Ms. Isanaka and her colleagues (N Engl J Med. 2017 Mar 23;376[12]:1121-30).
Researchers gathered infants with severe symptoms and administered three injections of either BRV-PV or the placebo from August 2014 through November 2015. The BRV-PV vaccine, manufactured by Serum Institute of India, contains the rotavirus serotypes G1, G2, G3, G4, and G9.
When infants were tested 28 days after the third injection, results showed BRV-PV prevented 4.30 episodes per 100 person years (95% confidence interval, 2.75-5.85). The vaccine also showed stronger efficacy as severity of symptoms increased, with 69% and 77% for severe and extremely severe cases, respectively.
With regard to adverse events, there was no distinction between the two groups (P greater than .15). According to medical investigators, the most common cause of deaths – 27 and 22 in the vaccine and placebo groups, respectively – was infections and infestations (in 37 infants) and metabolism and nutrition disorders (in 6).
Part of what makes this vaccine unique is its shelf life of 2 years when kept at 37° C, or 6 months at 40° C, a key point for Ms. Isanaka and her colleagues.
“The global supply of the vaccines is constrained, and unreliable transportation and storage systems make delivery of vaccines that require refrigeration difficult,” Ms. Isanaka and her colleagues reported. “The introduction of BRV-PV may help to minimize the burden on already strained immunization programs.” The vaccine is also “for sale at or below the current price of the two WHO prequalified vaccines that are supported by the Gavi alliance,” making it possibly more financially accessible as well.
While the per-protocol test found efficacy at 67%, the intention-to-treat population reported a higher efficacy of 73%. Ms. Isanaka and her colleagues believe this number “may more closely represent the efficacy under real-world conditions,” due to the more flexible vaccination schedule.
This study was limited by a short time frame, which did not allow researchers to gather genotyping data pertaining to the efficacy against changing serotypes. BRV-PV also was not given consistently with the oral polio vaccine.
The study was supported by Médecins sans Frontières Operational Center in Geneva and the Kavli Foundation. Ms. Isanaka’s institution, Epicentre, receives core funding from Médecins sans Frontières. Ms. McNeal reports grant support from Epicentre for the study, and other support from Merck and GlaxoSmithKline outside the submitted work. None of the other researchers had relevant financial disclosures.
http://www.mdedge.com/pediatricnews/article/134737/vaccines/heat-stable-rotavirus-vaccine-shows-promising-results
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A thermostable freeze dried rotavirus vaccine formulation and process to prepare thereof
| Publication date | Jan 21, 2016 |
| Filing date | Jul 16, 2015 |
Patent: WO 2016009381 A2
Abstract
The present invention relates to a
thermostable freeze dried rotavirus vaccine formulation and the process
of preparing the same. More specifically the present invention discloses
multivalent thermostable liquid, powder or cake based rotavirus vaccine
formulation prepared using the freeze drying process, such that the
said vaccine formulation possess improved heat-stability, easy to use
and transport and highly affordable thereby meeting the requirements of
developing and low income country's vaccination program. The said freeze
dried rotavirus vaccine formulation along with reconstitution buffer is
so engineered to be suitable for filling in appropriate packaging
containers/ closures so designed such that they reduce the logistics
requirement for storage.
http://www.google.com/patents/WO2016009381A2?cl=en
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Scientists are preparing to “awaken” a 30,000-year-old Frankenvirus from Siberia
9/10/2015
What you’re about to read may sound like the plot of an upcoming science fiction thriller, but we assure you, it’s all real. Scientists are preparing to bring a 30,000-year-old giant virus back to life, after it was unearthed from the frozen wastelands of Siberia. The bug in question, called Mollivirus sibericum, is the fourth type of prehistoric virus found since 2003 and researchers hope that reanimating the virus will help us learn about what’s in store as the permafrost melts. French researchers are currently investigating whether it could pose a potential harm to humans or animals before they revive the so-called Frankenvirus.
http://inhabitat.com/scientists-preparing-to-awaken-a-second-30000-year-old-frankenvirus-from-siberia/
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Some prehistoric viruses evolved to attack certain species of animals, many of these host animals are now extinct. Scientists wonder how these prehistoric viruses will react, when combined with other current living viruses and host species of animals. Would this also give scientists the right to clone animals that were once extinct, in order to do experiments with these prehistoric viruses and organisms?
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Marek’s disease herpesvirus vaccines integrate into chicken host chromosomes yet lack a virus-host phenotype associated with oncogenic transformation
2016
Abstract
Marek’s
disease (MD) is a lymphotropic and oncogenic disease of chickens that
can lead to death in susceptible and unvaccinated host birds. The
causative pathogen, MD virus (MDV), a highly oncogenic alphaherpesvirus,
integrates into host genome near the telomeres. MD occurrence is
controlled across the globe by biosecurity, selective breeding for
enhanced MD genetic resistance, and widespread vaccination of flocks
using attenuated serotype 1 MDV or other serotypes. Despite over 40
years of usage, the specific mechanism(s) of MD vaccine-related immunity
and anti-tumor effects are not known. Here we investigated the
cytogenetic interactions of commonly used MD vaccine strains of all
three serotypes (HVT, SB-1, and Rispens) with the host to determine if
all were equally capable of host genome integration. We also studied the
dynamic profiles of chromosomal association and integration of the
three vaccine strains, a first for MD vaccine research. Our cytogenetic
data provide evidence that all three MD vaccine strains tested integrate
in the chicken host genome as early as 1 day after vaccination similar
to oncogenic strains. However, a specific, transformation-associated
virus-host phenotype observed for oncogenic viruses is not established.
Our results collectively provide an updated model of MD vaccine-host
genome interaction and an improved understanding of the possible
mechanisms of vaccinal immunity. Physical integration of the oncogenic
MDV genome into host chromosomes along with cessation of viral
replication appears to have joint signification in MDV’s ability to
induce oncogenic transformation. Whereas for MD vaccine serotypes, a
sustained viral replication stage and lack of the chromosome-integrated
only stage were shared traits during early infection.
http://www.sciencedirect.com/science/article/pii/S0264410X16308763
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Evaluation of a genetically modified foot-and-mouth disease virus vaccine candidate generated by reverse genetics
15 September 2011
Abstract
Background
Foot-and-mouth disease (FMD) is the most
economically important and highly contagious disease of cloven-hoofed
animals worldwide. Control of the disease has been mainly based on
large-scale vaccinations with whole-virus inactivated vaccines. In
recent years, a series of outbreaks of type O FMD occurred in China
(including Chinese Taipei, Chinese Hong Kong) posed a tremendous threat
to Chinese animal husbandry. Its causative agent, type O FMDV, has
evolved into three topotypes (East–South Asia (ME-SA), Southeast Asia
(SEA), Cathay (CHY)) in these regions, which represents an important
obstacle to disease control. The available FMD vaccine in China shows
generally good protection against ME-SA and SEA topotype viruses
infection, but affords insufficient protection against some variants of
the CHY topotype. Therefore, the choice of a new vaccine strain is of
fundamental importance.
Results
The present study describes the
generation of a full-length infectious cDNA clone of FMDV vaccine strain
and a genetically modified virus with some amino acid substitutions in
antigenic sites 1, 3, and 4, based on the established infectious clone.
The recombinant viruses had similar growth properties to the wild
O/HN/CHA/93 virus. All swine immunized with inactivated vaccine prepared
from the O/HN/CHA/93 were fully protected from challenge with the
viruses of ME-SA and SEA topotypes and partially protected against
challenge with the virus of CHY topotype at 28 days post-immunization.
In contrast, the swine inoculated with the genetically modified vaccine
were completely protected from the infection of viruses of the three
topotypes.
Conclusions
Some amino acid substitutions in the
FMDV vaccine strain genome did not have an effect on the ability of
viral replication in vitro. The vaccine prepared from genetically
modified FMDV by reverse genetics significantly improved the protective
efficacy to the variant of the CHY topotype, compared with the wild
O/HN/CHA/93 virus. Thus, the full-length cDNA clone of FMDV can be a
useful tool to develop genetically engineered FMDV vaccine candidates to
help control porcinophilic FMD epidemics in China.
https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-57
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Genetically Modified Cattle With Human DNA Might Hold Ebola Cure
On a farm outside Sioux Falls, South Dakota, a herd of cloned, genetically engineered cattle are busy incubating antibodies against the Ebola virus.
Researchers hope the cattle – which certainly don’t look like anything special – will produce gallons of blood plasma that could be used to treat people with the deadly virus, which has infected more than 21,000 people in West Africa and killed 8,500 of them.
“These animals produce very high levels of human antibody,” said Eddie Sullivan, president and CEO of SAb Biotherapeutics, the company that developed the cattle.
http://www.nbcnews.com/storyline/ebola-virus-outbreak/genetically-modified-cattle-human-dna-might-hold-ebola-cure-n287796
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Are these genetically engineered cows the future of medicine?
From lab-made cows to gene-altered goats
Dec 3, 2016
They look like normal black-and-white Holstein cows, a
common sight in Western Iowa. But these cows are special: used not for
their milk or meat, but for their blood. They’re plasma donors, and one
day, the life they save may be your own.
The cows were genetically engineered by biotech company
SAB Biotherapeutics to produce human antibodies, proteins that fight
pathogens. These antibodies could one day treat infectious diseases like
Ebola, influenza, and Zika — and their potential to address global
outbreaks was recognized this summer by the World Health Organization.
SAB’s cattle are just the latest example of lab-made animals engineered to be drug factories. Last year, the US Food and Drug Administration approved a genetically modified chicken that makes a drug in its eggs to treat "lysosomal acid lipase deficiency" — a rare genetic condition that prevents the body from breaking down fatty molecules inside cells. In 2014, the FDA approved a drug collected from the milk of lab-made rabbits to treat hereditary angioedema, a genetic disease that causes body swelling and can be fatal. And in 2009, the FDA approved a genetically altered goat that can make a drug in its milk that prevents fatal blood clots.
https://www.theverge.com/2016/12/3/13819482/genetically-engineered-animals-drugs-sab-cows-pharming-future
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Malaria vaccine in A&M goats' milk could save lives
March 3, 2012
Worldwide toll
Malaria, a mosquito-borne disease, killed about 655,000 people in 2011, according to the World Health Organization. The Institute for Health Metrics and Evaluation at the University of Washington puts the death toll much higher: 1.2 million.
Bioengineered animals could be life-savers for Third World countries that cannot afford to build multimillion-dollar facilities to produce vaccines, according to Westhusin and associate professor Charles Long.
Goats are indigenous in all the major impoverished areas, Westhusin noted.
"They are easy to keep. They can eat a beer can and turn it into protein and milk," he said. "They are just great animals in terms of what they offer to impoverished countries."
The vaccine currently is in a form that must be isolated, purified and injected, researchers said. A&M will send No. 21's milk to GTC Biotherapeutics for continued testing and trials.
The Massachusetts-based firm originally developed the transgenic malaria vaccine, which proved effective in mice, said William Gavin, GTC vice president of farm operations and chief veterinarian.
The word "transgenic" means "transferring or having genes from another species." To create the malaria vaccine, DNA coding for the malaria parasite is introduced into the goat genome linked to milk production. The new DNA switches on in the mammary gland only when the animal produces milk, according to GTC.
http://www.chron.com/news/houston-texas/article/A-amp-M-goats-modified-to-carry-malaria-vaccine-3378385.php
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Chemists Re-Create Deadly Frog Poison In The Lab
November 17, 2016
The golden poison dart frog is about an inch long and banana yellow. By some estimates, the skin of one little frog contains enough toxin to kill 10 adult men.
"Oh yeah, it's one of the more lethal poisons on the planet," says Justin Du Bois, a synthetic chemist at Stanford University.
The substance is called batrachotoxin (buh-TRAK-uh-TOX-in), and tiny amounts of it can be deadly if it makes it into a victim's bloodstream. It's what some indigenous groups in Colombia's lowland rain forest would use to tip their blow darts.
And, as Du Bois and his colleagues write Thursday in the journal Science, they figured out how to make it in the lab in 24 steps. Why on Earth would anyone want to do that?
"Well, it turns out it's a fantastic research tool for figuring out how nerves conduct electricity," Du Bois says, "and we're very interested in that fundamental process."
Once inside a victim, the compound embeds itself in certain proteins that are responsible for conducting electrical impulses through the nerves and muscles, including the heart. By disrupting that process, it can cause paralysis and a heart attack. But studying the poison's mode of action could also lead to a deeper understanding of the role electrical impulses play in fundamental processes like heart function and the sensation of pain.
Olivera's lab found that the venom that certain sea snails — Conus magus — use to paralyze fish also acts on an important mechanism in the human body's communication of pain. That led to the development of a painkiller that is now used in patients who have become tolerant to morphine.
http://www.npr.org/sections/health-shots/2016/11/17/502324984/chemists-recreate-deadly-frog-poison-in-the-lab
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Poison as Medicine
March 24, 2015
The more we learn about how venoms cause their awful damage, the more we realize, medically speaking, how useful they can be.
Bees – and some other species in the order Hymenoptera, such as ants and wasps – are armed with a potent sting that many of us are all too aware of. This is their venom, and it’s a mixture of many compounds. Perhaps the most important is a tiny 26-amino-acid peptide called melittin, which constitutes more than half of the venom of honey bees and is found in a number of other bees and wasps. This little compound is responsible for the burning pain associated with bee stings. It tricks our bodies into thinking that they are quite literally on fire.
When we experience high temperatures, our cells release inflammatory compounds that activate a special kind of channel, TRPV1, in sensory neurons. This ultimately causes the neurons to send a signal to the brain that we’re burning. Melittin subversively makes TRPV1 channels open by activating other enzymes that act just like those inflammatory compounds.
Jellyfish and other creatures also possess TRPV1-activating compounds in their venoms. The endpoint is the same: intense, burning pain.
Despite the wealth of history, the practical application of venoms in modern therapeutics has been minimal. That is, until the past ten years or so, according to Glenn King at the University of Queensland in Brisbane, Australia. In 1997, when Ellie was bouncing around from doctor to doctor, King was teasing apart the components of the venom from the Australian funnel-web, a deadly spider. He’s now at the forefront of venom drug discovery.
King’s group was the first to put funnel-web venom through a separation method called high-performance liquid chromatography (HPLC), which can separate out different components in a mixture based on properties like size or charge. “I was just blown away,” he says. “This is an absolute pharmacological goldmine that nobody’s really looked at. Clearly hundreds and hundreds of different peptides.”
Over the course of the 20th century, suggested venom treatments for a range of diseases have appeared in scientific and medical literature. Venoms have been shown to fight cancer, kill bacteria, and even serve as potent painkillers – though many have only gone as far as animal tests. At the time of writing, just six had been approved by the US Food and Drug Administration for medical use (one other – Baltrodibin, adapted from the venom of the Lancehead snake – is not FDA approved, but is available outside the US for treatment of bleeding during operations).
The more we learn about the venoms that cause such awful damage, the more we realize, medically speaking, how useful they can be. Like the melittin in bee venom.
Melittin does not only cause pain. In the right doses, it punches holes in cells’ protective membranes, causing the cells to explode. At low doses, melittin associates with the membranes, activating lipid-cutting enzymes that mimic the inflammation caused by heat. But at higher concentrations, and under the right conditions, melittin molecules group together into rings creating large pores in membranes, weakening a cell’s protective barrier and causing the entire cell to swell and pop like a balloon.
Because of this, melittin is a potent antimicrobial, fighting off a variety of bacteria and fungi with ease. And scientists are hoping to capitalize on this action to fight diseases like HIV, cancer, arthritis and multiple sclerosis.
For example, researchers at the Washington University
School of Medicine in St Louis, Missouri, have found that melittin can
tear open HIV’s protective cell membrane without harming human cells.
This envelope-busting method also stops the virus from having a chance
to evolve resistance. “We are attacking an inherent physical property of
HIV,” Joshua L Hood, the lead author of the study, said in a press
statement. “Theoretically, there isn’t any way for the virus to adapt to
that. The virus has to have a protective coat.” Initially envisioned as
a prophylactic vaginal gel, the hope is that melittin-loaded
nanoparticles could someday be injected into the bloodstream, clearing
the infection...
Bryan Fry, a colleague of Glenn King’s at the University of Queensland and one of the world’s most prolific venom researchers, says the captopril family and its derivatives still command a market worth billions of dollars a year. Not bad for something developed in 1970s. “It’s not only been one of the top twenty drugs of all time,” he says, “it’s been one of the most persistent outside of maybe aspirin.”
And it’s not just captopril. Fry points to exenatide, a molecule found in the venom of a lizard, the gila monster, and the newest venom-derived pharmaceutical on the US market. Known by the brand name Byetta, this has the potential to treat type 2 diabetes, stimulating the body to release insulin and slow the overproduction of sugar, helping reverse the hormonal changes caused by the disease....
http://discovermagazine.com/2015/april/00-poison-medicine
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Medicinal Uses of Bee Venom
Components of Bee Venom
Scientists do not definitively understand how bee venom, which is a complex mixture of numerous compounds, acts on the human body. However, a number of components of bee venom that have been identified and studied include:
Mellitin
Adolapin
Apamine
A Sting or a Shot: Administering Bee Venom
Before the invention of the syringe, bee venom was always administered directly from bees via the bee's stinger. Today, in some cases, it is still administered in the same way. The live bee is held (with tweezers or some other small instrument) by the person administering the bee venom, who then places the bee on the part of the patient's body to be treated, at which point the bee reflexively stings. Depending on the condition, the treatment schedule can vary. The venom can also be given via a syringe, rather than directly from the bee.Allergic Reactions and Drug Interactions
The greatest risk of bee venom therapy is the risk of a severe allergic reaction, including anaphylactic shock , which can cause a person to stop breathing. If not treated immediately, anaphylactic shock can result in death. Though only a small percentage of the population is allergic to bee venom, it is very important that the person is tested for a bee sting allergy before the treatment. The health care professional who gives the bee therapy should also have a bee sting kit on site in case of an emergency.Considering Bee Venom Therapy
If you are considering bee venom therapy, you must recognize that such therapy is a natural treatment for which, to date, there is no rigorous scientific evidence proving its medicinal effectiveness. Before trying this therapy, talk to your doctor, and remember that this therapy should be used in addition to, not instead of, other treatments prescribed by your doctor. And never have bee venom injections without knowing if you have a bee sting allergy.https://www.allegiancehealth.org/wellness/article/13504
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Bee sting vaccine opens door to new allergy remedies
January, 2017
A BEE vaccine developed in Australia is opening doors to new remedies for a range of other insect, plant and pollen allergies.
Researchers from Adelaide in South Australia have developed a highly effective vaccine for European honeybee stings using a unique adjuvant.
The trials, which were completed last year, were successful but involved only laboratory tests. In response to these results, researchers are now conducting clinical trials on humans using ant venom therapy followed by sting challenges to further test the effectiveness of the adjuvant in insect sting vaccines.
Insect sting allergies affect more than five per cent of the United States population according to the American College of Allergy, Asthma and Immunology and about 50 million people are affected by nasal allergies.
The delta-inulin could potentially also be used as a nasal vaccine against allergies such as pollen or dust but the research has not yet extended into peanut or other food allergies.
http://www.theleadsouthaustralia.com.au/industries/health/bee-sting-vaccine-opens-door-to-new-allergy-remedies/
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How Wasps Use Viruses to Genetically Engineer Caterpillars
And caterpillars might be using the same viral genes to defend themselves against other viruses.
Sep 17, 2015
Sep 17, 2015
https://www.theatlantic.com/science/archive/2015/09/parasitic-wasps-genetically-engineer-caterpillars-domesticated-viruses/405874/
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History of genetic engineering
https://en.wikipedia.org/wiki/History_of_genetic_engineering
---------------------------------------------
Genetic engineering
https://en.wikipedia.org/wiki/Genetic_engineering
------------------------------------------
Vaccines are Epigenetic - If You are Vaccinated You are Genetically Modified
http://www.vaccineriskawareness.com/Vaccines-are-Epigenetic-If-You-are-Vaccinated-You-are-Genetically-Modified
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Leaked Pentagon Video Shows Vaccine Designed to Modify Behavior
http://vaxxter.com/leaked-pentagon-video-shows-vaccine-designed-to-modify-behavior/
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Kinetics of asthma- and allergy-associated immune response gene expression in peripheral blood mononuclear cells from vaccinated infants after in vitro re-stimulation with vaccine antigen.
2008
Abstract
The
global expression of immune response genes in infants after vaccination
and their role in asthma and allergy is not clearly understood.
Pharmacogenomics is ideally suited to study the involved cellular
responses, since the expression of thousands of genes can be assessed
simultaneously. Here, array technology was used to assess the expression
kinetics of immune response genes with association to asthma and
allergy in peripheral blood mononuclear cells (PBMC) of five healthy
infants after vaccination with Infanrix-Polio+Hib. At 12h after in vitro
re-stimulation of the PBMC with pertussis toxin (PT) antigen, 14 immune
response pathways, 33 allergy-related and 66 asthma-related genes were
found activated.
https://www.ncbi.nlm.nih.gov/pubmed/18336961
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The Pseudoscience Of Vaccines – Big Pharma’s Final Solution
August 13, 2020
https://themadtruther.com/2020/08/13/the-pseudoscience-of-vaccines-big-pharmas-final-solution/
----------------------
Shoulder injury related to vaccine administration reported more frequently
September 01, 2017
https://www.aappublications.org/news/2017/09/01/IDSnapshot082917
Shoulder injury related to vaccine administration (SIRVA) is believed to be caused by an immune response following inadvertent, direct injection of a vaccine into the deltoid bursa or joint space.
The presentation of SIRVA typically includes rapid onset of severe, long-lasting shoulder pain following vaccination in the deltoid muscle, resultant limited range of motion and absence of infection. Data from the Vaccine Adverse Event Reporting System suggest SIRVA is being reported with increasing frequency.
Which of the following statements regarding vaccine administration are correct?
a) The suggested route of administration for each vaccine is recommended by the manufacturer and is based on studies showing maximum safety and immunogenicity.
b) The presence or absence of an adjuvant is not a factor when considering vaccine administration.
c) For most infants younger than 12 months of age, the anterolateral thigh muscle is the preferred site because it has more muscle mass than the deltoid muscle.
d) The buttock generally should not be used for active immunization because of limited absorption from gluteal fat.
Answer: a, c and d are correct
Vaccines should be administered in an anatomic area where neural, vascular or tissue injury is unlikely to occur. For intramuscular injections, the needle length should be long enough to ensure injection occurs in the muscle mass. Too long a needle length increases the risk that injection may involve nerves, blood vessels or skeletal structures. Suggested needle lengths are presented in the 2015 Red Book (Table 1.7, page 28, http://bit.ly/2tgo990). Most intramuscular injections are performed with a 22- to 25-gauge needle.
Injectable vaccines are administered by the intramuscular, subcutaneous or intradermal routes except for the smallpox vaccine, which is administered by the percutaneous route using a bifurcated needle (scarification).
Selection of the proper injection site and needle length depends on the amount of muscle and adipose tissue at the selected site, the child’s age and the volume to be injected. Inactivated vaccines containing an adjuvant should be injected into muscle to avoid the risk of local irritation, skin discoloration and granuloma formation that may be associated with subcutaneous injection.
For infants less than 1 month of age, a 5/8-inch needle is suggested for injection in the anterolateral thigh. For term infants 1 through 12 months of age, a 1-inch needle is suggested. For toddlers and children, either the anterolateral thigh or deltoid muscles are suggested. If two vaccines are administered in the same limb at the same visit, they should be spaced 1-inch apart.
Transient, mild shoulder discomfort following immunization in the deltoid muscle is a common side effect of vaccination. Severe, persistent shoulder pain in association with prolonged limitation of function is rare.
SIRVA identifies a specific condition that is associated with vaccine inadvertently administered into the deltoid bursa or joint space. Patients with SIRVA experience shoulder injury that is more severe than would be expected from just needle trauma. One theory suggests that an immune reaction to one or more components of the vaccine may be responsible for signs and symptoms of SIRVA.
In a series of 13 cases among adult patients published by the Vaccine Injury Compensation Program (Atanasoff S, et al. Vaccine. 2010;28:8049-8052), shoulder pain was noted immediately after vaccination in 50% of cases, and pain developed in 90% within 24 hours. The most common findings on physical examination were painful and limited range of motion. Arm weakness and sensory changes were uncommon. Deep tendon reflexes were normal. Symptoms persisted six months to several years, and 30% of patients required surgery.
Several theories have been proposed to explain why SIRVA is reported less frequently in children, despite the number of vaccines administered. Administration in the anterolateral thigh avoids the risk of joint involvement; bunching of the subcutaneous and deltoid tissue prior to vaccination may increase the distance to the shoulder; and the developing subacromial bursa may be less developed (smaller) in children.
Most cases in adults occur after administration of a vaccine to which some immunity already exists because of previous immunization such as influenza or tetanus-containing vaccines. This may result in a greater inflammatory response following inadvertent injection into the skeletal structures of the shoulder.
The number of people for whom compensation for SIRVA was awarded by the Vaccine Injury Compensation Program in 2016 was 202 cases. Many instances of SIRVA may be avoided by proper vaccination technique and positioning.
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“Genetically modified” vaccines and GMOs: Sapping and impurifying all our precious bodily fluids?
October 2, 2012
...Oh, noes! The same techniques used to produce GMOs are also used to make some vaccines! Horrors! Yes, Joe, it’s molecular biology and biotechnology. The techniques behind biotechnology are indeed the same, because that’s all they are: Techniques. These are techniques that have a broad utility to a wide variety of applications. These techniques allow the introduction and alteration of DNA to useful ends, be it to modify bacteria or other cells to make proteins in large quantities, to study cell function, or, yes, to make food or vaccines. Whether the products of these techniques are safe or not is not a function of the broad technique of “genetic modification” (GM) but a function of what is done with it.
http://scienceblogs.com/insolence/2012/10/02/genetically-modified-vaccines-and-gmos-sapping-and-impurifying-all-our-precious-bodily-fluids/
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Genetically modified virus
https://en.wikipedia.org/wiki/Genetically_modified_virus
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Protective Immunity and Safety of a Genetically Modified Influenza Virus Vaccine
2014
Abstract
Recombinant influenza viruses are promising viral platforms to be used as antigen delivery vectors. To this aim, one of the most promising approaches consists of generating recombinant viruses harboring partially truncated neuraminidase (NA) segments. To date, all studies have pointed to safety and usefulness of this viral platform. However, some aspects of the inflammatory and immune responses triggered by those recombinant viruses and their safety to immunocompromised hosts remained to be elucidated. In the present study, we generated a recombinant influenza virus harboring a truncated NA segment (vNA-Δ) and evaluated the innate and inflammatory responses and the safety of this recombinant virus in wild type or knock-out (KO) mice with impaired innate (Myd88 -/-) or acquired (RAG -/-) immune responses. Infection using truncated neuraminidase influenza virus was harmless regarding lung and systemic inflammatory response in wild type mice and was highly attenuated in KO mice. We also demonstrated that vNA-Δ infection does not induce unbalanced cytokine production that strongly contributes to lung damage in infected mice. In addition, the recombinant influenza virus was able to trigger both local and systemic virus-specific humoral and CD8+ T cellular immune responses which protected immunized mice against the challenge with a lethal dose of homologous A/PR8/34 influenza virus. Taken together, our findings suggest and reinforce the safety of using NA deleted influenza viruses as antigen delivery vectors against human or veterinary pathogens.
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0098685
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Herpes virus genetically engineered to destroy skin cancer cells
May 27, 2015
http://newatlas.com/herpes-virus-skin-cancer-treatment/37714/
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Cancer-fighting viruses win approval
October 25, 2015
US regulators clear a viral melanoma therapy, paving the way for a promising field with a chequered past.
An engineered herpesvirus that provokes an immune response against cancer has become the first treatment of its kind to be approved for use in the United States, paving the way for a long-awaited class of therapies. On 27 October, the US Food and Drug Administration (FDA) approved a genetically engineered virus called talimogene laherparepvec (T-VEC) to treat advanced melanoma. Four days earlier, advisers to the European Medicines Agency had endorsed the drug.
US regulators clear a viral melanoma therapy, paving the way for a promising field with a chequered past.
An engineered herpesvirus that provokes an immune response against cancer has become the first treatment of its kind to be approved for use in the United States, paving the way for a long-awaited class of therapies. On 27 October, the US Food and Drug Administration (FDA) approved a genetically engineered virus called talimogene laherparepvec (T-VEC) to treat advanced melanoma. Four days earlier, advisers to the European Medicines Agency had endorsed the drug.
http://www.nature.com/news/cancer-fighting-viruses-win-approval-1.18651
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Genetically Modified Live Attenuated Parasites as Leishmania vaccines :
Evaluation of Safety and Efficacy
2006
https://www.fda.gov/ohrms/dockets/ac/06/slides/2006-4226S1_14.pdf
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A Genetically Engineered Live Attenuated Vaccine of Coccidioides posadasii Protects BALB/c Mice against Coccidioidomycosis
2007
ABSTRACT
Coccidioidomycosis (also known as San
Joaquin Valley fever) is an occupational disease. Workers exposed to
outdoor dust which
contains spores of the soil-inhabiting fungus have a
significantly increased risk of respiratory infection. In addition,
people
with compromised T-cell immunity, the elderly, and
certain racial groups, particularly African-Americans and Filipinos, who
live in regions of endemicity in the southwestern
United States have an elevated incidence of symptomatic infection caused
by inhalation of spores of Coccidioides posadasii or Coccidioides immitis.
Recurring epidemics and escalation of medical costs have helped to
motivate production of a vaccine against valley fever.
The major focus has been the development of a
defined, T-cell-reactive, recombinant protein vaccine. However, none of
the
products described to date have provided full
protection to coccidioidal disease-susceptible BALB/c mice. Here we
describe
the first genetically engineered, live, attenuated
vaccine that protects both BALB/c and C57BL/6 mice against
coccidioidomycosis.
Two chitinase genes (CTS2 and CTS3)
were disrupted to yield the attenuated strain, which was unable to
endosporulate and was no longer infectious. Vaccinated
survivors mounted an immune response characterized
by production of both T-helper-1- and T-helper-2-type cytokines.
Histology
revealed well-formed granulomas and markedly
diminished inflammation. Significantly fewer organisms were observed in
the lungs
of survivors than in those of nonvaccinated mice.
Additional investigations are required to further define the nature of
the
live, attenuated vaccine-induced immunity against Coccidioides infection.
http://iai.asm.org/content/77/8/3196.full
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Here's What '60 Minutes' Didn't Tell You About The 'Miracle' Glioblastoma Treatment
Mar 2015
Last night, newsmagazine 60 Minutes devoted not one but two segments to an early-stage trial at Duke University of a cancer therapy that some patients are calling a “miracle.” It’s a genetically modified form of the polio virus, injected directly into the brains of patients with glioblastoma, a particularly deadly type of brain tumor. Eleven of the 22 patients treated so far died, but the other 11 have seen their tumors shrink. Three featured in the story are cancer-free.
https://www.forbes.com/sites/arleneweintraub/2015/03/30/heres-what-60-minutes-didnt-tell-you-about-the-miracle-glioblastoma-treatment/#894b66741e24
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Zika Virus, Microcephaly, Guillain-Barré, Genetically Modified Mosquitos and Potential Vaccine Damage
Published on February 12, 2016
The Japan Times
says, “Many experts and environmental activists alerted on how some
agricultural chemicals — such as paraquat, which has been classified as
“highly poisonous” by U.S. regulators — which have been banned or
phased out in leading industrialized nations, continue being
extensively used in many developing countries, notable Brazil. They are
particularly used in Brazil’s northeast, where almost a third of the
microcephaly cases have occurred so far. In the last few years, Brazil
has become one of the top exporters of orange juice, sugar, coffee,
beef, poultry and soybeans. Brazil’s huge production of those items is
probably due, in part, to the extensive use of pesticides, many of them
highly toxic to humans.”
A report from the Argentine doctors’ organization, Physicians in the Crop-Sprayed Towns, also challenges the theory that the Zika virus epidemic in Brazil is the cause of the increase in the birth defect microcephaly among newborns. Instead of feeding into the standard virus mania this organization is pointing out that in the area where most sick people live, a chemical larvicide that produces malformations in mosquitoes was introduced into the drinking water supply in 2014. This poison, Pyriproxyfen, is used in a State-controlled program aimed at eradicating disease-carrying mosquitoes.
From the doctors at Red Universitaria de Ambiente y Salud (the Red University of Environment and) we have the statement, “A dramatic increase of congenital malformations, especially microcephaly in newborns, was detected and quickly linked to the Zika virus by the Brazilian Ministry of Health. However, they fail to recognize that in the area where most sick persons live, a chemical larvicide producing malformations in mosquitoes has been applied for 18 months, and that this poison (pyroproxyfen) is applied by the State on drinking water used by the affected population.”
A report from the Argentine doctors’ organization, Physicians in the Crop-Sprayed Towns, also challenges the theory that the Zika virus epidemic in Brazil is the cause of the increase in the birth defect microcephaly among newborns. Instead of feeding into the standard virus mania this organization is pointing out that in the area where most sick people live, a chemical larvicide that produces malformations in mosquitoes was introduced into the drinking water supply in 2014. This poison, Pyriproxyfen, is used in a State-controlled program aimed at eradicating disease-carrying mosquitoes.
From the doctors at Red Universitaria de Ambiente y Salud (the Red University of Environment and) we have the statement, “A dramatic increase of congenital malformations, especially microcephaly in newborns, was detected and quickly linked to the Zika virus by the Brazilian Ministry of Health. However, they fail to recognize that in the area where most sick persons live, a chemical larvicide producing malformations in mosquitoes has been applied for 18 months, and that this poison (pyroproxyfen) is applied by the State on drinking water used by the affected population.”
http://drsircus.com/general/zika-virus-microcephaly-guillain-barre-genetically-modified-mosquitos-and-potential-vaccine-damage/
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What do Jellyfish have to do with Vaccines?
(A Comparison of Red Fluorescent Proteins to Model DNA Vaccine Expression by Whole Animal In Vivo Imaging)
PLOS One, June 2015
Vaccines are an incredibly potent tool in our arsenal to prevent infections. Simplistically they work by exposing the immune system to a safe part of the infectious bacteria or virus and evoking a memory response. When the vaccinated individual encounters the actual bug in real life, this memory allows the immune system to recognise and respond to the bug faster, thus preventing the infection before it takes hold. A critical step in vaccine development is the identification, isolation and mass production of the parts of the virus and bacteria that are able to evoke an immune memory. These components are often made up of proteins, which can be problematic and expensive to make to a standard that is acceptable for use as a medicine. Recently an alternative approach has been developed called DNA vaccination, which we describe in this review. DNA, the material of genes, contains the coding message for the production of proteins by cells. In the 1990’s a number of groups made the breakthrough observation, that if you inject DNA encoding proteins from a pathogen into a mouse, the mouse will make the protein in its cells and generate an immune response to this protein. This technique has the potential to significantly change vaccines because it is cheaper, easier and faster to make, for example, if a new viral epidemic occurs e.g. Ebola or MERS-CoV the time to make and test a DNA vaccine could be significantly shorter and therefore the spread of the disease would be reduced.
However, DNA vaccines have performed poorly in early phase clinical trials (i.e. they work really well in mice, but poorly in people). There have been a number of reasons proposed for this failure, but one possibility is that, after immunisation, the genes encoded by the DNA vaccine are not turned into protein by the patient (technically referred to as poor expression). In our recent paper, we set out to address this problem in our study, using a novel technology called in vivo imaging, which uses the proteins that allow jellyfish to glow in the dark and fireflies to flash at night. The original fluorescent protein was green (named green fluoresecent protein), but since then a whole colour chart of proteins has been developed with names like tdTomato and mPlum. If the proteins are injected into mice, they can, using a special camera, be seen. The colour of the protein has a significant impact on our ability to see it in mice, blue and green proteins work poorly because the skin has evolved to reflect light in these wavelengths as a defence against damage caused by UV light. Red proteins tend to be better and in our recently published paper we tested a range of red proteins delivered as DNA, called tdTomato, mCherry, mKatushka and tdKatushka. We observed that only tdTomato gave us a strong enough signal to see in the mice and that there are number of confounding problems with the approach – pens we use to identify individual mice fluoresce in the same colour as mouse poo.
https://www.linkedin.com/pulse/what-do-jellyfish-have-vaccines-john-tregoning
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A Comparison of Red Fluorescent Proteins to Model DNA Vaccine Expression by Whole Animal In Vivo Imaging
2015
Introduction
The use of DNA encoding antigen as a vaccine has many attractive features [1]. These include ease and speed of antigen development, temperature stability of the vaccine and similar patterns of post-translational modification to intracellular pathogens [2]. The ease of development of DNA vaccines is especially of interest for viral pathogens, for example influenza, that require regular updating to match circulating strains or rapid development to prevent fast spreading pandemics. However, whilst extremely effective in small animal models, DNA vaccines have failed to realise their potential in human clinical trials [3]. One factor limiting the progression of DNA vaccines into the clinic may be poor expression levels in human tissue after immunisation [4,5].One approach to understand and improve DNA vaccine delivery is to track expression in vivo using fluorescent proteins. Since the discovery and development of green fluorescent protein [6], there has been a rainbow explosion of available proteins [7]. Fluorescent proteins have a number of characteristics that affect their efficacy as in vivo markers of expression, including wavelength, brightness and structure. The excitation and emission wavelengths of the protein used affects background fluorescence, blue or green fluorescent proteins tend to have higher levels of background because skin reflects light in the shorter/blue wavelengths of the spectrum, as an adaptation to protect against UV induced DNA damage [8]. To reduce background, fluorescent proteins have been designed in the red and far-red spectra [9–11], which promise better signal to noise ratios. A wider gap between excitation and emission can also reduce background by reducing the acquisition of reflected light from the excitation beam. The brightness (degree of fluorescence of the protein) is a function of a number of factors including the quantum yield—the ratio of the number of photons emitted to the number of photons absorbed (the maximum is 1.0); expression maturation and the extinction coefficient—a measure of how strongly the protein absorbs light at a given wavelength. The structure of the protein, particularly whether it is a monomer, dimer or tandem dimer can affect its behaviour in vivo and also has an effect on the cloning of the proteins as tandem repeats may make conventional PCR approaches challenging.
However, most of the studies used to characterise fluorescent proteins in vivo have either imaged injected cells overexpressing the protein [12] or directly injected protein [10]. In order to be a useful tool for the monitoring of gene expression after DNA delivery, the protein needs to have sufficient signal strength following de novo expression in tissue. The aim of the current study was to identify an effective red fluorescent protein for tracking DNA transgene expression in vivo and to explore how expression affects immunogenicity for vaccines. We assessed four fluorescent proteins (tdTomato, mCherry, Katushka and tdKatushka2) which have a range of excitation and emission characteristics, brightness and structures. We observed limited levels of expression in vivo with all the red proteins used, with tdTomato being the strongest. Different delivery routes led to different expression levels, with a stronger fluorescent signal after intramuscular delivery than subcutaneous or tattooing. The difference in visible expression levels did not appear to correlate with the outcome of infection following vaccination, with protection observed against influenza H1N1 infection when a DNA vaccine encoding haemagglutinin was delivered by either the intramuscular, subcutaneous or tattooing routes. This suggests that in vivo whole animal imaging of fluorescent proteins has limited utility for predicting DNA vaccine efficacy.
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0130375
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Use of green fluorescent protein to tag lactic acid bacterium strains under development as live vaccine vectors.
2000
Abstract
The
lactic acid bacteria (LAB) are safe microorganisms which are mainly used
for the preparation of fermented foods and for probiotic applications.
The potential of LAB as live vehicles for the production and delivery of
therapeutic molecules such as antigens is also being actively
investigated today. However, very little is known about the fate of live
LAB when administered in vivo and about the interaction of these
microorganisms with the nasal or gastrointestinal ecosystem. For future
applications, it is essential to be able to discriminate the
biotherapeutic strain from the endogenous microflora and to unravel the
mechanisms underlying the postulated health-beneficial effect. We
therefore started to investigate both aspects in a mouse model with two
LAB species presently under development as live vaccine vectors, i.e.,
Lactococcus lactis and Lactobacillus plantarum. We have constructed
different expression vectors carrying the gfp (green fluorescent protein
[GFP]) gene from the jellyfish Aequoria victoria, and we found that
this visible marker was best expressed when placed under the control of
the inducible strong nisA promoter from L. lactis. Notably, a threshold
amount of GFP was necessary to obtain a bright fluorescent phenotype. We
further demonstrated that fluorescent L. plantarum NCIMB8826 can be
enumerated and sorted by flow cytometry. Moreover, tagging of this
strain with GFP allowed us to visualize its phagocytosis by macrophages
in vitro and ex vivo and to trace it in the gastrointestinal tract of
mice upon oral administration.
https://www.ncbi.nlm.nih.gov/pubmed/10618252
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A Shift From Cell Cultures to Creatures: In Vivo Imaging of Small Animals in Experimental Regenerative Medicine
2011
Although the use of small animals for in vivo experimentation has been widespread, only recently has there been easy availability of techniques that allow noninvasive in vivo imaging of small animals. Because these techniques allow the same individual subject to be followed longitudinally throughout the duration of an experiment, their use is rapidly changing the way small animals are employed in the laboratory. In this review, we focus on six imaging modalities that are increasingly employed for small animal in vivo imaging: optical imaging (OI), magnetic resonance imaging (MRI), computed tomography (CT), single-photon emission tomography (SPECT), ultrasound (US), and positron-emission tomography (PET). Each modality allows for the noninvasive tracking of cells and cell products in vivo. In addition, multimodality imaging, combining two or more of these techniques, has also been increasingly employed to overcome the limitations of each independent technique. After reviewing these available imaging modalities, we detail their experimental application, exemplified by the emerging field of regenerative medicine, referring to publications whose conclusions would otherwise be difficult to support without the availability of in vivo imaging.
http://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(16)32790-3
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Researchers genetically engineer Salmonella to eat brain tumors
01.11.17
Salmonella has earned its bad reputation. It is responsible for more than a million cases of food poisoning every year, of which nearly 400 people die. But a team of researchers from Duke University have recently engineered the bacteria to not attack the human gastrointestinal tract, but rather the most aggressive form of brain cancer known to man.
https://www.engadget.com/2017/01/11/genetically-engineered-salmonella-eats-brain-tumors/
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Impairment of mitochondrial energy metabolism in different regions of rat brain following chronic exposure to aluminium
2008
http://www.sciencedirect.com/science/article/pii/S0006899308017150
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Brain Eating Vaccines: The Reality Behind The “Conspiracy Theory”
August 4, 2010
Wired Magazine writer Jonah Lehrer has labeled concerns that vaccines which alter brain chemistry and induce states of “focused calm” could be abused by governments to create lobotomized, servile populations as delusional, paranoid, and idiotic conspiracy theories, despite the fact that major mental health professionals are already pushing for lithium to be introduced into water supplies as a means of mass medicating against “mood disorders”.
Lehrer, an Oxford University graduate and a Rhodes Scholar, brazenly calls Alex Jones a liar in his article today after Jones put out a You Tube video in which he warned that new vaccines designed to reduce stress and neutralize people’s anger could lead to a nightmare THX 1138 scenario, in which the population is controlled and subjugated through the use of special drugs to suppress emotion.
Jones also encouraged listeners to Google search the words “brain eating vaccines,” causing the term to rise to the number 1 position on Google Trends for August 3rd.
https://www.infowars.com/brain-eating-vaccines-the-reality-behind-the-%E2%80%9Cconspiracy-theory%E2%80%9D/
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New Vaccines Will Permanently Alter Human DNA
Why is the government so maniacal about injecting vaccines?
May 17, 2016
Consider this article in light of the accelerating push to mandate and enforce vaccination across the planet.
The reference is the New York Times, 3/15/15, “Protection Without a Vaccine.” It describes the frontier of research. Here are key quotes that illustrate the use of synthetic genes to “protect against disease,” while changing the genetic makeup of humans. This is not science fiction:
“By delivering synthetic genes into the muscles of the [experimental] monkeys, the scientists are essentially re-engineering the animals to resist disease.”
“’The sky’s the limit,’ said Michael Farzan, an immunologist at Scripps and lead author of the new study.”
“The first human trial based on this strategy — called immunoprophylaxis by gene transfer, or I.G.T. — is underway, and several new ones are planned.”
“I.G.T. is altogether different from traditional vaccination. It is instead a form of gene therapy. Scientists isolate the genes that produce powerful antibodies against certain diseases and then synthesize artificial versions. The genes are placed into viruses and injected into human tissue, usually muscle.”
Here is the punchline: “The viruses invade human cells with their DNA payloads, and the synthetic gene is incorporated into the recipient’s own DNA. If all goes well, the new genes instruct the cells to begin manufacturing powerful antibodies.”
Read that again: “the synthetic gene is incorporated into the recipient’s own DNA.” Alteration of the human genetic makeup. Permanent alteration.
https://www.infowars.com/new-vaccines-will-permanently-alter-human-dna/
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Chapter 6: Nanotechnology & Biotechnology
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Are Populations Being Primed
For Nano-Microchips Inside Vaccines?
It's almost surreal, like something out of a sci-fi flick, but nano-microchips invisible to the naked eye are a reality that are already being hosted in wide-range of applications. The question is, how long will it take governments and big pharma to immerse nano-microchips inside of vaccines to tag and surveil global populations?
http://preventdisease.com/news/09/100509_injectable_nano_microchips.shtml
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Next generation vaccines: single-dose encapsulated vaccines for improved global immunisation coverage and efficacy.
Abstract
OBJECTIVES:
Vaccination is considered the most successful health intervention; yet incomplete immunisation coverage continues to risk outbreaks of vaccine preventable diseases worldwide. Vaccination coverage improvement through a single-dose prime-boost technology would revolutionise modern vaccinology, impacting on disease prevalence, significantly benefiting health care and lowering economic burden of disease.KEY FINDINGS:
Over the past 30 years, there have been efforts to develop a single-dose delayed release vaccine technology that could replace the repeated prime-boost immunisations required for many current vaccines. Biocompatible polymers have been employed to encapsulate model vaccines for delayed delivery in vivo, using either continuous or pulsed release. Biomaterial considerations, safety aspects, particle characteristics and immunological aspects of this approach are discussed in detail.SUMMARY:
Despite many studies showing the feasibility of vaccine encapsulation for single-dose prime-boost administration, none have been translated into convincing utility in animal models or human trials. Further development of the encapsulation technology, through optimising the particle composition, formulation, antigen loading efficacy and stability, could lead to the application of this important approach in vaccine deployment. If successful, this would provide a solution to better global vaccination coverage through a reduction in the number of immunisations needed to achieve protection against infectious diseases. This review provides an overview of single-dose vaccination in the context of today's vaccine needs and is derived from a body of literature that has not been reviewed for over a decade.
© 2015 Royal Pharmaceutical Society.
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EXCLUSIVE: 'Secret plan to inject us ALL with ID chips through vaccination programme'
Oct 23, 2015
A "SINISTER plot to secretly inject tiny identity microchips into people while they receive inoculations against diseases will start within weeks", a former politician has extraordinarily claimed.
http://www.express.co.uk/news/science/614168/Secret-plan-inject-ALL-ID-chips-vaccination-program-Simon-Parkes-conspiracy-theory
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Connecting the Dots: Exposing RFID
Documents and Plans For Surveilling
Radio Frequency Implantable Device (RFID) use on humans can no longer be claimed as speculation or theory. Their use is now well documented and integrated in several planned strategies for the U.S. Department of Health and Human Services and Health Care Bills. These disturbing initiatives will see microchip implants embedded inside every American, and possibly inside every human within the next few years.
http://preventdisease.com/news/09/111509_RFID_chip_documents_plans_for_US_exposed.shtml
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Even some companies have created implantable forms of birth control, such as the implant Nexplanon. I would never want to let any of these chemicals to be implanted in my daughters. What would stop the elite from using this type of technology, to forcibly sterilize women for a form of forced birth control.
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Nexplanon
NEXPLANON is a hormone releasing birth control implant:
https://www.nexplanon.com/?utm_source=3180201&utm_medium=Display&utm_term=79614206&utm_content=203067139&utm_campaign=10790302&dclid=CKyZ24yU6dYCFRKTaQodQ_EEHw
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Synthesized Nanoparticles Act As Artificial Viruses for Gene Therapy
04/09/2015
Researchers of the Nanobiology Unit from the Universitat Autònoma de Barcelona (UAB) Institute of Biotechnology and Biomedicine, led by Antonio Villaverde, managed to create artificial viruses, protein complexes with the ability of self-assembling and forming nanoparticles which are capable of surrounding DNA fragments, penetrating the cells and reaching the nucleus in a very efficient manner, where they then release the therapeutic DNA fragments. The achievement represents an alternative with no biological risk to the use of viruses in gene therapy.
http://www.cemag.us/news/2015/04/synthesized-nanoparticles-act-artificial-viruses-gene-therapy
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PROTACs: A New Type of Drug That Can Target All Disease-Causing Proteins
June 11, 2015
http://scitechdaily.com/protacs-a-new-type-of-drug-that-can-target-all-disease-causing-proteins/
A newly published study from Yale University details the discovery of a new type of drug, called Proteolysis Targeting Chimeras (PROTACs), which can target all disease-causing proteins.
Current drugs block the actions of only about a quarter of known disease-causing proteins, but Yale University researchers have developed a technology capable of not just inhibiting, but destroying every protein it targets.
The new type of drug, called Proteolysis Targeting Chimeras (PROTACs), also can continue to destroy mutant proteins in mouse tumors, according to a new study published June 10 in the journal Nature Chemical Biology.
“This new drug modality culminates a decade of work in the field by my lab,” said Craig Crews, the Lewis B. Cullman Professor of Molecular, Cellular, and Developmental Biology and senior author of the paper, which was done in collaboration with scientists from GlaxoSmithKline and Arvinas, LLC.
Almost all current drugs are small molecules designed to fit into the folds of disease-causing proteins and inhibit their function. High doses are often needed to ensure that protein function is blocked sufficiently to produce therapeutic results, which in turn can produce harmful side effects.
In contrast, PROTACs engage the cells’ own protein degradation machinery to destroy targeted proteins by tagging them for removal and can do so multiple times, meaning it can work at lower doses, the authors say. This suggests this new type of drug has not only the potential to target proteins that are not currently “pharmaceutically vulnerable” but could do so safely, Crews said.
“This is a game-changer for drug development,” Crews said.
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The future of medicine could be found in this tiny crystal ball
February 4, 2016
A Drexel University materials scientist has discovered a way to grow a crystal ball in a lab. Not the kind that soothsayers use to predict the future, but a microscopic version that could be used to encapsulate medication in a way that would allow it to deliver its curative payload more effectively inside the body.
http://phys.org/news/2016-02-future-medicine-tiny-crystal-ball.html?utm_source=menu&utm_medium=link&utm_campaign=item-menu
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Subsurface discovery sprouts a new branch on the tree of life
June 23, 2015
http://phys.org/news/2015-06-subsurface-discovery-tree-life.html#jCp
Bacteria are enigmatic by nature, minuscule but staggeringly—sometimes alarmingly—ubiquitous. As critical drivers of everything from global biogeochemical cycles to million dollar industries, they are the little cogs that keep our planet going. They even control our brains!
The study of bacteria is hindered because the vast majority of them cannot be cultured or grown in the lab. With the 1977 advent of a molecular technique that enabled culture-independent surveys, microbiologists thought they had a handle on the breadth of microbial diversity on earth.
They were wrong.
Last week a study published in Nature pulled the veil on a branch of the bacterial tree of life that has evaded detection for nearly a century and a half. The study, led by Christopher Brown, who is a PhD candidate in microbial biology at the University of California in Berkeley, used cutting edge genome sequencing and savvy bioinformatics techniques to make this remarkable discovery.
It all started at an abandoned uranium milling site on the banks of the Colorado River in a town called Rifle. This location, contaminated with toxic byproducts of uranium milling, has been a test ground for researchers experimenting on how microbes can be harnessed to bioremediate or clean the environment.
Previously, researchers found that when naturally occurring microbes were supplied with a food source—the simple carbon compound acetate—a fortuitous biochemical reaction ensued. When stimulated to grow, a certain group of bacteria utilized the soluble uranium present in the soil and converted it to an insoluble form. Once insoluble, the uranium would be less apt to flow through groundwater and into the adjacent Colorado River.
Researchers posit microscopic processes like this could be capitalized upon at other contaminated sites to prevent the spread of toxins to drinking water.
Current research at the Rifle site aims to delve into the ecology of the small but mighty members of this microbial community. In this most recent study, Brown and colleagues went about this by pumping groundwater through a series of filters to capture cells—some of the tiniest free living organisms ever documented.
DNA from these microbial communities was extracted en masse, sequenced into millions of short snippets, then put back together like a jigsaw puzzle—a one so big it necessitated a powerful supercomputer to finish. In the end, this process yielded nearly 800 complete or near-complete microbial genomes.
Even considering the strength of modern high throughput sequencing technology, the recovery of 800 genomes is impressive—especially considering the very first bacterial genome was completed just 20 years ago and required a prodigious entourage of researchers.
Especially surprising about these genomes recovered by Brown and colleagues is that when they tried to place them on the Tree of Life amongst other known bacteria, they didn't fit.
In these ultra small organisms, the marker genes typically used in the previously mentioned culture-independent identification experiment were riddled with extra stretches of DNA called insertion sequences.
"We were really surprised to find how diverse these groups are within the bacterial domain, and just how consistently different the organisms within this radiation are from the rest of bacteria," Brown said in a statement.
These bacteria cannot be grown in lab cultures. To further complicate things, it is predicted that between 50-100 percent of bacteria in some of the groups they discovered would be completely missed using the standard molecular techniques.
Currently the tree of life is divided into three kingdoms. Bacteria and Archaea are two branches, each composed solely of unicellular organisms. The third kingdom is Eukarya, which encompasses all multicellular life forms and some unicellular microbes as well.
The finding of this paper "represents a substantial modification of the tree of life," corresponding author Jill Banfield said in a statement. "These new major features on the tree of life mean that it probably won't be the simple three-domain view we have now," Banfield said.
These novel bacteria at the Rifle site provide a tantalizing glimpse into microscopic worlds yet to be discovered in other locales. Like the communities at Rifle, other undiscovered bacterial groups could be an untapped resource for beneficial environmental applications.
"People have seen these bacteria in surveys of many different environments all over the planet" Brown said. The next step is to find them.
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Newfound groups of bacteria are mixing up the tree of life
Jun 15, 2015
http://news.berkeley.edu/2015/06/15/newfound-groups-of-bacteria-are-mixing-up-the-tree-of-life/
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Making Drugs Out of Dirt Is Really Hard
January 26, 2015
http://motherboard.vice.com/read/making-drugs-from-dirt-is-really-hard
If you’re looking for new medicines, a good place to search is in the dirt. Many of the molecules that have made human life simpler—like those used i?n antibiotics—have been found by simply analyzing the ground, where microorganisms work relentlessly to synthesize precious, possibly curative stuff.
The good news is that there are very likely still some useful products buried in the world’s dirt. The less good news is that coming across them is very hard.
A team of biologists of Rockefeller University in New York recently launched the website Drug?s from Dirt. The site is a clarion call for people around the world to grab a shovel and send samples of soil to the folks at the university, who will scour them for interesting elements. The same team also published a pa?per this month in which they underline how recent analyses “suggest the existence of an enormous untapped reservoir of natural-product-encoding biosynthetic gene clusters in the environment.”
I got in touch with Sea?n Brady, one of the authors of the paper and head of the university’s Laboratory of Genetically Encoded Small Molecules, to ask him what the deal was with dirt and drugs.
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Origin of Life - How Life Started on Earth
https://www.youtube.com/watch?v=xyhZcEY5PCQ(This is just one theory out of many, the theory is that the atmosphere, water and lightning created new types of building blocks for life on the planet. The theory is ancient bacteria ends up trapped in mud, and scientists are able to find ancient building blocks of life trapped in the mud. The documentary also states that oxygen helped create new chemicals on the planet).
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Assessing the biosimilarity of protein drugs: New study shows method's precision
February 5, 2016
A first-ever interlaboratory study of four versions of a therapeutic protein drug—all manufactured from living cells—reports that an established analytical tool akin to magnetic resonance imaging reliably assessed the atomic structures of the biologically similar products, yielding the equivalent of a fingerprint for each.
The findings, described today in Nature Biotechnology, demonstrate that the method—known as two-dimensional nuclear magnetic resonance spectroscopy, or 2D-NMR—"can be a robust and powerful complementary technique for companies and regulators" when assessing these biosimilars, said Robert Brinson, a research chemist at the National Institute of Standards and Technology (NIST). This type of assessment is part of a set of comparisons required to determine whether a follow-on biological product is highly similar to an existing product, so that there is no "clinically meaningful" difference between the two.
"Other analytical methods provide useful information, but 2D-NMR is one of the few approaches that can yield complete assignment of three-dimensional structure across the entire molecule in solution at atomic-level resolution," Brinson explains. "Our study indicates that 2D-NMR data can yield a precise and unique 'fingerprint' of structural information in a biological product," he said.
http://phys.org/news/2016-02-biosimilarity-protein-drugs-method-precision.html?utm_source=menu&utm_medium=link&utm_campaign=item-menu
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Researchers may have discovered fountain of youth by reversing aging in human cells
May 27, 2015
http://www.gizmag.com/reversal-of-aging-human-cell-lines/37721/
Researchers in Japan have found that human aging may be able to be delayed or even reversed, at least at the most basic level of human cell lines. In the process, the scientists from the University of Tsukuba also found that regulation of two genes is related to how we age.
The new findings challenge one of the current popular theories of aging, that lays the blame for humans' inevitable downhill slide with mutations that accumulate in our mitochondrial DNA over time. Mitochondrion are sometimes likened to a cellular "furnace" that produces energy through cellular respiration. Damage to the mitochondrial DNA results in changes or mutations in the DNA sequence that build up and are associated with familiar signs of aging like hair loss, osteoporosis and, of course, reduced lifespan.
So goes the theory, at least. But the Tsukuba researchers suggest that something else may be going on within our cells. Their research indicates that the issue may not be that mitochondrial DNA become damaged, but rather that genes get turned "off" or "on" over time. Most intriguing, the team led by Professor Jun-Ichi Hayashi was able to flip the switches on a few genes back to their youthful position, effectively reversing the aging process.
The researchers came to this conclusion by comparing the function level of the mitochondria in fibroblast cell lines from children under 12 years of age to those of elderly people between 80 and 97. As expected, the older cells had reduced cellular respiration, but the older cells did not show more DNA damage than those from children. This discovery led the team to propose that the reduced cellular function is tied to epigenetic regulation, changes that alter the physical structure of DNA without affecting the DNA sequence itself, causing genes to be turned on or off. Unlike mutations that damage that sequence, as in the other, aforementioned theory of aging, epigenetic changes could possibly be reversed by genetically reprogramming cells to an embryonic stem cell-like state, effectively turning back the clock on aging.
For a broad comparison, imagine that a power surge hits your home's electrical system. If not properly wired, irreversible damage or even fire may result. However, imagine another home in which the same surge trips a switch in this home's circuit breaker box. Simply flipping that breaker back to the "on" position should make it operate as good as new. In essence, the Tsukuba team is proposing that our DNA may not become fried with age as previously thought, but rather simply requires someone to access its genetic breaker box to reverse aging.
To test the theory, the researchers found two genes associated with mitochondrial function and essentially experimented with turning them on or off. In doing so, they were able to create defects or restore cellular respiration. These two genes regulate glycine, an amino acid, production in mitochondria, and in one of the more promising findings, a 97-year-old cell line saw its cellular respiration restored after the addition of glycine for 10 days.
The researchers' findings were published this month in the journal Scientific Reports.
Whether or not this process could be a potential fountain of youth for humans and not just human fibroblast cell lines still remains to be seen, with much more testing required. However, if the theory holds, glycine supplements could one day become a powerful tool for life extension.
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A barrier against brain stem cell aging
September 17, 2015
Neural stem cells generate new neurons throughout life in the mammalian brain. However, with advancing age the potential for regeneration in the brain dramatically declines. Scientists of the University of Zurich now identified a novel mechanism of how neural stem cells stay relatively free of aging-induced damage. A diffusion barrier regulates the sorting of damaged proteins during cell division.
http://phys.org/news/2015-09-barrier-brain-stem-cell-aging.html#jCp
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Researchers discover surprisingly wide variation across species in genetic systems that influence aging
May 28th, 2015
http://phys.org/news/2015-05-surprisingly-wide-variation-species-genetic.html
A new Iowa State University study focusing on insulin signaling uncovered surprising genetic diversity across reptiles, birds and mammals.
The research sets the stage for an improved understanding of metabolism, growth and aging and may have implications for medicine and human health, said Anne Bronikowski, an associate professor of ecology, evolution and organismal biology and a lead author of the study.
Insulin signaling is a critical biological process that governs the rate at which cells grow and divide and ultimately regulates aging. Scientists previously assumed the process remained much the same throughout the animal kingdom. But the new research shows that the genetic pathways in reptiles evolved to include protein forms not observed in mammals, a finding that suggestions these proteins carry out new or additional functions in reptiles.
The researchers looked at a molecular network known as the insulin/insulin-like signaling and target of rapamycin network (IIS/TOR). Because the IIS/TOR network regulates critical aspects of animal biology, scientists have long speculated that the network would work more or less the same in most animal species.
Bronikowski and Fred Janzen, a professor of ecology, evolution and organismal biology, completed the study along with former and current members of their labs. The research team compared the genomes of mammals and birds with 17 reptile species. The team found an abundance of variation in the hormones and receptors of the network, which bucks the conventional wisdom and indicates that hormones delivered through insulin likely undertake additional functions in reptiles.
"The study provides a critical step toward understanding how the IIS/TOR network may regulate variation in metabolism, modes of reproduction and rates of aging," Bronikowski said. "It highlights genetic variants that occur in nature that may be useful in a human health context. It therefore lays the groundwork for future research to identify natural genetic variants that may work together to alter the function of this network, which may lend insight into metabolic and aging diseases and treatments."
Previous studies of insulin signaling have focused on species commonly used as laboratory models, such as mice, fruit flies and nematodes. The new study compared 66 species, including 17 reptile species for which the research group had to generate transcriptome data – or the set of all RNA molecules in a particular genome – because the data didn't exist previously. The team was able to sequence the reptile data with the help of support from the ISU Center for Integrated Animal Genomics.
The wide range of variation discovered by the study may suggest that the insulin signaling network could be targeted by new medical therapies to treat conditions associated with aging, Bronikowski said.
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"Avatar" project aims for human immortality by 2045
July 25, 2012
http://www.gizmag.com/avatar-project-2045/23454/
Russian media magnate Dmitry Itskov is heading "Avatar," a tremendously ambitious and far-reaching multidisciplinary research project that aims to achieve immortality in humans within the next three decades. He plans to do it by housing human brains in progressively more disembodied vehicles, first transplanting them into robots and then, by the year 2045, by reverse-engineering the human brain and effectively "downloading" human consciousness onto a computer chip.
Fact or fiction?
When speculating on seemingly unobtainable goals such as this, one must be careful not to believe that improbable technological advances automatically become more likely simply by looking further away in the future. This is the cognitive trap that, for instance, has seen many leading IT experts predict the development of a human-level artificial intelligence at roughly twenty years in the future for at least the past five decades
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Can We Reprogram Our DNA and Heal Ourselves With Frequency, Vibration & Energy?
March 5, 2014
http://www.wakingtimes.com/2014/03/05/reprogram-dna-heal-ourselves-frequency-vibration-energy/
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Novel UV-mediated mode of DNA repair
December 22, 2015
UV light damages DNA. But LMU researchers now show that it can also mediate non-enzymatic repair of one type of damage, albeit in a specific context. This effect may have played vital role in early evolution of living systems.The ultraviolet fraction of sunlight triggers photochemical reactions in the DNA molecules that make up the hereditary material in our cells. These reactions can result in alterations in the DNA structure which lead to mutations that may cause cell death or promote tumorigenesis. LMU researchers led by Professor Thomas Carell, who holds the Chair of Bioorganic Chemistry, and Wolfgang Zinth, Professor of Biomolecular Optics, have now shown that the DNA itself can repair one of the most common types of UV-mediated damage by a non-enzymatic mechanism which is itself dependent on UV. The new findings appear in the Journal of the American Chemical Society.
Cells possess a variety of complex, enzyme-based mechanisms which are used for the repair of damaged DNA, and this year's Nobel Prize in Chemistry was awarded to three researchers for work on the elucidation of these mechanisms. The team led by Zinth and Carell, have now discovered the first instance of a sequence-dependent repair mechanism which does not require the participation of enzymes at all.
http://phys.org/news/2015-12-uv-mediated-mode-dna.html
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Scientists Prove DNA Can Be Reprogrammed by Words and Frequencies
Aug 2, 2012
THE HUMAN DNA IS A BIOLOGICAL INTERNET and superior in many aspects to the artificial one. Russian scientific research directly or indirectly explains phenomena such as clairvoyance, intuition, spontaneous and remote acts of healing, self healing, affirmation techniques, unusual light/auras around people (namely spiritual masters), mind’s influence on weather patterns and much more. In addition, there is evidence for a whole new type of medicine in which DNA can be influenced and reprogrammed by words and frequencies WITHOUT cutting out and replacing single genes.
http://wakeup-world.com/2011/07/12/scientist-prove-dna-can-be-reprogrammed-by-words-frequencies/
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Scientists Can Now Pull the DNA of Ancient Humans Out of Cave Dirt
Apr 27, 2017
The technique will allow researchers to study Neanderthals and other prehistoric people without relying on fossils.
https://www.theatlantic.com/science/archive/2017/04/ancient-dna-sediment-neanderthal-denisovan/524433/
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Engineers invent process to accelerate protein evolution
December 7, 2015
All living things require proteins, members of a vast family of molecules that nature "makes to order" according to the blueprints in DNA.
Through the natural process of evolution, DNA mutations generate new or more effective proteins. Humans have found so many alternative uses for these molecules - as foods, industrial enzymes, anti-cancer drugs - that scientists are eager to better understand how to engineer protein variants designed for specific uses.
Now Stanford engineers have invented a technique to dramatically accelerate protein evolution for this purpose. This technology, described in Nature Chemical Biology, allows researchers to test millions of variants of a given protein, choose the best for some task and determine the DNA sequence that creates this variant.
"Evolution, the survival of the fittest, takes place over a span of thousands of years, but we can now direct proteins to evolve in hours or days," said Jennifer Cochran, a professor of bioengineering who co-authored the paper with Thomas Baer, director of the Stanford Photonics Research Center.
http://phys.org/news/2015-12-protein-evolution.html
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Chapter 7: Cancer & Tumor Vaccines
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A Genetically Modified attenuated Listeria Vaccine Expressing HPV16 E7 Kill Tumor Cells in Direct and Antigen-Specific Manner
29 June 2017
http://journal.frontiersin.org/article/10.3389/fcimb.2017.00279/full
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Specific microtubule-depolymerizing agents augment efficacy of dendritic cell-based cancer vaccines
2011
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141632/
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Immunotherapy augments the effect of 5-azacytidine on HPV16-associated tumours with different MHC class I-expression status
2011
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3242529/
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Augmentation of local antitumor immunity in the liver by tumor vaccine modified to secrete murine interleukin 12
1999
Abstract
Minimal residual lesions have been a major problem in surgical management of cancer. We transfected M5076 with murine IL-12 gene by a retroviral vector, established a stable transfectant secreting IL-12 and investigated its antitumor effects on a spontaneous liver metastasis murine model of M5076 reticulum cell sarcoma. Subcutaneous vaccination of the irradiated transfectant into the remote skin following the amputation of the tumor-bearing limb improved survival when compared with the vaccination of irradiated parental cells (control). Cytotoxic activities against parental M5076 were significantly stronger in the hepatic lymphocytes from the mice vaccinated with the IL-12 transfectant than those from the control. IFN-bold gamma production of hepatic lymphocytes when they were cocultured with the parental cells was significantly augmented in mice vaccinated with the IL-12 transfectant compared with the control. On the other hand, both cytotoxic activity and IFN-bold gamma production of spleen cells in the M5076-vaccinated and transfectant-vaccinated mice were at similar levels. Immunophenotypic analysis revealed the selective increase of CD3+NK1+ population in the liver from the transfectant-vaccinated mice. These results suggest that tumor vaccines genetically modified to secrete IL-12 continuously at a relatively low level preferentially augment local antitumor activity in the liver rather than systemic immune responses. This strategy warrants further investigation as an adjuvant modality in the management of postoperative residual tumors.
http://www.nature.com/gt/journal/v6/n6/full/3300916a.html?foxtrotcallback=true
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Significant Antitumor Effects Obtained by Autologous Tumor Cell Vaccine Engineered to Secrete Interleukin (IL)-12 and IL-18 by Means of the EBV/Lipoplex
Mat 2002
http://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(02)90587-3
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ESAT-6-gpi DNA Vaccine Augmented the Specific Antitumour Efficacy Induced by the Tumour Vaccine
B16F10-ESAT-6-gpi/IL-21 in a Mouse Model
2013
http://onlinelibrary.wiley.com/doi/10.1111/sji.12074/pdf
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Tumor-specific CD8+ T cells expressing IL-12 eradicate established cancers in
lymphodepleted hosts
2010
http://cancerres.aacrjournals.org/content/canres/early/2010/07/19/0008-5472.CAN-10-0735.full.pdf
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Development of Cell-Based Tuberculosis Vaccines: Genetically Modified Dendritic Cell Vaccine Is a Much More Potent Activator of CD4 and CD8 T Cells Than Peptide- or Protein-Loaded Counterparts
2005
Abstract
Genetically modified dendritic cell (DC)-based vaccines have not been explored for immunization against tuberculosis. A gene-modified DC vaccine expressing Mycobacterium tuberculosis (M.tb) antigen 85A (Ag85A) was developed by using a recombinant replication-deficient adenoviral gene transfer vector (AdAg85A). AdAg85A-transduced DC vaccine (AdAg85/DC) expressed higher levels of IL-12 and was much more immunogenic than Ag85 protein-loaded (pro/DC) or CD4/CD8 T cell peptide-loaded (pep/DC) DC vaccines. Compared to pro/DC or pep/DC, AdAg85/DC elicited a remarkably higher level of ex vivo IFN-γ production by CD4 and CD8 T cells at weeks 2, 6, and 12 postimmunization, which was coupled with higher frequencies of antigen-specific T cells. By an in vivo CD8 or CD4 T cell cytotoxicity (CTL) assay, AdAg85/DC was shown to provoke much higher and more sustained levels of CD8 and CD4 CTL activity up to 12 weeks postimmunization. Intramuscular (im) AdAg85/DC immunization was more potent than the iv route of AdAg85/DC immunization. Such stronger immunogenicity of im AdAg85/DC vaccination was corroborated with better protection from M.tb challenge. Our results thus suggest that genetically modified DC-based TB vaccine is superior to subunit DC vaccines and has the potential for therapeutic applications.
http://www.cell.com/molecular-therapy-family/molecular-therapy/abstract/S1525-0016(05)01700-4
-----------------------------------------------
Targeting immune response with therapeutic vaccines in premalignant lesions and cervical cancer: hope or reality from clinical studies
14 Jan 2016
http://www.tandfonline.com/doi/full/10.1080/14760584.2016.1176533
-------------------------------------
Development of Membrane-Bound GM-CSF and IL-18 as an Effective Tumor Vaccine
July 17, 2015
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133470
---------------------------------------
Engineered herpes simplex virus expressing IL-12 in
the treatment of experimental murine brain tumors
1999
http://www.pnas.org/content/97/5/2208.full.pdf
------------------------------------------
Effect of HSV-IL12 Loaded Tumor Cell-Based Vaccination in a Mouse Model of High-Grade Neuroblastoma
2016
Abstract
We designed multimodal tumor vaccine that consists of irradiated tumor cells infected with the oncolytic IL-12-expressing HSV-1 virus, M002. This vaccine was tested against the syngeneic neuroblastoma mouse model Neuro 2a injected into the right caudate nucleus of the immunocompetent A/J mice. Mice were vaccinated via intramuscular injection of multimodal vaccine or uninfected irradiated tumor cells at seven and 14 days after tumor establishment. While there was no survival difference between groups vaccinated with cell-based vaccine applied following tumor injection, a premunition prime/boost vaccination strategy produced a significant survival advantage in both groups and sustained immune response to an intracranial rechallenge of the same tumor. The syngeneic but unrelated H6 hepatocellular tumor cell line grew unrestricted in vaccinated mice, indicative of vaccine-mediated specific immunity to Neuro 2a tumors. Longitudinal analyses of tumor-infiltrating lymphocytes revealed a primary adaptive T cell response involving both CD4+ and CD8+ T cell subsets. Spleen cell mononuclear preparations from vaccinated mice were significantly more cytotoxic to Neuro 2a tumor cells than spleen cells from control mice as demonstrated in a four-hour in vitro cytotoxicity assay. These results strongly suggest that an irradiated whole cell tumor vaccine incorporating IL-12-expressing M002 HSV can produce a durable, specific immunization in a murine model of intracranial tumor.
https://www.hindawi.com/journals/jir/2016/2568125/
--------------------------------------------------
Dendritic Cells Genetically Engineered to Simultaneously Express
Endogenous Tumor Antigen and Granulocyte Macrophage
Colony-stimulating Factor Elicit Potent Therapeutic
Antitumor Immunity
https://pdfs.semanticscholar.org/d5d2/c6d0ec01552473526af8d980e00738eb19dd.pdf
------------------------------------------------
Clinical Applications of Dendritic Cell Cancer Vaccines
1999
http://theoncologist.alphamedpress.org/content/4/2/140.full
-----------------------------------------------
Regulation of IL-10 and IL-12 production and function in macrophages and dendritic cells
2015
https://f1000research.com/articles/4-1465/v1
--------------------------------------------
Tumor Cells Engineered with IL-12 and IL-15 Genes Induce Protective Antibody
Responses in Nude Mice
2003
http://www.jimmunol.org/content/jimmunol/171/2/569.full.pdf
------------------------------------------------
The cancer-immunity cycle as rational design for synthetic cancer drugs: Novel DC vaccines and CAR T-cells
Aug 2017
http://www.sciencedirect.com/science/article/pii/S1044579X17300305
--------------------------------------------------
Intranasal Vaccination with Pneumococcal Surface Protein A and Interleukin-12 Augments Antibody-Mediated Opsonization and Protective Immunity against Streptococcus pneumoniae Infection
2001
http://iai.asm.org/content/69/11/6718.full
-----------------------------------------------------
Breast cancer vaccines: maximizing cancer treatment by tapping into host immunity
2005
Abstract
Optimizing standard treatment
modalities for breast cancer has improved the outlook for women
afflicted with it, but the fact
that 40% still ultimately die from the disease
highlights the need for new therapies. Remarkable advances in molecular
immunology
and biotechnology have created a unique
opportunity for developing active vaccination strategies that engage the
patient’s
own immune system in the fight against breast
cancer. Early clinical trials have established the safety and
bioactivity of
some breast cancer vaccine approaches, with a
hint of clinical response. They have also highlighted the importance of
elucidating
the pharmacodynamic interactions between
established therapies for breast cancer, such as tamoxifen, aromatase
inhibitors,
chemotherapy, the HER-2/neu-specific
monoclonal antibody trastuzumab (Herceptin), and breast cancer vaccines.
Preclinical studies have simultaneously
defined the importance of developing targeted
approaches for circumventing established immune tolerance to breast
cancer during
the vaccination process. The first strategies
targeting the negative influence of CD4+CD25+T
regulatory cells and the CTLA-4 signaling pathway are just entering
clinical testing in combination with tumor vaccines.
Developing the most potent approach for
activating antitumor immunity while maintaining the efficacy of standard
approaches
to breast cancer management will ensure that
active immunotherapy is successfully integrated into the standard of
care.
------------------------------------------
Progress on new vaccine strategies for the immunotherapy and prevention of cancer
June 1, 2004
In recent years, great strides in understanding and regulating the immune system have led to new hope for harnessing its exquisite specificity to destroy cancer cells without affecting normal tissues. This review examines the fundamental immunologic advances and the novel vaccine strategies arising from these advances, as well as the early clinical trials studying new approaches to treat or prevent cancer.
https://www.jci.org/articles/view/21926
----------------------------------------------
Cytokine gene-modified vaccines in the therapy of cancer.
1996
Abstract
Therapeutic strategies based on the insertion of cytokine genes into the
genome of tumour cells, followed by vaccination with the resulting
genetically modified, cytokine-producing cells, represent a new
potential prospect for treatment of cancer patients. In this review, the
concept of cytokine gene-modified cancer vaccines is discussed; the
discussion is focused on the rationale, characterization, progress in
the development, preclinical testing, and first clinical trials. An
effort is made to analyse and integrate the results obtained in
different experimental model systems in order to determine the needed
approaches and directions for further research.
http://europepmc.org/abstract/med/8857300
---------------------------------------------------
Whole Tumor Cell Vaccine Adjuvants:
Comparing IL-12 to IL-2 and IL-15
2016
http://iji.sums.ac.ir/article_33404_23d30245ee6d15da7720424bf5957ee2.pdf
----------------------------------------------------
RNA VACCINES
(see also vaccines, therapeutic vaccines, adjuvants and DNA vaccines)
In the context of developing a safe genetic vaccination strategy we tested and studied globin-stabilized mRNA-based vaccination in mice. This vaccination strategy has the advantages of genetic vaccination (easy production, adaptability to any disease and inexpensive storage when lyophilized), but not the drawbacks of DNA vaccination (long-term uncontrolled expression of a transgene, possibility of integration into the host genome and possible induction of anti-DNA antibodies). Injection of naked b-globin untranslated region (UTR)-stabilized mRNA coding for b-galactosidase is followed by detectable translation in vivo. In addition, such a vaccination strategy primes a Th2 type of response which can be enhanced and shifted to a Th1-type immune response by application of recombinant GM-CSF 1 day after mRNA injection. The administration of globin UTR-stabilized mRNA is a versatile vaccination strategy that can be manipulated to fit the requirement of antiviral, antibacterial or antitumor immunityref.
Recently, self-replicating RNA vaccines (RNA replicons) have emerged as an effective strategy for nucleic acid vaccine development. RNA replicon vaccines may be derived from alphavirus vectors, such as Sindbis virusref, Semliki Forest virusref1, ref2, ref3, or Venezuelan equine encephalitis virusref vectors. These vaccines are self-replicating and self-limiting and may be administered as either RNA or DNA, which is then transcribed into RNA replicons in transfected cells or in vivoref1, ref2. Self-replicating RNA is capable of replicating in a diverse range of cell types and allows the expression of the Ag of interest at high levelsref. Self-replicating RNA eventually causes lysis of transfected cellsref1, ref2. Compared with traditional DNA vaccine strategies in which vectors are persistent and the expression constitutive, the expression mediated by the alphaviral vector was transient and lytic. As a result, biosafety risks associated with naked DNA vaccines can be circumvented such as :
- integration into the host genome
- the induction of immunological tolerance
http://www.ufrgs.br/imunovet/molecular_immunology/RNAvaccines.html
--------------------------------------------------
DNA vaccination
https://en.wikipedia.org/wiki/DNA_vaccination
-------------
-------------
-------------
Chapter 8: Lasers & Biophotonics
--------------
--------------
--------------
Laser vaccine adjuvants. History, progress, and potential.
2014
https://www.ncbi.nlm.nih.gov/pubmed/25424797
--------------------------------------------
Laser-powered ‘Needle’ Promises Pain-free Injections
Sept. 13, 2012
WASHINGTON, Sept. 13, 2012—From annual flu shots to childhood immunizations, needle injections are among the least popular staples of medical care. Though various techniques have been developed in hopes of taking the “ouch” out of injections, hypodermic needles are still the first choice for ease-of-use, precision, and control.
A new laser-based system, however, that blasts microscopic jets of drugs into the skin could soon make getting a shot as painless as being hit with a puff of air.
http://www.osa.org/en-us/about_osa/newsroom/news_releases/2012/laser-powered_%E2%80%98needle%E2%80%99_promises_pain-free_injectio/
-----------------------------------------------
Laser Light Could Make Flu Vaccine 7 Times More Effective
Lasers to the rescue, again
http://www.popsci.com/article/science/laser-light-could-make-flu-vaccine-7-times-more-effective
-----------------------------------------------------------
Biophotons: The Human Body Emits, Communicates with, and is Made from Light
Increasingly science agrees with the poetry of direct human experience: we are more than the atoms and molecules that make up our bodies, but beings of light as well. Biophotons are emitted by the human body, can be released through mental intention, and may modulate fundamental processes within cell-to-cell communication and DNA.
http://energyfanatics.com/2017/02/23/biophotons-human-body-emits-communicates-made-light/
-------------------------------------------------
Biophotonics
The term biophotonics denotes a combination of biology and photonics, with photonics being the science and technology of generation, manipulation, and detection of photons, quantum units of light. Photonics is related to electronics and photons. Photons play a central role in information technologies such as fiber optics the way electrons do in electronics.
Biophotonics can also be described as the "development and application of optical techniques, particularly imaging, to the study of biological molecules, cells and tissue". One of the main benefits of using optical techniques which make up biophotonics is that they preserve the integrity of the biological cells being examined.[1][2]
Biophotonics has therefore become the established general term for all techniques that deal with the interaction between biological items and photons. This refers to emission, detection, absorption, reflection, modification, and creation of radiation from biomolecular, cells, tissues, organisms and biomaterials. Areas of application are life science, medicine, agriculture, and environmental science. Similar to the differentiation between "electric" and "electronics" a difference can be made between applications such as Therapy and surgery, which use light mainly to transfer energy, and applications such as diagnostics, which use light to excite matter and to transfer information back to the operator. In most cases the term biophotonics refers to the latter type of application.
https://en.wikipedia.org/wiki/Biophotonics
-------------------------------------------------
Genetically engineered cell becomes world's first living laser
6/3/11
It looks like we're one step closer to having laser vision (or at least, laser-based diagnostic and therapeutic techniques). Researchers at Harvard Medical School and Massachusetts General Hospital in Boston have genetically engineered the world's first "living laser." That's right - a living cell can shoot laser light.
Back in 2008, scientists Martin Chalfie, Roger Tsien, and Osamu Shimomura received the Nobel Prize in chemistry for their roles in bringing Green Fluorescent Protein (also known as GFP, the protein responsible for luminosity in the jellyfish A. victoria) and its diverse applications to the front lines of scientific research.
http://io9.gizmodo.com/5811390/genetically-engineered-cell-becomes-worlds-first-living-laser
----------------------------------------------------
Needle free injection technology: A complete insight
Oct 2015
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4675000/
----------------------------------------------------------
Consider low level laser as treatment option for shingles
ttps://www.chiroeco.com/low-level-laser-treatment-for-shingles/
--------------------------------------------------------
Polymer multilayer tattooing for enhanced DNA vaccination
2013
DNA vaccines have many potential benefits but have failed to generate robust immune responses in humans. Recently, methods such as in vivo electroporation have demonstrated improved performance, but an optimal strategy for safe, reproducible, and pain-free DNA vaccination remains elusive. Here we report an approach for rapid implantation of vaccine-loaded polymer films carrying DNA, immune-stimulatory RNA, and biodegradable polycations into the immune-cell-rich epidermis, using microneedles coated with releasable polyelectrolyte multilayers. Films transferred into the skin following brief microneedle application promoted local transfection and controlled the persistence of DNA and adjuvants in the skin from days to weeks, with kinetics determined by the film composition. These ‘multilayer tattoo’ DNA vaccines induced immune responses against a model HIV antigen comparable to electroporation in mice, enhanced memory T-cell generation, and elicited 140-fold higher gene expression in non-human primate skin than intradermal DNA injection, indicating the potential of this strategy for enhancing DNA vaccination.
http://www.nature.com/nmat/journal/v12/n4/full/nmat3550.html?message-global=remove&foxtrotcallback=true
-------------------------------------------------------
Flu Vaccine Patches Could Be Mailed To Your Home
------------------------------------------------------
----------------
----------------
-----------------
Chapter 9: Big Pharma
-----------------
-----------------
-----------------
Shots In The Dark: Silence on Vaccine
Aug 3, 2011
https://www.youtube.com/watch?v=pnxAsrAK2hw
------------------------------------------------
Are Big Drug Companies Too Slow to Report Patient Deaths to the FDA?
July 2015
A new analysis shows that some pharmaceutical companies may be dragging their feet on disclosing critical data.
A new analysis shows that some pharmaceutical companies may be dragging their feet on disclosing critical data.
https://www.gsb.stanford.edu/insights/are-big-drug-companies-too-slow-report-patient-deaths-fda
------------------------------------------------
New Research Sheds More Light On The Manipulation Of Science By Pharmaceutical Companies
--------------------------------------------------------
Mandatory Meningitis Vaccine Bill Author, Kemp Hannon, Caught Taking $420k From Pharma!
12/02/2015
http://vaxxter.com/mandatory-meningitis-vaccine-bill-author-kemp-hannon-caught-taking-420k-from-pharma/
--------------------------------------
Senator Paid $400,000 By Pharma Pushes Mandatory Vaccine Law
http://vaxxter.com/senator-paid-400000-by-pharma-pushes-mandatory-vaccine-law/
------------------------------------------------------
Hilary Clinton Gets More Big Pharma Money Than Any Other Candidate
2/27/2016
http://vaxxter.com/hilary-clinton-gets-big-pharma-money-candidate/
------------------------------------------------------
Bombshell Study Exposes Frightening Facts About Anti-Depressant Drugs & Pharmaceutical Companies
http://www.collective-evolution.com/2016/02/12/bombshell-study-published-outlining-some-very-frightening-facts-about-anti-depressant-drugs-pharmaceutical-companies/
---------------------------------------------
Ohio Sues 5 Pharma Companies ‘Fueling Opioid Epidemic’
The state of Ohio has sued five major
pharmaceutical companies, essentially claiming they have been ‘fueling’
the state’s ‘opioid epidemic.’
State Attorney General Mike DeWine alleges
these five companies “helped unleash a health care crisis that has had
far-reaching financial, social, and deadly consequences in the State of
Ohio.”
Mississipi is the only other state to have filed a similar lawsuit.
http://vaxxter.com/ohio-sues-5-pharma-companies-fueling-opioid-epidemic/
---------------------------------------------
Papantonio: Daily Death Tolls Caused By Big Pharma’s Opioid Epidemic
According to data by the Center for Disease Control, 91 Americans die every day from overdosing on opioids, in the form of both prescription drugs and drugs like heroin. But its that first class – prescription painkillers – that is a major cause for concern. America’s Lawyer Mike Papantonio talks about the painkiller epidemic brought upon by Big Pharma.
Https://drugsafetynews.com/2017/05/18/papantonio-daily-death-tolls-caused-big-pharmas-opioid-epidemic/
-----------------------------------
Pharma Death clock
Total Deaths in the united states Since January 1, 2000...
Big Pharma's chemical warfare on humanity dwarfs the number of victims killed by all world wars and acts of terrorism combined...
While drug companies profit billions, people are dying by the millions.http://pharmadeathclock.com/
--------------------------------------
How Big Pharma Hides Vaccine Deaths
Adverse reactions can and do include death thanks to additives put in vaccines
July 19, 2017
Vaccine scientists and the public health community cautiously and occasionally will admit that vaccines can cause adverse reactions just like “any other medication or biological product.”
Although experts are less willing to openly disclose the fact that adverse reactions can and do include death, one has only to look at reports to the U.S. Vaccine Adverse Event Reporting System (VAERS) to see that mortality is a possible outcome. From 1990 through 2010, for example, VAERS received 1,881 reports of infant deaths following vaccination, representing 4.8% of the adverse events reported for infants over the 20-year period. Moreover, analysts acknowledge that VAERS, as a passive surveillance system, is subject to substantial underreporting. A federal government report from 2010 affirms that VAERS captures only about 1% of vaccine adverse reports.
https://www.infowars.com/how-big-pharma-hides-vaccine-deaths/
------------------------------------------------
The Elderly Are Being Over Medicated At Scary Rates.
Senior citizens can be horribly taken advantage of in today’s society. That’s not groundbreaking news. But one industry that often flies under the radar for their nefarious activity towards the elderly community is pharma. Opioids, antidepressants, tranquilizers and antipsychotics are all being excessively prescribed by doctors and the combinations of multiple pills often have serious consequences. Pharma is excessively drugging our seniors, so much so, the elderly are developing brain issues; some are even collapsing and experiencing physical injury.
There is a word for this: Polypharmacy. It’s harming the central nervous systems of the elderly. And the medical community is getting away with it rather easily.
http://vaxxter.com/the-elderly-are-being-drugged-at-astounding-scary-rates/
-----------------------------------------------
Nursing Home Pharmacy Settles $9.25 Million For Big Pharma Kickbacks
10/13/2015
http://vaxxter.com/nursing-home-pharmacy-settles-9-25-million-for-big-pharma-kickbacks/
-----------------------------------------------
Vaccine Deaths Not Being Covered by MSM Exposed
Feb 4, 2015
https://www.youtube.com/watch?v=VUzZ9VaZBwI
------------------------------------------
Eustace Mullins - Murder by Injection (Full Length)
Jan 11, 2011
https://www.youtube.com/watch?v=IWkqSncY3fg
-------------------------------------------
The Evils of Big Pharma Exposed
January 2015
Note: Originally published on Global Research in January 2015
What’s wrong with America is what’s wrong with Big Pharma. And what’s wrong with Big Pharma is what’s wrong with America. This circular reality is aimed to be thoroughly covered in this presentation. This is the story of how Big Pharma seeks enormous profits over the health and well-being of the humans it serves, and how drug companies invasively corrupted the way that the healthcare industry delivers its vital services. This is neither a new nor unique story. In fact, the story of Big Pharma is the exact same story of how Big Government, Big Oil, Big Agri-Chem Giants like Monsanto have come to power. The controlling shareholders of all these major industries are one and the same. Big Money belonging to the global central banking cabal own and operate all the Fortune 500 companies in addition to virtually all national governments on this earth. The Rockefellers privatized healthcare in the United States back in the 1930’s and has financed and largely influenced both healthcare and Big Pharma ever since.
The history of the last several centuries is one in which a handful of these oligarch families, primarily from Europe and the United States, have been controlling governments and wars to ruthlessly consolidate and maximize both power and control over the earth’s most precious resources to promote a New World Order of one totalitarian fascist government exercising absolute power and control over the entire global population. This group of oligarch families have systematically and effectively eliminated competition under the deceptive misnomer of a free enterprise system. Modernization is synonymous with globalization, privatization and militarization. Subsequently, an extremely small number of humans representing a privileged ruling elite has imposed a global caste system that’s hatched its long term diabolical plan to actualize its one world government. Sadly at this tumultuous moment in our human history, it’s never been closer to materialization.
http://www.globalresearch.ca/the-evils-of-big-pharma-exposed/5425382
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4 Popular Companies Who Own the Medical Treatments For the Diseases Their Products Cause
------------------------------------------
Big Pharma
https://en.wikipedia.org/wiki/Big_Pharma
----------------------------------------------
Pharmaceutical industry
Pharmaceutical fraud
Pharmaceutical fraud involves deceptions which bring financial gain to a pharmaceutical company. It affects individuals and public and private insurers. There are several different schemes[119] used to defraud the health care system which are particular to the pharmaceutical industry. These include: Good Manufacturing Practice (GMP) Violations, Off Label Marketing, Best Price Fraud, CME Fraud, Medicaid Price Reporting, and Manufactured Compound Drugs.[120] Of this amount $2.5 billion was recovered through False Claims Act cases in FY 2010. Examples of fraud cases include the GlaxoSmithKline $3 billion settlement, Pfizer $2.3 billion settlement and Merck & Co. $650 million settlement. Damages from fraud can be recovered by use of the False Claims Act, most commonly under the qui tam provisions which rewards an individual for being a "whistleblower", or relator (law).[121]
Every major company selling the antipsychotics — Bristol-Myers Squibb, Eli Lilly, Pfizer, AstraZeneca and Johnson & Johnson — has either settled recent government cases, under the False Claims Act, for hundreds of millions of dollars or is currently under investigation for possible health care fraud. Following charges of illegal marketing, two of the settlements set records last year for the largest criminal fines ever imposed on corporations. One involved Eli Lilly's antipsychotic Zyprexa, and the other involved Bextra. In the Bextra case, the government also charged Pfizer with illegally marketing another antipsychotic, Geodon; Pfizer settled that part of the claim for $301 million, without admitting any wrongdoing.[122]
On 2 July 2012, GlaxoSmithKline pleaded guilty to criminal charges and agreed to a $3 billion settlement of the largest health-care fraud case in the U.S. and the largest payment by a drug company.[123] The settlement is related to the company's illegal promotion of prescription drugs, its failure to report safety data,[124] bribing doctors, and promoting medicines for uses for which they were not licensed. The drugs involved were Paxil, Wellbutrin, Advair, Lamictal, and Zofran for off-label, non-covered uses. Those and the drugs Imitrex, Lotronex, Flovent, and Valtrex were involved in the kickback scheme.[125][126][127]
The following is a list of the four largest settlements reached with pharmaceutical companies from 1991 to 2012, rank ordered by the size of the total settlement. Legal claims against the pharmaceutical industry have varied widely over the past two decades, including Medicare and Medicaid fraud, off-label promotion, and inadequate manufacturing practices.[128][129]
| Company | Settlement | Violation(s) | Year | Product(s) | Laws allegedly violated (if applicable) |
|---|---|---|---|---|---|
| GlaxoSmithKline[130] | $3 billion | Off-label promotion/ failure to disclose safety data |
2012 | Avandia/Wellbutrin/Paxil | False Claims Act/FDCA |
| Pfizer[131] | $2.3 billion | Off-label promotion/kickbacks | 2009 | Bextra/Geodon/ Zyvox/Lyrica |
False Claims Act/FDCA |
| Abbott Laboratories[132] | $1.5 billion | Off-label promotion | 2012 | Depakote | False Claims Act/FDCA |
| Eli Lilly[133] | $1.4 billion | Off-label promotion | 2009 | Zyprexa | False Claims Act/FDCA |
https://en.wikipedia.org/wiki/Pharmaceutical_industry
--------------------------------------------
Monsanto
Monsanto Company is a publicly traded American multinational agrochemical and agricultural biotechnology corporation. It is headquartered in Creve Coeur, Greater St. Louis, Missouri. Monsanto is a leading producer of genetically engineered (GE) seed and Roundup, a glyphosate-based herbicide.
Monsanto has agreed to accept Bayer's offer to purchase the company for $66 billion ($128/share) in September 2016, and the deal is currently pending regulatory approval.
Overview
Monsanto has been involved in research on catalytic asymmetric hydrogenation and the first mass-produced light-emitting diodes (LEDs)[5][1] in addition to its work on genetic engineering.
Monsanto was one of four groups to introduce genes into plants (1983),[6] and was among the first to conduct field trials of genetically modified crops, (1987). It was one of the top 10 U.S. chemical companies until it divested most of its chemical businesses between 1997 and 2002, through a process of mergers and spin-offs that focused the company on biotechnology.
Monsanto was one of the first companies to apply the biotechnology industry business model to agriculture, using techniques developed by biotech drug companies.[7]:2–6 In this business model, companies recoup R&D expenses by exploiting biological patents.[8][9][10][11]
Monsanto's roles in agricultural changes, biotechnology products and lobbying of government agencies and roots as a chemical company have surrounded the company in controversies. The company once manufactured controversial products such as the insecticide DDT, PCBs, Agent Orange and recombinant bovine growth hormone. Its seed patenting model was criticized as biopiracy and a threat to biodiversity.
https://en.wikipedia.org/wiki/Monsanto#Current_products
----------------------------------------------
There is No Such Thing as “Free” Vaccines: Why We Rejected Pfizer’s Donation Offer of Pneumonia Vaccines
October 12, 2016
I recently had the difficult task of telling Ian Read, Pfizer’s CEO, that Doctors Without Borders/ Médecins Sans Frontières (MSF) is rejecting the company’s offer to donate a significant number of pneumonia vaccine (PCV) doses for the children we serve. This is not a decision that we took lightly, since our medical teams working in the field witness the impact of pneumonia every day.
Pneumonia claims the lives of nearly one million kids each year, making it the world’s deadliest disease among children. Although there’s a vaccine to prevent this disease, it’s too expensive for many developing countries and humanitarian organizations, such as ours, to afford. As the only producers of the pneumonia vaccine, Pfizer and GlaxoSmithKline (GSK) are able to keep the price of the vaccine artificially high; since 2009, the two companies have earned $36 billion on this vaccine alone. For years, we have been trying to negotiate with the companies to lower the price of the vaccine, but they offered us donations instead.
You might be wondering, then, why we’d rather pay for the vaccine than get it for free. Isn’t free better?
No. Free is not always better. Donations often involve numerous conditions and strings attached, including restrictions on which patient populations and what geographic areas are allowed to receive the benefits. This process can delay starting vaccination campaigns, which would be an untenable situation in emergency settings, or grossly limit who you’re able to reach with the vaccine.Donations can also undermine long-term efforts to increase access to affordable vaccines and medicines. They remove incentives for new manufacturers to enter a market when it’s absorbed through a donation arrangement. We need competition from new companies to bring down prices overall — something we don’t have currently for the pneumonia vaccine.
Donations are often used as a way to make others "pay up." By giving the pneumonia vaccine away for free, pharmaceutical corporations can use this as justification for why prices remain high for others, including other humanitarian organizations and developing countries that also can’t afford the vaccine. Countries, which continue to voice their frustration at being unable to afford new and costly vaccines such as PCV, need lower prices as well to protect children’s health.
Critically, donation offers can disappear as quickly as they come. The donor has ultimate control over when and how they choose to give their products away, risking interruption of programs should the company decide it’s no longer to their advantage. For example, Uganda is now facing a nationwide shortage of Diflucan, an essential crytpococcal meningitis drug, in spite of Pfizer’s commitment to donate the drugs to the government. There are other similar examples of companies’ donation programs leaving governments and health organizations in a lurch without the medical tools they need to treat patients.
To avoid these risks and to limit the use of in-kind medical products donations, the World Health Organization (WHO), and other leading global health organizations such as UNICEF and Gavi, the Vaccine Alliance, have clear recommendations against donation offers from pharmaceutical corporations.
Donations of medical products, such as vaccines and drugs, may appear to be good "quick fixes," but they are not the answer to increasingly high vaccine prices charged by pharmaceutical giants like Pfizer and GSK.
http://www.doctorswithoutborders.org/article/there-no-such-thing-%E2%80%9Cfree%E2%80%9D-vaccines-why-we-rejected-pfizer%E2%80%99s-donation-offer-pneumonia
---------------------------------------------
FRONTLINE | The Vaccine War | PBS
Feb 5, 2015
https://www.youtube.com/watch?v=VPOrnU3ImxI
-------------------------------------------
Is Big Pharma Killing Holistic Doctors?
2016
So what did actually happen with the “five dead doctors?”
The list that’s been going around social media usually consists of the following people, all supposedly holistic doctors or naturopaths, and all dead of “mysterious circumstances” after they’d had encounters with “the feds” – ie, the US government:
• James Jeffrey Bradstreet, an autism researcher and holistic doctor in Georgia, dead of a suicide
• Bruce Hedendal, DC Ph.D, a chiropractor based in Miami, died of natural causes
• Brian Holt, a chiropractor in North Carolina, died of natural causes
• Lisa Riley, an osteopath specializing in emergency medicine based in Georgia, murdered (allegedly) by her husband
• Teresa Sievers, MD, a holistic doctor from Florida, murdered (allegedly) by her husband
Other names sometimes added to the “death list” include:
• Ronald Schwartz, a retired obstetrician licensed in Georgia and Tennessee, shot dead in what looks to be a home invasion
• Amanda Crews, a physician in Modesto, California, shot dead in a murder suicide
• Jeffrey Whiteside, MD, a retired pulmonologist who disappeared in Wisconsin and was found nearly a month later on a vacant lot, dead of a gunshot to the head, with a .22 caliber gun nearby
Among the missing are:
• Patrick Fitzpatrick, MD, a retired ophthalmologist living in North Dakota, who vanished on a walk
• Three doctors who disappeared in Mexico for as-yet unknown reasons
https://skeptoid.com/blog/2015/07/28/is-big-pharma-killing-holistic-doctors/
----------------------------------
Vaccine Excipients
2013 Institute for Vaccine Safety
2‐Phenoxyethanol
3‐O‐desacyl‐4’‐ monophosphoryl lipid A (MPL)
AlbuminAluminum
Amino Acid
Ammonium Sulfate
AmphotericinB
Absorbic AcidBovine Casein
Bovine Casmino acid
Calcium Carbonate
Calcium Chloride
Carbohydrates
Cetyltrimethylammonium Bromide (CTAB)
(CTAB)
Chicken Embryo
Chick Fibroblasts
Chlortetracycline
Dextrose
D-glucose
Dimethyl‐beta‐cyclodextrin
DNA
Egg protein
Egg, Allantoic fluid
Formaldehyde
Galactose
Gelatin
Gelatin Hydrolyzed
Gelatin,Porcine,Hydrolyzed
Glutamate(CTAB)
Chicken Embryo
Chick Fibroblasts
Chlortetracycline
Dextrose
D-glucose
Dimethyl‐beta‐cyclodextrin
DNA
Egg protein
Egg, Allantoic fluid
(EDTA)
Ferric(III) NitrateFormaldehyde
Galactose
Gelatin
Gelatin Hydrolyzed
Gelatin,Porcine,Hydrolyzed
Gentamicin Sulfate
Glutaraldehyde
Glycerin
HEPES
Hexadecyltrimethylammonium Bromide
HydrocortisoneInsect Cell
Kanamycin
Lactalbumin Hydrolysate
Lactose
L‐cystine
L‐histidine
L‐tyrosine
Magnesium Stearate
Magnesium Sulfate
Mannitol
Mouse Serum Protein
MRC-5
Neomycin
Neomycin Sulfate
Nonylphenol Ethoxylate
Octoxynol‐10(Triton X‐100)
Octoxynol‐10 Ethoxylate(Triton X‐100)
Peptone
Peptone,Soy
Phenol
Phenol Red indicator
Polysorbate 80
Porcine DNA
Potassium Aluminum Sulfate
Potassium chloride
Potassium Glutamate
Protamine Sulfate
Salts
Salts and sugars, inorganic
Salts, minerals
Sodium Bicarbonate
Sodium Borate
Sodium Citrate
Sodium Deoxycholate
http://www.drgreenmom.com/wp-content/uploads/2014/04/Vaccine-Excipients-per-0.5mL.pdfL‐tyrosine
Magnesium Stearate
Magnesium Sulfate
Mannitol
Monosodium L-glutamate
Monosodium glutamateMouse Serum Protein
MRC-5
Neomycin
Neomycin Sulfate
Nonylphenol Ethoxylate
Octoxynol‐10(Triton X‐100)
Octoxynol‐10 Ethoxylate(Triton X‐100)
Peptone
Peptone,Soy
Phenol
Phenol Red indicator
Phosphate Buffer
Polymyxin
Polymyxin BPolymyxin
Polysorbate 80
Porcine DNA
Potassium Aluminum Sulfate
Potassium chloride
Potassium Glutamate
Protamine Sulfate
Salts
Salts and sugars, inorganic
Salts, minerals
Sodium Bicarbonate
Sodium Borate
Sodium Citrate
Sodium Deoxycholate
Sodium Metabisulphite
Sodium Pyruvate
Sodium Taurodeoxycholate
Sorbitol
Streptomycin
Thimerosal
Vero Cells
Vitamins
Xanthan
Yeast
Sodium Pyruvate
Sodium Taurodeoxycholate
Sorbitol
Streptomycin
Succinate Buffer
SucroseThimerosal
Vero Cells
Vitamins
Xanthan
Yeast
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Vaccine Excipient & Media Summary
Excipients Included in U.S. Vaccines, by Vaccine
https://www.cdc.gov/vaccines/pubs/pinkbook/downloads/appendices/b/excipient-table-2.pdf
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Vaccines That Contain Potential Allergens
- Vaccine Allergens per 0.5mL (updated 11/14/13)
- Amphotericin B
- Casein (Bovine)
- Chlortetracyclin
- Ovalbumin (Egg)
- Gelatin (Bovine)
- Gelatin (Porcine)
- Gentamicin Sulfate
- Latex
- Neomycin
- Ovalbumin
- Polymyxin B
- Serum Fetal Bovine
- Sodium taurodeoxycholate
- Streptomycin
- Thimerosal
- Yeast
http://www.drgreenmom.com/vaccines/vaccine-ingredients/
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Vaccine Ingredients — A Comprehensive Guide
August 15, 2011
Vaccine Excipient & Media Summary, Part 2 Excipients Included in U.S. Vaccines, by Vaccine.
Includes vaccine ingredients (e.g., adjuvants and preservatives) as well as substances used
during the manufacturing process, including vaccine-production media, that are removed from
the final product and present only in trace quantities. In addition to the substances listed,
most vaccines contain Sodium Chloride (table salt).
Anthrax (BioThrax)
Aluminum Hydroxide, Amino Acids, Benzethonium Chloride, Formaldehyde
or Formalin, Inorganic Salts and Sugars, Vitamins
BCG (Tice)
Asparagine, Citric Acid, Lactose, Glycerin, Iron Ammonium Citrate, Magnesium
Sulfate, Potassium Phosphate
DTaP (Daptacel)
Aluminum Phosphate, Ammonium Sulfate, Casamino Acid,
Dimethyl-beta-cyclodextrin, Formaldehyde or Formalin, Glutaraldehyde,
2-Phenoxyethanol
DTaP (Infanrix)
Aluminum Hydroxide, Bovine Extract, Formaldehyde or Formalin,
Glutaraldhyde, 2-Phenoxyethanol, Polysorbate 80
DTaP (Tripedia)
Aluminum Potassium Sulfate, Ammonium Sulfate, Bovine Extract,
Formaldehyde or Formalin, Gelatin, Polysorbate 80, Sodium Phosphate,
Thimerosal*
DTaP/Hib (TriHIBit)
Aluminum Potassium Sulfate, Ammonium Sulfate, Bovine Extract,
Formaldehyde or Formalin, Gelatin, Polysorbate 80, Sucrose, Thimerosal*
DTaP-IPV (Kinrix)
Aluminum Hydroxide, Bovine Extract, Formaldehyde, Lactalbumin
Hydrolysate, Monkey Kidney Tissue, Neomycin Sulfate, Polymyxin B,
Polysorbate 80
DTaP-HepB-IPV (Pediarix)
Aluminum Hydroxide, Aluminum Phosphate, Bovine Protein, Lactalbumin
Hydrolysate, Formaldehyde or Formalin, Glutaraldhyde, Monkey Kidney Tissue,
Neomycin, 2-Phenoxyethanol, Polymyxin B, Polysorbate 80, Yeast Protein
DtaP-IPV/Hib (Pentacel)
Aluminum Phosphate, Bovine Serum Albumin, Formaldehyde, Glutaraldhyde,
MRC-5 DNA and Cellular Protein, Neomycin, Polymyxin B Sulfate, Polysorbate
80, 2-Phenoxyethanol,
DT (sanofi)
Aluminum Potassium Sulfate, Bovine Extract, Formaldehyde or Formalin,
Thimerosal (multi-dose) or Thimerosal* (single-dose)
DT (Massachusetts)
Aluminum Hydroxide, Formaldehyde or Formalin
Hib (ACTHib)
Ammonium Sulfate, Formaldehyde or Formalin, Sucrose
Hib (Hiberix)
Formaldehyde or Formalin, Lactose
Hib (PedvaxHib)
Aluminum Hydroxyphosphate Sulfate
Hib/Hep B (Comvax)
Amino Acids, Aluminum Hydroxyphosphate Sulfate, Dextrose, Formaldehyde or
Formalin, Mineral Salts, Sodium Borate, Soy Peptone, Yeast Protein
Hep A (Havrix)
Aluminum Hydroxide, Amino Acids, Formaldehyde or Formalin, MRC-5
Cellular Protein, Neomycin Sulfate, 2-Phenoxyethanol, Phosphate Buffers,
Polysorbate
Hep A (Vaqta)
Aluminum Hydroxyphosphate Sulfate, Bovine Albumin or Serum, DNA,
Formaldehyde or Formalin, MRC-5 Cellular Protein, Sodium Borate
Hep B (Engerix-B)
Aluminum Hydroxide, Phosphate Buffers, Thimerosal*, Yeast Protein
Hep B (Recombivax)
Aluminum Hydroxyphosphate Sulfate, Amino Acids, Dextrose, Formaldehyde or
Formalin, Mineral Salts, Potassium Aluminum Sulfate, Soy Peptone, Yeast
Protein
HepA/HepB (Twinrix)
Aluminum Hydroxide, Aluminum Phosphate, Amino Acids, Dextrose,
Formaldehyde or Formalin, Inorganic Salts, MRC-5 Cellular Protein, Neomycin
Sulfate, 2-Phenoxyethanol, Phosphate Buffers, Polysorbate 20, Thimerosal*,
Vitamins, Yeast Protein
Human Papillomavirus (HPV)
(Cerverix)
3-O-desacyl-4’-monophosphoryl lipid A (MPL), Aluminum Hydroxide, Amino
Acids, Insect Cell Protein, Mineral Salts, Sodium Dihydrogen Phosphate
Dihydrate, Vitamins
Human Papillomavirus (HPV)
(Gardasil)
Amino Acids, Amorphous Aluminum Hydroxyphosphate Sulfate,
Carbohydrates, L-histidine, Mineral Salts, Polysorbate 80, Sodium Borate,
Vitamins
Influenza (Afluria)
Beta-Propiolactone, Calcium Chloride, Neomycin, Ovalbumin, Polymyxin B,
Potassium Chloride, Potassium Phosphate, Sodium Phosphate, Sodium
Taurodeoxychoalate
Influenza (Agriflu)
Cetyltrimethylammonium Bromide (CTAB), Egg Protein, Formaldehyde or
Formalin, Kanamycin, Neomycin Sulfate, Polysorbate 80
Influenza (Fluarix)
Egg Albumin (Ovalbumin), Egg Protein, Formaldehyde or Formalin,
Gentamicin, Hydrocortisone, Octoxynol-10,
α-Tocopheryl Hydrogen Succinate,
Polysorbate 80, Sodium Deoxycholate, Sodium Phosphate, Thimerosal*
Influenza (Flulaval)
Egg Albumin (Ovalbumin), Egg Protein, Formaldehyde or Formalin, Sodium
Deoxycholate, Phosphate Buffers, Thimerosal
Influenza (Fluvirin)
Beta-Propiolactone , Egg Protein, Neomycin, Polymyxin B, Polyoxyethylene
9-10 Nonyl Phenol (Triton N-101, Octoxynol 9), Thimerosal (multidose
containers), Thimerosal* (single-dose syringes)
Influenza (Fluzone)
Egg Protein, Formaldehyde or Formalin, Gelatin, Octoxinol-9 (Triton X-100),
Thimerosal (multidose containers)
Influenza (FluMist)
Chick Kidney Cells, Egg Protein, Gentamicin Sulfate, Monosodium Glutamate,
Sucrose Phosphate Glutamate Buffer
IPV (Ipol)
Calf Serum Protein, Formaldehyde or Formalin, Monkey Kidney Tissue,
Neomycin, 2-Phenoxyethanol, Polymyxin B, Streptomycin,
Japanese Encephalitis (JE-Vax)
Formaldehyde or Formalin, Gelatin, Mouse Serum Protein, Polysorbate 80,
Thimerosal
Japanese Encephalitis (Ixiaro)
Aluminum Hydroxide, Bovine Serum Albumin, Formaldehyde, Protamine
Sulfate, Sodium Metabisulphite
Meningococcal (Menactra)
Formaldehyde or Formalin, Phosphate Buffers
Meningococcal (Menomune)
Lactose, Thimerosal (10-dose vials only)
Meningococcal (Menveo)
Amino Acid, Formaldehyde or Formalin, Yeast
MMR (MMR-II)
Amino Acid, Bovine Albumin or Serum, Chick Embryo Fibroblasts, Human
Serum Albumin, Gelatin, Glutamate, Neomycin, Phosphate Buffers, Sorbitol,
Sucrose, Vitamins
MMRV (ProQuad)
Bovine Albumin or Serum, Gelatin, Human Serum Albumin, Monosodium
L-glutamate, MRC-5 Cellular Protein, Neomycin, Sodium Phosphate Dibasic,
Sodium Bicarbonate, Sorbitol, Sucrose, Potassium Phosphate Monobasic,
Potassium Chloride, Potassium Phosphate Dibasic
Pneumococcal (Pneumovax)
Bovine Protein, Phenol
Pneumococcal (Prevnar)
Aluminum Phosphate, Amino Acid, Soy Peptone, Yeast Extract
Pneumococcal (Prevnar 13)
Aluminum Phosphate, Amino Acid, Polysorbate 80, Soy Peptone, Succinate
Buffer, Yeast Extract
Rabies (Imovax)
Human Serum Albumin, Beta-Propiolactone, MRC-5 Cellular Protein,
Neomycin, Phenol Red (Phenolsulfonphthalein), Vitamins
Rabies (RabAvert)
Amphotericin B, Beta-Propiolactone, Bovine Albumin or Serum, Chicken
Protein, Chlortetracycline, Egg Albumin (Ovalbumin),
Ethylenediamine-Tetraacetic Acid Sodium (EDTA), Neomycin, Potassium
Glutamate
Rotavirus (RotaTeq)
Cell Culture Media, Fetal Bovine Serum, Sodium Citrate, Sodium Phosphate
Monobasic Monohydrate, Sodium Hydroxide Sucrose, Polysorbate 80
Rotavirus (Rotarix)
Amino Acids, Calcium Carbonate, Calcium Chloride, D-glucose, Dextran, Ferric
(III) Nitrate, L-cystine, L-tyrosine, Magnesium Sulfate, Phenol Red, Potassium
Chloride, Sodium Hydrogenocarbonate, Sodium Phosphate, Sodium
L-glutamine, Sodium Pyruvate, Sorbitol, Sucrose, Vitamins, Xanthan
Td (Decavac)
Aluminum Potassium Sulfate, Bovine Extract, Formaldehyde or Formalin,
2-Phenoxyethanol, Peptone, Thimerosal*
Td (Massachusetts)
Aluminum Hydroxide, Aluminum Phosphate, Formaldehyde or Formalin,
Thimerosal (some multidose containers)
Tdap (Adacel)
Aluminum Phosphate, Formaldehyde or Formalin, Glutaraldehyde,
2-Phenoxyethanol
Tdap (Boostrix)
Aluminum Hydroxide, Bovine Extract, Formaldehyde or Formalin,
Glutaraldehyde, Polysorbate 80
Typhoid (inactivated – Typhim Vi)
Disodium Phosphate, Monosodium Phosphate, Phenol, Polydimethylsilozone,
Hexadecyltrimethylammonium Bromide
Typhoid (oral – Ty21a)
Amino Acids, Ascorbic Acid, Bovine Protein, Casein, Dextrose, Galactose,
Gelatin, Lactose, Magnesium Stearate, Sucrose, Yeast Extract
Vaccinia (ACAM2000)
Glycerin, Human Serum Albumin, Mannitol, Monkey Kidney Cells, Neomycin,
Phenol, Polymyxin B
Varicella (Varivax)
Bovine Albumin or Serum, Ethylenediamine-Tetraacetic Acid Sodium (EDTA),
Gelatin, Monosodium L-Glutamate, MRC-5 DNA and Cellular Protein,
Neomycin, Potassium Chloride, Potassium Phosphate Monobasic, Sodium
Phosphate Monobasic, Sucrose
Yellow Fever (YF-Vax)
Egg Protein, Gelatin, Sorbitol
Zoster (Zostavax)
Bovine Calf Serum, Hydrolyzed Porcine Gelatin, Monosodium L-glutamate,
MRC-5 DNA and Cellular Protein, Neomycin, Potassium Phosphate Monobasic,
Potassium Chloride, Sodium Phosphate Dibasic, Sucrose
http://vaxtruth.org/2011/08/vaccine-ingredients/------------------------------------------------
Vaccine Ingredients and Vaccine Secrets
http://healthwyze.org/reports/60-vaccine-secrets
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What's Really In Vaccines? Mercury, MSG, Formaldehyde, Aluminum the shocking truth!
Mar 15, 2013
https://www.youtube.com/watch?v=4kiUgJAxAZ0
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18 Little Known Facts About College Meningitis Vaccines
About N. meningitis and the meningitis vaccines
While meningitis sounds frightening and the infection can be serious, here are 18 important, yet little-known, facts about the infection and about the meningitis vaccines you need to know:- In the 10-year period between 1998-2007, only 2,262 cases of meningitis were reported in the United States. Within a population of more than 300 million, that number is negligible and not worth vaccinating millions for “protection.”
- Of the 2,262 cases, 11.3% cases were fatal. Not to minimize the loss of life, but that also means that nearly 89% of people survived and recovered.
- Meningitis does not spread rapidly. According to the Meningitis Research Foundation, the bacterium can only live for a few moments outside the human body, so it is not spread by casual contact and is not passed along on clothing, bedding, toys or dishes.
- In fact, the Foundation goes on to say the risk of the infection is very low and 97 out of 100 cases occur in isolation, with no other cases.
- Neisseria has 12 known bacterial strains, with serogroups A, B, C, W, X, and Y causing almost all infections worldwide. There are five N. meningitidis vaccines approved for use in the U.S.:
- Menomune, for serotypes A, C, Y, W-135, approved in 1981
- Menveo, for serotypes A, C, Y, W-135, approved in 2010
- Menactra, for serotypes A, C, Y, W-135, approved in April 2011
- Trumenba, for serotype B, approved in October 2014
- Bexsero, for serotype B, approved in January 2015
- Before – not after – the introduction of the meningococcal conjugate vaccines, the incidence of meningitis in the United States was already at an historic low.
- Since the introduction of the meningitis vaccines, no significant decrease in serogroup C or Y infection has been seen.
- Menomune, Menactra, and Menveo, recommended for middle school and college, do not generate antibodies against the most common form of the illness, serotype B.
- Menomune still contains mercury.
- Bexsero contains 1,500 mcg of aluminum per dose, the highest amount in any single vaccine.
- There have been no safety studies investigating the injection of Menomune at the same time as other vaccines even though several vaccines, including flu shots or the teen pertussis vaccine, are often given together.
- None of the meningitis vaccines have been tested for carcinogenicity (ability to cause cancer), mutagenicity (ability to disrupt genes) or the ability to interfere with fertility. This holds true for all vaccines.
- Many reports have been filed with the Vaccine Adverse Reporting System (VAERS), documenting vaccine side effects.
- The most recently approved vaccines Trumenba (2014) and Bexsero (2015), have nearly 3,000 adverse events filed with VAERS.
- Menactra has been included in 18,646 VAERS reports.
- Meningitis vaccines may be dangerously disrupting the body’s ability to create its own natural “protection mechanism” against meningitis infection.
15. Serotype B vaccines have not been
manufactured until very recently because the sugar sequences on the
surface of this bacteria are very similar to the sugar sequences on the
surface of human brain and nerve cells. Therefore, vaccine-induced antibodies against serotype B could attack the brain and the nerves, causing a debilitating, life-long, autoimmune reactions.
16. Bexsero was approved soon after an unofficial trial at Stanford University in 2013. Read my full report about the slick maneuver Novartis pulled on American parents to get this vaccine approved.
17. N. meningititis vaccines do not have a long lasting effect. In fact, the CDC admits:
So, Fact #18 points out that increasing the risk from 1
per 100,000 to 3.2 per 100,000 makes it “three times” the risk. This
blatant number twisting happens all the time; one must dig to find the
truth.
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Chapter 10: Nagalase
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Anti-vaccine doctor behind ‘dangerous’ autism therapy found dead. Family cries foul.
June 29, 2015
James Jeffrey Bradstreet’s life was full of controversy. To thousands of supporters, he was a savior: a physician who claimed vaccines caused autism and promoted radical procedures to treat those afflicted, including his own son.
https://www.washingtonpost.com/news/morning-mix/wp/2015/06/29/anti-vaccine-doctor-behind-dangerous-autism-therapy-found-dead-family-cries-foul/
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CDC Knew Its Vaccine Program Was Exposing Children to Dangerous Mercury Levels Since 1999
Uncovered documents show that the U.S. Food and Drug Administration (FDA) and the Centers for Disease Control and Prevention (CDC) knew that infant vaccines were exposing American children to mercury far in excess of all federal safety guidelines since 1999. The documents, created by a FDA consulting toxicologist, show how federal regulators concealed the dangerous impacts and lied to the public.
In 1997, Congress passed the FDA Modernization Act. A provision of that statute required the FDA to "compile a list of drugs that contain intentionally introduced mercury compounds, and provide a quantitative and qualitative analysis of the mercury compounds on the list." In response, manufacturers reported the use of the mercury-based preservative, thimerosal, in more than 30 licensed vaccines.
FDA's Center for Biologics Evaluation and Research (CBER) was responsible for adding up the cumulative exposure to mercury from infant vaccines, a simple calculation that, astonishingly, had never been performed by either the FDA or the CDC. When the agency finally performed that basic calculation, the regulators realized that a six month-old infant who received thimerosal-preserved vaccines following the recommended CDC vaccine schedule would have received a jaw dropping 187.5 micrograms of mercury.
Instead of immediately ordering the removal of thimerosal, FDA officials circled the wagons treating the public health emergency as a public relations problem. Peter Patriarca, then director of the FDA Division of Viral Products, warned his fellow bureaucrats that hasty removal of thimerosal from vaccines would:
" … raise questions about FDA being 'asleep at the switch' for decades
by allowing a potentially hazardous compound to remain in many childhood
vaccines, and not forcing manufacturers to exclude it from new
products. It will also raise questions about various advisory bodies
regarding aggressive recommendations for use. We must keep in mind that
the dose of ethylmercury was not generated by "rocket science."
Conversion of the percentage thimerosal to actual micrograms of mercury
involves ninth grade algebra. What took the FDA so long to do the
calculations? Why didn't CDC and the advisory bodies do these
calculations when they rapidly expanded the childhood immunization
schedule?"
https://www.ecowatch.com/cdc-mercury-vaccines-kennedy-2199157054.html
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Doctors Who Discovered Cancer Enzymes In Vaccines All Found Murdered
Feb 20, 2016
Not long ago, Neon Nettle reported on the epidemic of doctors being murdered, most of which were in Florida, U.S. The scientists all shared a common trait, they had all discovered that nagalase enzyme protein was being added to vaccines which were then administrated to humans.Nagalese is what prevents vitamin D from being produced in the body, which is the body’s main defence to naturally kill cancer cells.
According to Thebigriddle.com: Nagalase is a protein that’s also created by all cancer cells. This protein is also found in very high concentrations in autistic children. And they’re PUTTING it in our vaccines!!
This prevents the body from utilizing the Vitamin D necessary to fight cancer and prevent autism. Nagalese disables the immune system. It’s also known to cause Type 2 Diabetes. So basically…they weren’t killing these doctors because they had found the cure to cancer or were successfully treating autism… they’re killing them because these Dr’s had been researching and had the evidence that the vaccines they’re injecting our precious children with are CAUSING our current cancer and autism crisis!!
And that it’s obviously being done knowingly and on purpose! The Dr’s they killed in FL had been collaborating and were getting ready to go public with the information.
Two doctors, Bradstreet and Gonzalez, were planning on publishing their findings, which centered around nagalase and GcMAF, a definite “smoking gun” if they would have been able to pull the trigger on their alleged findings regarding nagalase in vaccines.
Dr Ted Broer of HealthMasters.com tells the story, as he knows it, in two exceptionally remarkable videos below, which I think everyone who is undecided about vaccines and vaccinations ought to listen to carefully, as Dr Broer explains some of what happens to infants, in the way of reactions after vaccinations, is explained plus why it, “the encephalitic scream,” [1,2] happens biochemically. Pediatricians and family doctors say that’s “normal” and to let baby cry it out! See what you think!
http://cancerremedies.net/doctors-who-discovered-cancer-enzymes-in-vaccines-all-found-murdered/
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Dr. Bradstreet, Nagalase, and the Viral Issue in Autism
In the past months Dr. Bradstreet has become interested in nagalese, which he describes as an enzyme "produced by cancer cells and viruses." He thinks it unlikely that children with autism have undiagnosed cancers, and thus suspicion falls on a viral etiology. Dr. Bradstreet writes, "Viruses make the nagalese enzyme as part of their attachment proteins. It serves to get the virus into the cell and also decreases the body's immune reaction to the virus-thereby increasing the odds of viral survival."
Further on Dr. Bradstreet writes, "It is reasonable and likely that the nature of the immune dysfunction and the frequently observed autoimmune problems in autism are mediated by persistent, unresolved viral infections." He claims to have tested approximately 400 children with autism for the viral marker, nagalese, and found that nearly 80% have significantly elevated levels. He hopes to publish soon on this study and believes this information "is one of the most important developments in the clinical treatment of children on the spectrum that I have experienced in the last 15 years."
Dr. Bradstreet also discusses a substance called GcMAF, which I don't have enough information about to make an informed judgment, and that after viral clearance, the possibility of using neuronal stem cells which can cross the blood-brain barrier. I really can't comment on the advisability of either suggestion.
http://www.ageofautism.com/2011/10/dr-bradstreet-nagalase-and-the-viral-issue-in-autism.html
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VACCINE INGREDIENTS (POISONS)
[These are injected directly into the body, more so in combination. You can see these listed on the Package Inserts, apart from well hidden ones such as Peanut oil and Msg. Poisons deplete nutrients that are crucial defences against infections! Then you have Contaminants like SV40 that is still in the polio vaccines1. Then you may get a badly made batch (see: Vaccine production/manufacturing Vaccination mistakes Vaccine storage), called Hot lots leading to Vaccine Disasters. Then you may get one of their bioweapons, see: Nagalase Vaccine attack. Then the risk rises if your child is ill, they reject sick kids in vaccine trials (see: Healthy trial babies only), then give it to all and sundry including mercury containing flu vaccines for pregnant women 1. Hence the term vaccine roulette, where you take ALL the risk, they get ALL the benefit (see: Why Vaccination Continues.]
VACCINE "CELL LINE" INGREDIENTS:
[2015 pdf] Vaccine contents Included in U.S. Vaccines, by Vaccine this table includes not only vaccine ingredients (e.g., adjuvants and preservatives), but also substances used during the manufacturing process, including vaccine-production media, that are removed from the final product and present only in trace quantities. In addition to the substances listed, most vaccines contain Sodium Chloride (table salt).
[2015 pdf] Vaccine contents Included in U.S. Vaccines, by Vaccine this table includes not only vaccine ingredients (e.g., adjuvants and preservatives), but also substances used during the manufacturing process, including vaccine-production media, that are removed from the final product and present only in trace quantities. In addition to the substances listed, most vaccines contain Sodium Chloride (table salt).
"(Vaccine ingredients) 1. Micro-organisms, either bacteria or
viruses, thought to be causing certain infectious diseases and which the vaccine is
supposed to prevent. These are whole-cell proteins or just the broken-cell protein
envelopes, and are called antigens. 2. Chemical substances
which are supposed to enhance the immune response to the vaccine, called adjuvants. 3. Chemical
substances which act as preservatives and tissue
fixatives, which are supposed to halt any further chemical reactions and
putrefaction (decomposition or multiplication) of the live or attenuated (or killed)
biological constituents of the vaccine."--Viera
Scheibner
http://www.whale.to/vaccine/vaccines/ingredients.html
--------------------------------------------------
Nagalase: Friend and Foe?
What is Nagalase?
Nagalase is a protein made by all cancer cells and viruses (HIV, hepatitis B, hepatitis C, influenza, herpes, Epstein-Barr virus, and others). Its formal, official chemical name is alpha-N-acetylgalactosaminidase, but this is such a tongue-twisting mouthful of a moniker that we usually just call it “Nagalase.” (Sometimes, when I want to impress friends with my brilliance, I’ll say the entire word real fast: “alpha-N-acetylgalactosaminidase.” I have found that it’s important to practice beforehand if one doesn’t want to embarrass oneself.)
Why is Nagalase important?
Nagalase causes immunodeficiency. Nagalase blocks production of GcMAF, thus preventing the immune system from doing its job. Without an active immune system, cancer and viral infections can grow unchecked.
As an extremely sensitive marker for all cancers, Nagalase provides a powerful system for early detection.
Serial Nagalase testing provides a reliable and accurate method for tracking the results of any therapeutic regimen for cancer, AIDS, or other chronic viral infection.
Nagalase proves that cancer cells break all the rules
Normal healthy cells cooperate with one another in a concerted effort to further the good of all. Cancer cells refuse to play ball. Their disdainful attitude toward the rest of our cellular community is appalling. For example, these cellular scofflaws ignore clear messages to stop growing and spreading and encroaching on their neighbor’s space. How would you like it if your neighbor moved his fence over into your backyard?
Of all the rules cancer cells break, none is more alarming than the production of Nagalase, the evil enzyme that completely hog-ties the immune system army’s ability to stop cancer cells.
Virus particles also make Nagalase. Their goal is the same as that of the cancer cells: survival by incapacitating their number one enemy: the immune system.
Nagalase precision
Like a stealth bomber, the Nagalase enzyme synthesized in and released from a cancer cell or a virus particle pinpoints the GcMAF production facilities on the surface of your T and B lymphocytes and then wipes them out with an incredibly precise bomb. How precise? Let me put it this way: Nagalase locates and attacks one specific two-electron bond located at, and only at, the 420th amino acid position on a huge protein molecule (DBP), one of tens of thousands of proteins, each containing millions of electrons. This is like selectively taking out a park bench in a major city from six thousand miles away. More astonishing, if that is possible, Nagalase never misses its target. There is no collateral damage.
As you already know, GcMAF is a cell-signaling glycoprotein that talks to macrophages, enabling them to rapidly find, attack, and kill viruses and cancer cells. By activating macrophages, GcMAF triggers a cascade that activates the entire immune system. Blockage of GcMAF production by Nagalase brings all this wonderful anti-cancer and anti-viral immune activity to a screeching halt, allowing cancer and infections to spread.
Nagalase testing: former mass murderer now works for the good guys
It’s easy to be a little schizy about Nagalase. On the one hand, this nasty protein’s behavior toward us has been reprehensible and disastrous. Working in cahoots with cancer and HIV—not shy about getting into bed with our mortal enemies—Nagalase can rightfully claim direct responsibility for billions of human deaths. And it would just as soon add you to the list, so we don’t have to be shy about placing Nagalase in the “genocidal murderer” column.
With the advent of Nagalase testing, however, this bad actor now will be harnessed to a useful purpose. By providing us with precise and reliable advance information about enemy operations, Nagalase blood level testing becomes a “Deep Throat” double agent for cancer. He helps us by giving us an early warning sign.
http://gcmaf.timsmithmd.com/book/chapter/52
-------------------------------------------
Is This the Real Story of What Happened Regarding Nagalase?
February 25, 2016
During several months of 2015, numerous physicians, who were researching the causes of autism and successfully healing autistics, suddenly met with untimely deaths, or what’s been referred to as “suicided.” Who knows what really happened; all we know is that those physicians no longer are on Planet Earth.
http://www.activistpost.com/2016/02/is-this-the-real-story-of-what-happened-regarding-nagalase.html
--------------------------------------------
GcMAF research (Vaccines)
https://gcmaf.se/
----------------------------------------
GcMAF
Update July 16 2015: GcMAF is no longer available as the company that made it was shutdown by overseas regulatory agencies. As always, consult your doctor before making any medical decisions on any therapy you may be considering.
http://www.betterhealthguy.com/gcmaf
-------------------------------------------
Lyme Disease and GcMAF
Sep 2, 2012
David Noakes talks about the treatment of Lyme Disease with First Immune GcMAF
https://www.youtube.com/watch?v=rjhC6YqIatM
--------------------------------------------
WHO Ranks Antibiotics To Fight Life-Threatening Antibiotic Resistance
WHO say some drug companies will take a financial hit
August 3, 2017https://www.infowars.com/who-ranks-antibiotics-to-fight-life-threatening-antibiotic-resistance/
----------------------------------------
THE NICHOLAS GONZALEZ FOUNDATION
http://www.dr-gonzalez.com/index.htm
-----------------------------------------
---------------
---------------
Chapter 11: Miscellaneous
----------------
----------------
----------------
----------------
China's mass DNA sampling raises concern; Vaccine scandals; Suspicious report claims bumper harvest
Jun 19, 2020
https://www.youtube.com/watch?v=1Op3Qk9O4a4
----------------
Exposing Dangerous Vaccines Declared Hateful and Harassment To Be banned
Mar 28, 2017
https://www.youtube.com/watch?v=sxfFDhyayh8
---------------------------------------------------
CONFLICTING INTERESTS EXPOSED IN MANDATORY VACCINE AGENDA
2015
https://www.youtube.com/watch?v=Ve1_-ri29yU
-------------------------------------------------
The Dangers of Vaccines | New York Foundling Exposed!
Feb 8, 2017
https://www.youtube.com/watch?v=c01i3Ehd0Mw
------------------------------------------------
Shock Video! Gov. Caught Lying About Vaccine Dangers
In this marathon report anchored by a Feb 2014 Senate Hearing on Vaccines featuring Senator and Dr. Anne Schuchat the director of CDC's National Center for Immunization and Respiratory Diseases. They use this national platform to continue the hoax that vaccines are perfectly safe and effective.
https://www.youtube.com/watch?v=k9XRbjOQDvY
---------------------------------------
Vaccine Dangers Exposed Part 1 Of 3
Aug 31, 2010
https://www.youtube.com/watch?v=8j9YY-uAekw
----------------------------------------
Vaccines and Chiropractic: Evidence-Based Medicine or Medical Dogma?
August 15, 2014
http://www.dynamicchiropractic.com/mpacms/dc/article.php?id=57102
------------------------------------------
Vaccine Victims Tell Their Story
Feb 13, 2015Alex Jones and the Infowars crew break down just how dangerous vaccines really are. As the MSM continues an all out assault on freedom, it's our job to speak out and fight back with the truth.
https://www.youtube.com/watch?v=OtlGCidyPtw
----------------------------------------------
WHO Admits Vaccines Caused Polio Paralysis In ISIS Regions
Polio has paralyzed 17 children in the Mayadin and Raqqa regions. Both areas are located in ISIS-controlled regions.
http://vaxxter.com/who-admits-vaccines-caused-polio-paralysis-in-isis-regions/
----------------------------------------
De Niro "Vaccines are dangerous."
Apr 13, 2016
https://www.youtube.com/watch?v=AqFDYQ65AwE
-------------------------------------------------
This Is Exactly Why Jim Carrey Called Out “Government Fascism” In Response To Mandatory Vaccination Law
http://www.collective-evolution.com/2016/06/28/this-is-exactly-why-jim-carrey-called-out-government-fascism-in-response-to-mandatory-vaccination-law/
-------------------------------------------
Newt Gingrich Supports Investigating Vaccines
1/24/2017
http://vaxxter.com/newt-gingrich-supports-investigating-vaccines/
-------------------------------------------
Senator Bernie Sanders: Pharma Gets Away With Murder
1/13/2017
http://vaxxter.com/trump-finds-an-ally-in-bernie-sanders/
--------------------------------------------
Rob Schneider Fired for Exposing Vaccine Risks While Banksters’ New Subprime Racket May Kill You
Sep 26, 2014
https://www.youtube.com/watch?v=gdI8zbV4PvU
---------------------------------------------
Snoop Dogg Exposes Vaccines
Jun 2, 2012
https://www.youtube.com/watch?v=mTU-uXdEq84
-------------------------------------
Gandhi's Anti-Vaccine Views Ring True A Century Later
2015
http://www.greenmedinfo.com/blog/gandhis-anti-vaccine-views-ring-true-century-laterA
------------------------------------------
Emergency Alert: Vaccines are Designed to Kill You
Nov 11, 2015
Alex Jones speaks with long time infowarriors Dr. Rima E. Laibow and Major General Albert Stubblebine III about how you yourself are under genetic attack by the elite and how you need to stand up and fight back.
https://www.youtube.com/watch?v=G2-UxWm-z6k
---------------------------------------
Elite Secret Vaccine Program Exposed
Feb 3, 2015
Alex Jones breaks down how the elites push poison on the masses while keeping the pure vaccines for themselves.
https://www.youtube.com/watch?v=1PNJv518qcQ
----------------------------------------
Dawn of the Bionic Age: Body Hackers let Chips get Under their Skin
‘It can emulate every card in your wallet, so you can chuck your wallet away’
August 4, 2017
https://www.infowars.com/dawn-of-the-bionic-age-body-hackers-let-chips-get-under-their-skin/
----------------------------------------
Scientists Hack a Computer Using DNA
August 10, 2017
Malware can be encoded into a gene and used to take over a computer program.
In what appears to be the first successful hack of a software program using DNA, researchers say malware they incorporated into a genetic molecule allowed them to take control of a computer used to analyze it.
To carry out the hack, researchers led by Tadayoshi Kohno (“see “Innovators Under 35, 2007”) and Luis Ceze encoded malicious software in a short stretch of DNA they purchased online. They then used it to gain “full control” over a computer that tried to process the genetic data after it was read by a DNA sequencing machine.The researchers warn that hackers could one day use faked blood or spit samples to gain access to university computers, steal information from police forensics labs, or infect genome files shared by scientists.
https://www.technologyreview.com/s/608596/scientists-hack-a-computer-using-dna/
--------------------------------------------
Genetically engineered viruses will WIPE OUT hundreds of millions, warns Bill Gates
Feb 20, 2017
BIOTERRORISM has the potential to kill tens of millions of people and is a bigger threat to humanity than nuclear war, Bill Gates has warned.
http://www.express.co.uk/news/science/769636/Bill-Gates-microsoft-bioterrorism-munich-security-conference
----------------------------------------
Vaccine Dangers- Alex Jones Exposes the Truth!!
Feb 4, 2015
https://www.youtube.com/watch?v=wq0Kfz8NJxw
-------------------------------------
Most Dangerous Flu Vaccine Ever Being Pushed On The Public
An important message to anyone considering taking this year’s flu vaccine
Dr. Edward Group | Infowars.com - November 8, 2016
https://www.infowars.com/most-dangerous-flu-vaccine-ever-being-pushed-on-the-public/
-------------------------------------
Dr. Len Horowitz - The Dangers Of Vaccines, Flu Shots & Much More!
Jun 17, 2016
https://www.youtube.com/watch?v=o_WWcncIH6M
----------------------------------------------
Vaxxter
The ultimate guide to vaccine information, anti-pharma news and more
http://vaxxter.com/
----------------------------------------------
Dr Russell Blaylock MD Dangers of Vaccines
May 2014
https://www.youtube.com/watch?v=v5xHV8_Njfc
----------------------------------------
Are Vaccinations Safe for Your Kids? CBN report Dr. Blaylock.
Mar 26, 2013
https://www.youtube.com/watch?v=pp4D7Ui7Aaw
-------------------------------------------
Watch: Racists Caught Putting Poison In Vaccines
Americans must come together to fight against all oppression
August 14, 2017
https://www.infowars.com/watch-racists-caught-putting-poison-in-vaccines/
-------------------------------------------
Black Babies being ‘Disproportionately Affected’ by MMR Vaccine Autism
11/07/2015
http://vaxxter.com/black-babies-being-disproportionately-affected-by-mmr-vaccine-autism/
-----------------------------------------
The Depo Provera shot exposed! Present day eugenics
The Depo Provera has proven side effects that the FDA overlook and target non whites with this product
https://www.youtube.com/watch?v=YDqHtPtTmjI
---------------------------------------
What Bill Nye Gets Wrong About Vaccines & Alternative Medicine
--------------------------------------------------
France To Make 11 Vaccines Mandatory For All Children In 2018
Poll finds only 69 percent of French have confidence in vaccinations
July 5, 2017
https://www.infowars.com/france-to-make-11-vaccines-mandatory-for-all-children-in-2018/
-------------------------------------
California Now Wants to be First State to Mandate Adult Vaccines – Criminal Penalties for those Who Refuse
Will California soon become a medical police state?
July 14, 2015
https://www.infowars.com/california-now-wants-to-be-first-state-to-mandate-adult-vaccines-criminal-penalties-for-those-who-refuse/
-------------------------------------------
California Parents Shut Down Vote on Mandatory Vaccines
Vote postponed after parents clamor for right to refuse vaccinations
April 16, 2015
https://www.infowars.com/california-parents-shut-down-vote-on-mandatory-vaccines/
---------------------------------------
Oklahoma Wants To Force All Kids To Vaccinate
1/23/2017
An Oklahoma State senator named Ervin Yen, who is also a Doctor, is following in the steps of California’s senator, Richard Pan, is now introducing for the third year in a row, mandatory vaccination legislation. The only opt-out provision in Yen’s bill, Senate Bill 83, would be children who a Doctotr signs off on as having a medical issue that would put them at risk by having a vaccine.
http://vaxxter.com/oklahoma-wants-to-force-all-kids-to-vaccinate/
--------------------------------------
Ontario Moves Towards Mandatory Vaccines
12/12/2015
http://vaxxter.com/ontario-moves-towards-mandatory-vaccines/
----------------------------------------
Hundreds Of Illinois Non-Vaccinated Kids Denied School Entry
10/16/2015Peoria, Illinois kids, estimated in the 100s, were denied access to public schools when the schools declared them as not being compliant with vaccines. The main vaccine in question was meningitis. They were said to have not provided documentation to prove they received state mandated vaccines. The children were 6th through 12 grade students.
http://vaxxter.com/hundreds-of-illinois-non-vaccinated-kids-denied-school-entry/
----------------------------------------
BREAKING: 140,000 Anti-Vaccine families cut off from childcare payments
Over 140,000 families have now lost childcare benefits for not complying with vaccination laws in Australia. The situation is looking pretty dire. “No jab, no pay,” is having detrimental effects on families who want parental choice. The Government is essentially rooting them out by starving them of their benefits.
http://vaxxter.com/breaking-140000-anti-vaccine-families-cut-off-from-childcare-payments/
-----------------------------------
Australian Investigating ‘Illegal Vaccine-Free Kindergartens’
10/12/2015
http://vaxxter.com/australian-illegal-vaccine-free-kindergartens-to-be-investigated/
---------------------------------
Vaccines: Penalizing the Unvaccinated?
In a recent article titled, “Endangering the Herd,” Slate argues that those refusing to receive vaccines should be penalized, and the act of refusing to be vaccinated be criminalized
October 15, 2014
https://www.infowars.com/vaccines-penalizing-the-unvaccinated/
---------------------------------------
Victims of Vaccine Damage Can Sue Manufacturers in the US
Will these lawsuits inspire other attorneys and their clients to sue vaccine manufacturers for injury from other adult vaccines?
May 8, 2017
https://www.infowars.com/victims-of-vaccine-damage-can-sue-manufacturers-in-the-us/
----------------------
CONFLICTING INTERESTS EXPOSED IN MANDATORY VACCINE AGENDA
2015
https://www.youtube.com/watch?v=Ve1_-ri29yU
------------------------
What’s Really Behind Mandatory Vaccines?
What they’re not telling you
October 17, 2015
https://www.infowars.com/whats-really-behind-mandatory-vaccines/
--------------------------------------------
You Can Opt Out Of Vaccines In All Fifty States
A concerned caller who doesn’t want to vaccinate her children seeks help
May 17, 2017
https://www.infowars.com/you-can-opt-out-of-vaccines-in-all-fifty-states
--------------------
CDC Vaccine Coverup Exposed
Sep 4, 2014
https://www.youtube.com/watch?v=9ln_-lhXEmY
-------------------
CDC Vaccine Hoaxes Exposed
Feb 20, 2015
https://www.youtube.com/watch?v=cxs_yWlnwJo
------------------------------------------
See How Much ‘Trace Mercury’ & Thimerosal is In Vaccines.
12/16/2015http://vaxxter.com/trace-mercury-thimerosal/
------------------------------------
CDC, VACCINES and AUTISM: Massive Corruption Exposed
Feb 12, 2016
https://www.youtube.com/watch?v=4l3xu7rjol4
--------------------------------------
Triplets Regress into Autism Following Vaccine
4/6/2017
http://vaxxter.com/triplets-regress-into-autism-following-vaccine/
----------------------------------------
Baby Siblings Could Hold Clues to Autism Diagnosis
Studies of infants at risk for the disorder could someday yield a test for it
August 17, 2017
----------------------------------------
Autism-Vaccine Link: Evidence Doesn't Dispel Doubts
http://www.webmd.com/brain/autism/searching-for-answers/vaccines-autism
--------------------
BPA exposure linked to autism spectrum disorder, study reports
March 2, 2015
https://www.sciencedaily.com/releases/2015/03/150302150723.htm
-----------------
Pesticide exposure during pregnancy linked to higher autism risk
June 23, 2014
http://www.latimes.com/science/sciencenow/la-sci-sn-study-finds-link-between-pesticide-exposure-pregnancy-autism-20140623-story.html
--------------
Fine particulate air pollution linked with increased autism risk
December 18, 2014
http://www.hsph.harvard.edu/news/press-releases/fine-particulate-air-pollution-linked-with-increased-autism-risk/
-------------------
The "Western disease": Autism and Somali parents' embodied health movements.
Mar 2017https://www.ncbi.nlm.nih.gov/pubmed/28171816
----------------
Ambient Air Pollution and Autism in Los Angeles County, California
http://ehp.niehs.nih.gov/1205827/
----------------------
MIT Scientist Uncovers Link Between Glyphosate, GMOs And The Autism Epidemic
May 11, 2015
http://reset.me/story/mit-scientist-uncovers-link-between-glyphosate-gmos-and-the-autism-epidemic/
--------------------
Sleep quality influences the cognitive performance of autistic and neurotypical children
May 28th, 2015
https://www.sciencedaily.com/releases/2015/05/150528123855.htm
-----------------------
Brain study reveals insights into genetic basis of autism
July 13th, 2015
https://www.sciencedaily.com/releases/2015/07/150713113336.htm
-------------------
Growing Micro-Brains From Skin Cells Sheds Light On Autism
July 16, 2015
http://www.popsci.com/autism-might-be-result-dampened-brain-signal
--------------------
Researchers unravel a genetic link with autistic behaviors—and find a way to undo it
May 28, 2015
http://www.buffalo.edu/news/releases/2015/05/045.html
-----------------
Full Show - What The MSM Won't Cover - 07/23/2015
https://www.youtube.com/watch?v=k7U-1vtjg3c
----------------------------------
Autism Study: More Evidence Linking Altered Gut Bacteria to ASD
https://www.autismspeaks.org/science/science-news/autism-study-more-evidence-linking-altered-gut-bacteria-asd
-----------------------
Vaccine Herd Immunity Lie Exposed
Feb 16, 2015
https://www.youtube.com/watch?v=3RUeOxvvZfI
----------------------------------
VACCINES: Vietnam Study Confirms Autism Up 33 Times
http://vaxxter.com/vietnam-study-confirms-autism-up-33-times/-----------------------------------
Busted: Gov't Admits Vaccines Cause Autism
( Mar 31, 2016 )
https://www.youtube.com/watch?v=a0glbc0S3sA
------------------------------
Weeding Out Vaccine Toxins: MMR, Glyphosate, and the Health of a Generation
We’ve been misled for far too long by the claim that vaccines are “safe and effective”
August 15, 2017
https://www.infowars.com/weeding-out-vaccine-toxins-mmr-glyphosate-and-the-health-of-a-generation/
-------------------------------
Monsanto’s Glyphosate Found in California Wines, Even Wines Made With Organic Grapes
March 27, 2016
http://ecowatch.com/2016/03/27/monsanto-glyphosate-wine/
----------------
WIN! California to List Glyphosate as a Carcinogen
California will add glyphosate, the main ingredient in Monsanto’s blockbuster herbicide RoundUp, to its list of chemicals known to cause cancer, effective July 7, 2017.
http://naturalsociety.com/win-california-list-glyphosate-carcinogen-1555/
-------------------
Honeybees Are Being Killed off in Europe by 57 Different Pesticides, Study Finds
April 5, 2016
http://www.alternet.org/environment/honeybees-are-being-killed-europe-57-different-pesticides-study-finds
------------------------------
This New Study Found More Drugs in Our Drinking Water Than Anybody Knew
Dec 10, 2013
The new study, which will be released in January in the journal Environmental Pollution, was obtained by The New Republic. Conducted by the Environmental Protection Agency, it is the largest study of water coming out of wastewater treatment plants.
It looked at samples from 50 large-size wastewater treatment plants nationwide and tested for 56 drugs including oxycodone, high-blood pressure medications, and over-the-counter drugs like Tylenol and ibuprofen. More than half the samples tested positive for at least 25 of the drugs monitored, the study said. High blood pressure medications appeared in the highest concentrations and most frequently.
“We were surprised to find that many drugs occurring across all the wastewater plants,” said Mitchell Kostich, the EPA research biologist who led the study. “We were also surprised to see so many drugs of a particular class—the high blood pressure medications—appear at those levels across the board.”
https://newrepublic.com/article/115883/drugs-drinking-water-new-epa-study-finds-more-we-knew
-----------------------------
Toxic tap water? 14 drugs, personal care products in NYC drinking water
Acetaminophen
Butalbital
Caffeine
Carbamazepine
Cotinine
DEET
Diltiazem
Gemfibrozil
Ibuprofen
Iopromide
Meprobamate
Paraxanthine
Primidone
Sulfamethoxazole
https://www.cbsnews.com/pictures/toxic-tap-water-14-drugs-personal-care-products-in-nyc-drinking-water/
----------------------
Unsafe levels of toxic chemicals found in drinking water of 33 states
High levels of fluorinated compounds have been linked to cancer, hormone disruption
Sixty-six of the public water supplies examined, serving six million people, had at least one water sample that measured at or above the EPA safety limit of 70 parts per trillion (ng/L) for two types of PFASs, perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA). Concentrations in some locations ranged as high as 349 ng/L for PFOA and 1,800 ng/L for PFOS.
The highest levels of PFASs were detected in watersheds near industrial sites, military bases, and wastewater treatment plants — all places where these chemicals may be used or found.
http://news.harvard.edu/gazette/story/2016/08/unsafe-levels-of-toxic-chemicals-found-in-drinking-water-of-33-states/
----------------------------------------
Study: High levels of toxic chemicals in drinking water of 6 million Americans
August 9, 2016More than 6 million Americans are drinking water laced with unsafe levels of chemicals linked with cancer and other illnesses, a new report indicates.
Researchers from Harvard T.H. Chan School of Public Health and the Harvard John A. Paulson School of Engineering and Applied Sciences found that levels of a certain class of chemicals -- called polyfluoroalkyl and perfluoroalkyl substances, or PFASs -- are above what's recommended by the federal government in many public drinking water sources.
Study author Xindi Hu told CBS News, "We are interested in this group of compounds -- PFASs -- because they're widely used in a lot of applications."
According to the Environmental Protection Agency, PFASs are used in a broad range of consumer goods, such as cleaners, textiles, leather, paper and paints, fire-fighting foams, and wire insulation.
Food packaging is another source of PFASs, said Hu, a doctoral student in the Department of Environmental Health at Harvard Chan School and Environmental Science and Engineering at SEAS.
She and colleagues compiled and analyzed national data from the EPA, looking at concentrations of six types of PFASs from more than 36,000 drinking water samples collected between 2013-2015.
"We considered major industrial sites that participate in U.S. programs," Hu said. They also identified other sites where PFASs are potentially released to the environment, including military fire training sites, civilian airports where fire-fighting foam containing PFASs is used and wastewater treatment plants.
The researchers reported that 66 of the public water supplies that they studied -- which supplied water to 6 million people -- had at least one water sample that measured at or above the EPA's safety limit of 70 parts per trillion (ng/L) for two types of PFASs: perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA), a chemical commonly used in nonstick cookware.
In some water supplies, the chemical concentrations were found to be significantly higher than the safety limit: close to five times higher than the safety limit for PFOA in Warminster, Pennsylvania, and 25 times higher for PFOS in Newark, Delaware.
In all, the study found that PFASs were detectable at the minimum reporting levels required by the EPA in 194 out of 4,864 water supplies in 33 states across the U.S. Drinking water from 13 states made up 75 percent of those cases. California hd the most instances, followed by New Jersey, North Carolina, Alabama, Florida, Pennsylvania, Ohio, New York, Georgia, Minnesota, Arizona, Massachusetts, and Illinois.
Hu said that the highest levels of PFASs were found in water supplies near industrial sites, military bases and wastewater treatment plants.
https://www.cbsnews.com/news/toxic-chemicals-in-drinking-water-6-million-americans/
----------------------------------
12 Toxins in Your Drinking Water
April 14, 2016
1. Fluoride
2. Chlorine
3. Lead
4. Mercury
5. PCBs
6. Arsenic
7. Perchlorate
8. Dioxins
9. DDT (Dichloro-Diphenyl-Trichloroethane)
10. HCB
11. Dacthal
12. MtBE
https://www.globalhealingcenter.com/natural-health/12-toxins-in-your-drinking-water/
-----------------------------------
Chemical Contaminants in Drinking Water Water
http://inspectapedia.com/water/Chemical_Contaminants_in_Water.phpThe list of chemical contaminant topics in water (below) is of necessity, incomplete, and we continue to add information to this article. Readers are welcome to Contact Us by email with content suggestions or corrections for this article.
- Agricultural sprays, weed killers, etc: in 1962 in Silent Spring, [Book] Rachael Carson drew attention to poisons from insecticides, weed killers, and other common products as well as the use of sprays in agriculture, a practice that led to dangerous chemicals to the food source. Carson argued that those endocrine disrupting chemicals were more dangerous than radiation and that for the first time in history, humans were exposed to chemicals that stayed in their systems from birth to death.
- Endocrine disruptors:
Readers interested in the subtle but powerful effects of hormone
mimicking chemicals and endocrine disruptors that appear in the
environment, their sources, effects, and risks, should also see Our Stolen Future, [Book] Theo Colborn, Dianne Dumanoski, and John Peterson Myers.
Also see our notes on PHTHALETES below.
BPA - Bisphenol-A or BPA, one of the endocrine disruptors under study is also discussed at PLASTIC CONTAINERS, TANKS, TYPES - Iron and other minerals (such as salt) at abnormal levels in water may be a hazard to some individuals. These substances may occur naturally in the water supply. Most comprehensive water tests include a check on the levels of these substances.
- Pesticides and Septic Systems: as we discuss
at CHEMICALS to KEEP OUT OF SEPTICS, people who need to dispose of un-used pesticides should not put them in building drains or toilets. In the U.S. contact your state department of environment or local health department to find the nearest local hazardous waste disposal station where you can usually drop off unwanted chemicals, paint, etc., often at no charge.
See PESTICIDE CONTAMINATION TEST for details of testing for pesticides.
Also see PESTICIDE EXPOSURE HAZARDS for a wider discussion of pesticide exposure hazards in buildings. - "Phthalate Syndrome": Phthalate contamination of groundwater and wells from phthalates: in a compelling article in the New York Times
in July 2009 Nicholas D. Kristof reported on the possible hazards of
phthalates, chemicals that can leach into their contents and thence into
the environment from some plastic food or water containers, or even
toys.
While we have not found any research whatsoever that tests for the appearance of phthalates in septic systems and the ground water into which septic effluent ultimately appears, we recommend prudent avoidance of phthalate containing plastics for foods or beverages. Mr. Kristof pointed out that "These are ubiquitous in modern life ... -- and many scientists have linked them to everything from sexual deformities in babies to obesity and diabetes."
US EPA List of Drinking Water Contaminants
Original Source: http://www.epa.gov/safewater/contaminants/index.htmlThe links in the text that follows will direct the reader to additional details at the US EPA website. You will need to use the "BACK" button on your web browser to return to InspectAPedia.com
Microorganisms in Drinking Water
| Contaminant | MCLG1 (mg/L)2 |
MCL or TT1 (mg/L)2 |
Potential Health Effects from Ingestion of Water | Sources of Contaminant in Drinking Water |
|---|---|---|---|---|
| Cryptosporidium [PDF] |
zero
|
TT 3
|
Gastrointestinal illness (e.g., diarrhea, vomiting, cramps) | Human and animal fecal waste |
| Giardia lamblia |
zero
|
TT3
|
Gastrointestinal illness (e.g., diarrhea, vomiting, cramps) | Human and animal fecal waste |
| Heterotrophic plate count |
n/a
|
TT3
|
HPC has no health effects; it is an analytic method used to measure the variety of bacteria that are common in water. The lower the concentration of bacteria in drinking water, the better maintained the water system is. | HPC measures a range of bacteria that are naturally present in the environment |
| Legionella |
zero
|
TT3
|
Legionnaire's Disease, a type of pneumonia | Found naturally in water; multiplies in heating systems |
| Total Coliforms (including fecal coliform and E. Coli) [Web article] |
zero
|
5.0%4
|
Not a health threat in itself; it is used to indicate whether other potentially harmful bacteria may be present5 | Coliforms are naturally present in the environment; as well as feces; fecal coliforms and E. coli only come from human and animal fecal waste. |
| Turbidity [PDF] |
n/a
|
TT3
|
Turbidity is a measure of the cloudiness of water. It is used to indicate water quality and filtration effectiveness (e.g., whether disease-causing organisms are present). Higher turbidity levels are often associated with higher levels of disease-causing microorganisms such as viruses, parasites and some bacteria. These organisms can cause symptoms such as nausea, cramps, diarrhea, and associated headaches. | Soil runoff |
| Viruses (enteric) |
zero
|
TT3
|
Gastrointestinal illness (e.g., diarrhea, vomiting, cramps) | Human and animal fecal waste |
Disinfection Byproducts Found in Drinking Water
| Contaminant | MCLG1 (mg/L)2 |
MCL or TT1 (mg/L)2 |
Potential Health Effects from Ingestion of Water | Sources of Contaminant in Drinking Water |
|---|---|---|---|---|
| Bromate[PDF] |
zero
|
0.010
|
Increased risk of cancer | Byproduct of drinking water disinfection |
| Chlorite[PDF] |
0.8
|
1.0
|
Anemia; infants & young children: nervous system effects | Byproduct of drinking water disinfection |
| Haloacetic acids (HAA5)[PDF] |
n/a6 [Web]
|
0.0607 [Web]
|
Increased risk of cancer | Byproduct of drinking water disinfection |
| Total Trihalomethanes (TTHMs)[PDF] |
--> n/a6 [Web]
|
--> 0.0807 [Web]
|
Liver, kidney or central nervous system problems; increased risk of cancer | Byproduct of drinking water disinfection |
Disinfectants found in Drinking Water
| Contaminant | MRDLG1 (mg/L)2 |
MRDL1 (mg/L)2 |
Potential Health Effects from Ingestion of Water | Sources of Contaminant in Drinking Water |
|---|---|---|---|---|
| Chloramines (as Cl2) | MRDLG=41 | MRDL=4.01 | Eye/nose irritation; stomach discomfort, anemia | Water additive used to control microbes |
| Chlorine (as Cl2) | MRDLG=41 | MRDL=4.01 | Eye/nose irritation; stomach discomfort | Water additive used to control microbes |
| Chlorine dioxide (as ClO2) | MRDLG=0.81 | MRDL=0.81 | Anemia; infants & young children: nervous system effects | Water additive used to control microbes |
Inorganic Chemicals found in Drinking Water
| Contaminant | MCLG1 (mg/L)2 |
MCL or TT1 (mg/L)2 |
Potential Health Effects from Ingestion of Water | Sources of Contaminant in Drinking Water |
|---|---|---|---|---|
| Antimony |
0.006
|
0.006
|
Increase in blood cholesterol; decrease in blood sugar | Discharge from petroleum refineries; fire retardants; ceramics; electronics; solder |
| Arsenic |
07
|
0.010
as of 01/23/06 |
Skin damage or problems with circulatory systems, and may have increased risk of getting cancer | Erosion of natural deposits; runoff from orchards, runoff from glass & electronicsproduction wastes |
| Asbestos (fiber >10 micrometers) |
7 million fibers per liter
|
7 MFL
|
Increased risk of developing benign intestinal polyps | Decay of asbestos cement in water mains; erosion of natural deposits |
| Barium |
2
|
2
|
Increase in blood pressure | Discharge of drilling wastes; discharge from metal refineries; erosion of natural deposits |
| Beryllium |
0.004
|
0.004
|
Intestinal lesions | Discharge from metal refineries and coal-burning factories; discharge from electrical, aerospace, and defense industries |
| Cadmium |
0.005
|
0.005
|
Kidney damage | Corrosion of galvanized pipes; erosion of natural deposits; discharge from metal refineries; runoff from waste batteries and paints |
| Chromium (total) |
0.1
|
0.1
|
Allergic dermatitis | Discharge from steel and pulp mills; erosion of natural deposits |
| Copper |
1.3
|
TT8;
Action Level=1.3 |
Short term exposure: Gastrointestinal distress Long term exposure: Liver or kidney damage People with Wilson's Disease should consult their personal doctor if the amount of copper in their water exceeds the action level |
Corrosion of household plumbing systems; erosion of natural deposits |
| Cyanide (as free cyanide) |
0.2
|
0.2
|
Nerve damage or thyroid problems | Discharge from steel/metal factories; discharge from plastic and fertilizer factories |
| Fluoride |
4.0
|
4.0
|
Bone disease (pain and tenderness of the bones); Children may get mottled teeth | Water additive which promotes strong teeth; erosion of natural deposits; discharge from fertilizer and aluminum factories |
| Lead |
zero
|
TT8;
Action Level=0.015 |
Infants and children: Delays in physical
or mental development; children could show slight deficits in
attention span and learning abilities Adults: Kidney problems; high blood pressure |
Corrosion of household plumbing systems; erosion of natural deposits |
| Mercury (inorganic) |
0.002
|
0.002
|
Kidney damage | Erosion of natural deposits; discharge from refineries and factories; runoff from landfills and croplands |
| Nitrate (measured as Nitrogen) |
10
|
10
|
Infants below the age of six months who drink water containing nitrate in excess of the MCL could become seriously ill and, if untreated, may die. Symptoms include shortness of breath and blue-baby syndrome. | Runoff from fertilizer use; leaching from septic tanks, sewage; erosion of natural deposits |
| Nitrite (measured as Nitrogen) |
1
|
1
|
Infants below the age of six months who drink water containing nitrite in excess of the MCL could become seriously ill and, if untreated, may die. Symptoms include shortness of breath and blue-baby syndrome. | Runoff from fertilizer use; leaching from septic tanks, sewage; erosion of natural deposits |
| Selenium |
0.05
|
0.05
|
Hair or fingernail loss; numbness in fingers or toes; circulatory problems | Discharge from petroleum refineries; erosion of natural deposits; discharge from mines |
| Thallium |
0.0005
|
0.002
|
Hair loss; changes in blood; kidney, intestine, or liver problems | Leaching from ore-processing sites; discharge from electronics, glass, and drug factories |
Organic Chemicals found in Drinking Water
| Contaminant | MCLG1 (mg/L)2 |
MCL or TT1 (mg/L)2 |
Potential Health Effects from Ingestion of Water
|
Sources of Contaminant in Drinking Water |
|---|---|---|---|---|
| Acrylamide |
zero
|
TT9
|
Nervous system or blood problems; increased risk of cancer | Added to water during sewage/wastewater treatment |
| Alachlor |
zero
|
0.002
|
Eye, liver, kidney or spleen problems; anemia; increased risk of cancer | Runoff from herbicide used on row crops |
| Atrazine |
0.003
|
0.003
|
Cardiovascular system or reproductive problems | Runoff from herbicide used on row crops |
| Benzene |
zero
|
0.005
|
Anemia; decrease in blood platelets; increased risk of cancer | Discharge from factories; leaching from gas storage tanks and landfills |
| Benzo(a)pyrene (PAHs) |
zero
|
0.0002
|
Reproductive difficulties; increased risk of cancer | Leaching from linings of water storage tanks and distribution lines |
| Bisphenol-A, BPA | separate article at InspectApedia.com | What plastic products contain BPA Bisphenol-A and how can you identify them? - separate article, added to the EPA list Also see PLASTIC CONTAINERS, TANKS, TYPES |
||
| Carbofuran |
0.04
|
0.04
|
Problems with blood, nervous system, or reproductive system | Leaching of soil fumigant used on rice and alfalfa |
| Carbon tetrachloride |
zero
|
0.005
|
Liver problems; increased risk of cancer | Discharge from chemical plants and other industrial activities |
| Chlordane |
zero
|
0.002
|
Liver or nervous system problems; increased risk of cancer | Residue of banned termiticide |
| Chlorobenzene |
0.1
|
0.1
|
Liver or kidney problems | Discharge from chemical and agricultural chemical factories |
| 2,4-D |
0.07
|
0.07
|
Kidney, liver, or adrenal gland problems | Runoff from herbicide used on row crops |
| Dalapon |
0.2
|
0.2
|
Minor kidney changes | Runoff from herbicide used on rights of way |
| 1,2-Dibromo-3-chloropropane (DBCP) |
zero
|
0.0002
|
Reproductive difficulties; increased risk of cancer | Runoff/leaching from soil fumigant used on soybeans, cotton, pineapples, and orchards |
| o-Dichlorobenzene |
0.6
|
0.6
|
Liver, kidney, or circulatory system problems | Discharge from industrial chemical factories |
| p-Dichlorobenzene |
0.075
|
0.075
|
Anemia; liver, kidney or spleen damage; changes in blood | Discharge from industrial chemical factories |
| 1,2-Dichloroethane |
zero
|
0.005
|
Increased risk of cancer | Discharge from industrial chemical factories |
| 1,1-Dichloroethylene |
0.007
|
0.007
|
Liver problems | Discharge from industrial chemical factories |
| cis-1,2-Dichloroethylene |
0.07
|
0.07
|
Liver problems | Discharge from industrial chemical factories |
| trans-1,2-Dichloroethylene |
0.1
|
0.1
|
Liver problems | Discharge from industrial chemical factories |
| Dichloromethane |
zero
|
0.005
|
Liver problems; increased risk of cancer | Discharge from drug and chemical factories |
| 1,2-Dichloropropane |
zero
|
0.005
|
Increased risk of cancer | Discharge from industrial chemical factories |
| Di(2-ethylhexyl) adipate |
0.4
|
0.4
|
Weight loss, liver problems, or possible reproductive difficulties. | Discharge from chemical factories |
| Di(2-ethylhexyl) phthalate |
zero
|
0.006
|
Reproductive difficulties; liver problems; increased risk of cancer | Discharge from rubber and chemical factories |
| Dinoseb |
0.007
|
0.007
|
Reproductive difficulties | Runoff from herbicide used on soybeans and vegetables |
| Dioxin (2,3,7,8-TCDD) |
zero
|
0.00000003
|
Reproductive difficulties; increased risk of cancer | Emissions from waste incineration and other combustion; discharge from chemical factories |
| Diquat |
0.02
|
0.02
|
Cataracts | Runoff from herbicide use |
| Endothall |
0.1
|
0.1
|
Stomach and intestinal problems | Runoff from herbicide use |
| Endrin |
0.002
|
0.002
|
Liver problems | Residue of banned insecticide |
| Epichlorohydrin |
zero
|
TT9
|
Increased cancer risk, and over a long period of time, stomach problems | Discharge from industrial chemical factories; an impurity of some water treatment chemicals |
| Ethylbenzene |
0.7
|
0.7
|
Liver or kidneys problems | Discharge from petroleum refineries |
| Ethylene dibromide |
zero
|
0.00005
|
Problems with liver, stomach, reproductive system, or kidneys; increased risk of cancer | Discharge from petroleum refineries |
| Glyphosate |
0.7
|
0.7
|
Kidney problems; reproductive difficulties | Runoff from herbicide use |
| Heptachlor |
zero
|
0.0004
|
Liver damage; increased risk of cancer | Residue of banned termiticide |
| Heptachlor epoxide |
zero
|
0.0002
|
Liver damage; increased risk of cancer | Breakdown of heptachlor |
| Hexachlorobenzene |
zero
|
0.001
|
Liver or kidney problems; reproductive difficulties; increased risk of cancer | Discharge from metal refineries and agricultural chemical factories |
| Hexachlorocyclopentadiene |
0.05
|
0.05
|
Kidney or stomach problems | Discharge from chemical factories |
| Lindane |
0.0002
|
0.0002
|
Liver or kidney problems | Runoff/leaching from insecticide used on cattle, lumber, gardens |
| Methoxychlor |
0.04
|
0.04
|
Reproductive difficulties | Runoff/leaching from insecticide used on fruits, vegetables, alfalfa, livestock |
| Oxamyl (Vydate) |
0.2
|
0.2
|
Slight nervous system effects | Runoff/leaching from insecticide used on apples, potatoes, and tomatoes |
| Polychlorinated biphenyls (PCBs) |
zero
|
0.0005
|
Skin changes; thymus gland problems; immune deficiencies; reproductive or nervous system difficulties; increased risk of cancer | Runoff from landfills; discharge of waste chemicals |
| Pentachlorophenol |
zero
|
0.001
|
Liver or kidney problems; increased cancer risk | Discharge from wood preserving factories |
| Picloram |
0.5
|
0.5
|
Liver problems | Herbicide runoff |
| Simazine |
0.004
|
0.004
|
Problems with blood | Herbicide runoff |
| Styrene |
0.1
|
0.1
|
Liver, kidney, or circulatory system problems | Discharge from rubber and plastic factories; leaching from landfills |
| Tetrachloroethylene |
zero
|
0.005
|
Liver problems; increased risk of cancer | Discharge from factories and dry cleaners |
| Toluene |
1
|
1
|
Nervous system, kidney, or liver problems | Discharge from petroleum factories |
| Toxaphene |
zero
|
0.003
|
Kidney, liver, or thyroid problems; increased risk of cancer | Runoff/leaching from insecticide used on cotton and cattle |
| 2,4,5-TP (Silvex) |
0.05
|
0.05
|
Liver problems | Residue of banned herbicide |
| 1,2,4-Trichlorobenzene |
0.07
|
0.07
|
Changes in adrenal glands | Discharge from textile finishing factories |
| 1,1,1-Trichloroethane |
0.20
|
0.2
|
Liver, nervous system, or circulatory problems | Discharge from metal degreasing sites and other factories |
| 1,1,2-Trichloroethane |
0.003
|
0.005
|
Liver, kidney, or immune system problems | Discharge from industrial chemical factories |
| Trichloroethylene |
zero
|
0.005
|
Liver problems; increased risk of cancer | Discharge from metal degreasing sites and other factories |
| Vinyl chloride |
zero
|
0.002
|
Increased risk of cancer | Leaching from PVC pipes; discharge from plastic factories |
| Xylenes (total) |
10
|
10
|
Nervous system damage | Discharge from petroleum factories; discharge from chemical factories |
Radionuclides found in Drinking Water
| Contaminant | MCLG1 (mg/L)2 |
MCL or TT1 (mg/L)2 |
Potential Health Effects from Ingestion of Water | Sources of Contaminant in Drinking Water |
|---|---|---|---|---|
| Alpha particles |
15 picocuries per Liter (pCi/L)
|
Increased risk of cancer | Erosion of natural deposits of certain minerals that are radioactive and may emit a form of radiation known as alpha radiation | |
| Beta particles and photon emitters |
4 millirems per year
|
Increased risk of cancer | Decay of natural and man-made deposits of certain minerals that are radioactive and may emit forms of radiation known as photons and beta radiation |
|
| Radium 226 and Radium 228 (combined) |
5 pCi/L
|
Increased risk of cancer | Erosion of natural deposits | |
| Uranium |
zero
|
30 ug/L
as of 12/08/03 |
Increased risk of cancer, kidney toxicity | Erosion of natural deposits |
http://inspectapedia.com/water/Chemical_Contaminants_in_Water.php
---------------------------------------
Unregulated Chemicals Found in Drinking Water
Dec 5, 2013
Traces
of 18 unregulated contaminants were found in the water from one-third
of utilities in sampling across the U.S. by federal scientists.
Traces of 18 unregulated chemicals were found in drinking water from more than one-third of U.S. water utilities in a nationwide sampling, according to new, unpublished research by federal scientists.
Included are 11 perfluorinated compounds, an herbicide, two solvents, caffeine, an antibacterial compound, a metal and an antidepressant.
Researchers from the U.S. Geological Survey and the Environmental Protection Agency analyzed single samples of untreated and treated water from 25 U.S. utilities that voluntarily participated in the project.
Twenty-one contaminants were detected – mostly in low concentrations of parts per trillion – in treated drinking water from at least nine of the utilities. Eighteen of the chemicals are not regulated under the federal Safe Drinking Water Act so utilities do not have to meet any limit or even monitor for them.
“The good news is the concentrations are generally pretty low,” said Dana Kolpin, a research hydrologist with the USGS who participated in the study.
“But,” he added, “there’s still the unknown. Are there long-term consequences of low-level exposure to these chemicals?”
For many of the contaminants, little is known about any potential human health effects of low doses. But one of the perfluorinated compounds, known as PFOA, has been linked to a variety of health problems, including cancer, among people in communities where water is contaminated by a chemical plant in West Virginia.
Of 251 chemicals, bacteria, viruses and microbes the scientists measured, 117 were not detected in any of the treated drinking water. Twenty-one were found in water from more than one-third of the 25 utilities (nine or more) and 113 were found in less than one-third (eight or fewer).
EPA research chemist Susan Glassmeyer, who led the project, said the utilities, which remain anonymous, represented a mix of large and small, and used different water treatment technologies.
Preliminary findings of the study, which is expected to be published next year, were presented by the scientists at a toxicology conference in Nashville last month.
While studies increasingly report newly emerging contaminants in wastewater, there has been little data on which ones are in drinking water.
Four of the chemicals found in the samples – the metal strontium, the herbicide metolachlor, PFOS and PFOA – are on the EPA’s list of chemicals under consideration for drinking water standards. The EPA plans to make decisions regarding at least five of the contaminants on its list next year.
https://www.scientificamerican.com/article/unregulated-chemicals-found-in-drinking-water/
------------------------
REPORT: 55 Percent Of New Jersey Schools Have Dangerous Lead In Water
7/18/2017
http://vaxxter.com/55-percent-of-new-jersey-schools-have-dangerous-lead-in-water/
---------------------------------------------------------
Eliminate infections with food-grade hydrogen peroxide
NaturalHealth365) You probably have used hydrogen peroxide many times to treat mild external infections or treat minor cuts. But, ‘food-grade’ hydrogen peroxide actually has a number of additional benefits beyond first aid – although not all uses are recognized by the medical community.
Technically speaking, food grade hydrogen peroxide range from 8 to 35 percent solutions – which shouldn’t be confused with your typical drugstore varieties that get diluted down to about 3.5 percent.
Can food grade hydrogen peroxide help cancer patitents?
One of the most exciting potential uses for hydrogen peroxide, as well as one of the most controversial, involves its use as a cancer therapy. While hydrogen peroxide has not been recognized as a cancer treatment by the mainstream medical profession, there are alternative cancer treatment centers where it is being used.The science behind its use stems from the fact that cancer cells behave in a similar fashion to (most) unwanted pathogens. They prefer consistently acidic environments of low oxygen and plenty of simple sugars. If the biological terrain is rich in oxygen and surrounded by plenty of antioxidants, cancer cells tend to have a poor survival rate.
http://www.naturalhealth365.com/eliminate-infections-hydrogen-peroxide-1113.html/
-----------------------------------------------------------
Hydrogen peroxide fuels aging, inflammation, cancer metabolism and metastasis
2011
Abstract
In 1889, Dr. Stephen Paget proposed the “seed and soil” hypothesis, which states that cancer cells (the seeds) need the proper microenvironment (the soil) for them to grow, spread and metastasize systemically. In this hypothesis, Dr. Paget rightfully recognized that the tumor microenvironment has an important role to play in cancer progression and metastasis. In this regard, a series of recent studies have elegantly shown that the production of hydrogen peroxide, by both cancer cells and cancer-associated fibroblasts, may provide the necessary “fertilizer,” by driving accelerated aging, DNA damage, inflammation and cancer metabolism, in the tumor microenvironment. By secreting hydrogen peroxide, cancer cells and fibroblasts are mimicking the behavior of immune cells (macrophages/neutrophils), driving local and systemic inflammation, via the innate immune response (NFκB). Thus, we should consider using various therapeutic strategies (such as catalase and/or other antioxidants) to neutralize the production of cancer-associated hydrogen peroxide, thereby preventing tumor-stroma co-evolution and metastasis. The implications of these findings for overcoming chemo-resistance in cancer cells are also discussed in the context of hydrogen peroxide production and cancer metabolism.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180186/
-----------------------------------------
Was This Polio Cure Buried For Dangerous Rockefeller Vaccines?
Posted on September 26, 2016
In the 1950s, a cure for polio was squashed by the Rockefellers before the suspicious polio “epidemic” that itself occurred the same decade. In place of the cure, the Rockefellers’ mandatory polio vaccines were presented as the best defense against the disease. These polio vaccines contained SV40 virus which causes cancer tumors.
Obviously, there is enormous financial value in the vaccine industry and a threat of a simple cure for many of these viruses would be a major threat to Big Pharma.
The history of vitamin C is a stunning, and crucial exposure of the medical “industry” and the current vaccine pushers who run the WHO, CDC, AMA, etc.
Two articles bear mentioning when discussing Big Pharma, WHO, AMA, CDC, and the like in the context of mandatory vaccination.
First, “Passover: Suing the True Ange
Essentially, John Hopkins helped develop the Bush pandemic laws (Model State Emergency Health Act) that includes forced vaccination, and pillage of Americans and the country. I highly encourage readers to check out both of these articles to learn more.
I would also recommend that everyone read the article “The Origin Of The 42-Year Stonewall Of Vitamin C,” by Robert Landwehr, published in the Journal of Orthomolecular Medicine.
Vitamin C was stonewalled after Klenner proved it to be a CURE for polio in 1949, before the polio vaccines ever came out. It was Sabin (of Salk and Sabin fame), working for the Rockefellers, who brought out the Salk and Sabin vaccines. Sabin, working for the Rockefellers, may have been involved in repressing the truth about vitamin C.
http://www.naturalblaze.com/2016/09/polio-cure-rockefeller-vaccines.html
-------------------------------------------------------
1947 Rockefeller Patent Shows Origins Of Zika Virus: And What About Those Genetically Modified Mosquitoes?
http://www.collective-evolution.com/2016/02/04/1947-rockefeller-patent-shows-origins-of-zika-virus-and-what-about-those-genetically-modified-mosquitoes/
------------------------------------------------------
Who Owns the Zika Virus?
Global Research 3 February 2016
And who owns the patent on the virus? The Rockefeller Foundation!
http://www.globalresearch.ca/who-owns-the-zika-virus/5505323
-----------------------------------------------------
Zika virus (ATCC® VR-84™)
| Classification | Flaviviridae, Flavivirus |
|---|---|
| Deposited As | Zika |
| Agent | Zika virus |
| Strain | MR 766 (Tissue culture-adapted from ATCC® VR-84™) |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen 1.0 mL |
| Storage Conditions | -70°C or colder |
| Name of Depositor | J. Casals, Rockefeller Foundation |
|---|---|
| Source | Blood from experimental forest sentinel rhesus monkey, Uganda, 1947 |
| Year of Origin | 1947 |
| References | Dick GW, Kitchen SF, and Haddow AJ. Zika Virus. I.
Isolations and Serological Specificity. Trans R Soc Trop Med Hyg 46:
509-520, 1952. PubMed: 12995440
Zhu Z, et al. Comparative genomic analysis of pre-epidemic and epidemic Zika virus strains for virological factors potentially associated with the rapidly expanding epidemic. Emerg Microbes Infect 5: e22, 2016. |
https://www.atcc.org/products/all/VR-84.aspx#history
----------------------------------------------------
Discovery of a Zika antibody offers hope for a vaccine
4-May-2017
A research team based at The Rockefeller University has identified a potent new weapon against the Zika virus in the blood of people who have been infected by it. This discovery could lead to new ways of fighting the disease, including a vaccine.
https://www.eurekalert.org/pub_releases/2017-05/ru-doa050217.php
--------------------------------------------------------
Vaccine Maker Seems To Admit It Needs Zika Outbreak
If you bring up Zika today, you certainly don’t get the emotional discourse that used to occur when Zika was a big media fear mongering deal. Much like Ebola, Zika seems like ancient news, now. But it wasn’t always supposed to end this way. Zika was supposed to be the pharmaceutical industry’s great pandemic hope. They started vaccine productions as the media propped up fears that Zika would overtake the United States. People abandoned trips to Florida, some airlines even compensated potential travelers. The Olympics should have been a death trap.
http://vaxxter.com/vaccine-maker-seems-to-admit-it-needs-zika-outbreak/
------------------------------------------------------
Part II: Rockefeller Vaccine Secret Revealed
The Rockefellers manipulate America’s wealth through the Federal Reserve Bank. Are they manipulating your health through vaccines? You be the judge.
https://vactruth.com/2010/08/18/rockefeller-vaccine-secret-revealed/
----------------------------------------------------------
How the Rockefellers coopted modern medicine and used polio to create vaccine mythology for profit
https://personalliberty.com/rockefellers-coopted-modern-medicine-used-polio-create-vaccine-mythology-profit/
--------------------------------------------------------------
Rockefeller-Funded Anti-Fertility Vaccine Coordinated by WHO
27 August 2010
In addition to the recent PrisonPlanet-exclusive Rockefeller Foundation Developed Vaccines For “Mass-Scale” Fertility Reduction — which outlines the Rockefeller Foundation’s efforts in the 1960s funding research into so-called “anti-fertility vaccines”– another series of documents has surfaced, proving beyond any doubt that the UN Population Fund, World Bank and World Health Organization picked up on it, further developing it under responsibility of a “Task Force on Vaccines for Fertility Regulation”.
http://www.globalresearch.ca/rockefeller-funded-anti-fertility-vaccine-coordinated-by-who/20906
--------------------------------------------------
Kenyan Doctors Used Vaccines To Sterilize Women
10/27/2015
This story is incredibly relevant to our fight, but often times we forget the serious nature of allowing a Government to essentially inject whatever they want into our bodies. Last year, a UN Vaccine Program became the centerpiece of battle over human rights in Kenya. Catholic Bishops discovered evidence came to show that vaccines were used to sterilize women. The charges were disturbing and the people who were effected were shaken to their very cores. It was exacting evidence of our worst fears coming true. It was the reality of a potential slippery slope meeting the surface of our fears.
http://vaxxter.com/kenyan-doctors-used-vaccines-to-sterilize-women/
-------------------------------------------------
----------------------
----------------------
----------------------
Chapter 12: Fetal tissues in Vaccines
------------------------
------------------------
------------------------
Biological safety concepts of genetically modified live bacterial vaccines.
2006
http://europepmc.org/abstract/med/17239999
---------------------------------------------------------
What Aborted Fetuses Have to Do With Vaccines
Feb 17, 2015
A small but growing number of parents who object to vaccinating their children on religious grounds say they do so because many common vaccines are the product of cells that once belonged to aborted fetuses.
There is a grain of truth to this statement. But even religious leaders, including a future pope, have said that shouldn't deter parents from vaccinating their children.
Vaccine and Cell Line Science
Some childhood vaccines, including the one against rubella -- which is part of the MMR vaccine given to millions of children worldwide for measles, mumps and rubella -- is cultured in "WI-38 human diploid lung fibroblasts," according to the U.S. Food and Drug Administration's fact sheet on the vaccine's ingredients.
Merck, the vaccine's manufacturer, acknowledged that those cells were originally obtained from an electively aborted fetus. They were used to start a cell line, which is a cell multiplied over and over again to produce cells that are of a consistent genetic makeup. The WI-38 cell line is used as a culture to grow live viruses that are used in vaccines.
Vaccines Developed Using Human Cell Strains
"Merck, as well as other vaccine manufacturers, uses two well-established human cell lines to grow the virus for selected vaccines," Merck said in a statement to ABC News. "The FDA has approved the use of these cell lines for the production of these Merck vaccines."
Other common vaccines, including those for chicken pox, hepatitis and rabies, are also propagated in cells originating from legally aborted human fetuses, according to the FDA.
"These abortions, which occurred decades ago, were not undertaken with the intent of producing vaccines," said a spokeswoman for the U.S. Centers Disease Control and Prevention.
The original cells were obtained more than 50 years ago and have been maintained under strict federal guidelines by the American Type Culture Collection, according to Merck.
"These cell lines are now more than three generations removed from their origin, and we have not used any new tissue to produce these vaccines," the company added in its statement.
To say that the vaccines contain a significant amount of human fetal tissue, as some objectors to the vaccines claim, is misleading, stressed Dr. Paul Offit, the director of the vaccine education center at the Children's Hospital of Philadelphia.
"There are perhaps nanograms of DNA fragments still found in the vaccine, perhaps billionths of a gram," he said. "You would find as much if you analyzed the fruits and vegetables you eat."
And to remove human fibroblast cells entirely from vaccines is out of the question, Offit explained, noting they are necessary because human viruses don't grow well in animal cells.
"They have also been tested for safety and the fetal cells can go through many more divisions than most other cells before dying," he said...
http://abcnews.go.com/Health/aborted-fetuses-vaccines/story?id=29005539
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Ellen Grills Sandra Bullock On ADREN0CHR0ME Usage - The NPC Show Clips
Jul 9, 2020
https://www.youtube.com/watch?v=qjHzVjkNdcc
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Human Fetal DNA Fragments In Vaccines Are A Possible Cause For Autism – According To This Stanford Scientist
http://www.collective-evolution.com/2014/09/05/human-fetal-dna-fragments-in-vaccines-are-a-possible-cause-for-autism-according-to-this-stanford-scientist/
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Other Scientists claim that human fetal DNA fragments found in vaccines do not cause autism in people.
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Title: Spontaneous Integration of Human DNA Fragments into Host Genome
http://soundchoice.org/wp-content/uploads/2012/08/DNA_Contaminants_in_Vaccines_Can_Integrate_Into_Childrens_Genes.pdf
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Scientists say fetal tissue remains essential for vaccines and developing treatments
August 11, 2015
BOSTON — The furor on Capitol Hill over Planned Parenthood has stoked a debate about the use of tissue from aborted fetuses in medical research, but U.S. scientists have been using such cells for decades to develop vaccines and seek treatments for a host of ailments, from vision loss and neurological disorders to cancer and AIDS.
University laboratories that buy such cells strongly defend their research, saying tissue that would otherwise be thrown out has played a vital role in lifesaving medical advances and holds great potential for further breakthroughs.
Fetal cells are considered ideal because they divide rapidly, adapt to new environments easily and are less susceptible to rejection than adult cells when transplanted.
“If researchers are unable to work with fetal tissue, there is a huge list of diseases for which researchers would move much more slowly, rather than quickly, to find their cause and how they can be cured,” Stanford University spokeswoman Lisa Lapin said in an email.
http://www.pbs.org/newshour/rundown/medical-researchers-say-fetal-tissue-remains-essential/
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What Are MRC-5 and WI-38? And Why Are They in Vaccines?
For the past few years, I have been researching the ins and outs of how our bodies handle toxins and where those toxins come from. When I come across an ingredient I cannot pronounce, a chemical I have never heard of, or an unintelligible acronym, I do what any person (I hope) would do . . . I Google it!
Recently, I came across this list on the CDC’s website that shows the ingredients in vaccines on the market in the United States. These ingredients have been of concern to me after learning about many children who have regressed after vaccination, many of whom also have mitochondrial disease, immune deficiency or other metabolic and immune challenges. It has led me to wonder, is there possibly an ingredient in the vaccine that could cause a child to have a reaction that leads to regression?
Two of the many ingredients listed which I was unfamiliar with seemed like they were written in code, but what caught my eye was these words: “human-diploid fibroblast cell cultures.” Did that mean what I thought it meant? I’d better look this one up!
So I researched them, and here is what I found:
The vaccines that contained these ingredients include:
Adenovirus
DTaP-IPV/Hib (Pentacel)
Hep A (Havrix)
Hep B (Engerix-B)
Hep A/Hep B (Twinrix)
MMR (MMR-II)
MMRV (ProQuad)
Rabies (Imovax)
Varicella (Varivax)
Zoster (Shingles – Zostavax)
MRC-5 (Medical Research Council -5):
“The MRC-5 cell line was developed in September 1966 from lung tissue taken from a 14 week fetus aborted for psychiatric reason from a 27 year old physically healthy woman. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. Cumulative population doublings to senescence is 42-48. G6PD isoenzyme is type B.”
Source: Coriell Institute for Medical Research
And
WI-38 (Wistar Institute-38):
“The WI-38 cell line was developed in July 1962 from lung tissue taken from a therapeutically aborted fetus of about 3 months gestational age. Cells released by trypsin digestion of the lung tissue were used for the primary culture. The cell morphology is fibroblast-like. The karyotype is 46,XX; normal diploid female. A maximum lifespan of 50 population doublings for this culture was obtained at the Repository. A thymidine labelling index of 86% was obtained after recovery. G6PD is isoenzyme type B. This culture of WI-38 is an expansion from passage 9 frozen cells obtained from the submitter.”
Source: Coriell Institute for Medical Research
http://thinkingmomsrevolution.com/mrc-5-wi-38-vaccines/
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WI-38
WI-38 is a diploid human cell culture line composed of fibroblasts derived from lung tissue of an aborted white (caucasian) female fetus.[1] The cell line, isolated by Leonard Hayflick and Paul Moorhead in the 1960s,[2] has been used extensively in scientific research, with applications ranging from developing important theories in molecular biology to the production of many types of vaccines.[3] The contributions from this cell line on research has been credited with saving the lives of "millions of people".[4]
WI-38 is a limited cell line. However, huge amounts of WI-38 cells are already existed. It will take quite a long time to use up the WI-38 cells. One article published in the 1970's suggested that half of the WI-38 cells stocked by Hayflick at the passage 8th would be used up in 66 years from that time.
https://en.wikipedia.org/wiki/WI-38
| ------------------------------------------------------------------ | ||||||||
NA06814
Description:
WI-38 - NORMAL HUMAN FETAL LUNG FIBROBLAST
Affected: No
Gender: Female
Age: 12 FW (At Sampling)
| Repository | NIA Aging Cell Culture Repository | |||
| Subcollection | Specially Characterized Fibroblasts | |||
| Quantity | 0.050mg | |||
| Quantitation Method | Please see our FAQ | |||
| Biopsy Source | Lung | |||
| Cell Type | Fibroblast | |||
| Tissue Type | Lung | |||
| Transformant | Untransformed | |||
| Race | Caucasian | |||
| Family Member | 2 | |||
| Relation to Proband | proband | |||
| Confirmation | Clinical summary/Case history | |||
| ISCN | 46,XX | |||
| Species | Homo sapiens | |||
| Common Name | Human | |||
| Remarks | The WI-38 cell line was developed in July 1962 from lung tissue taken from a therapeutically aborted fetus of about 3 months gestational age. Cells released by trypsin digestion of the lung tissue were used for the primary culture. The cell morphology is fibroblast-like. The karyotype is 46,XX; normal diploid female. A maximum lifespan of 50 population doublings for this culture was obtained at the Repository. A thymidine labelling index of 86% was obtained after recovery. G6PD is isoenzyme type B. This culture of WI-38 is an expansion from passage 9 frozen cells obtained from the submitter. |
https://catalog.coriell.org/0/Sections/Search/Sample_Detail.aspx?Ref=AG06814-N&PgId=166
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AG05965-C Fibroblast from Skin, Lung
Description:
MRC-5 - NORMAL HUMAN FETAL LUNG FIBROBLAST
Affected: No
Gender: Male
Age: 14 FW (At Sampling)
| Repository | NIA Aging Cell Culture Repository |
| Subcollection | Specially Characterized Fibroblasts |
| Biopsy Source | Lung |
| Cell Type | Fibroblast |
| Tissue Type | Lung |
| Transformant | Untransformed |
| Race | Caucasian |
| Relation to Proband | proband |
| Confirmation | Clinical summary/Case history |
| ISCN | 46,XY |
| Species | Homo sapiens |
| Common Name | Human |
| Remarks | The MRC-5 cell line was developed in September 1966 from lung tissue taken from a 14 week fetus aborted for psychiatric reason from a 27 year old physically healthy woman. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. Cumulative population doublings to senescence is 42-48. G6PD isoenzyme is type B. |
https://catalog.coriell.org/0/Sections/Search/Sample_Detail.aspx?Ref=AG05965-C&PgId=166
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The Promise of Adult Stem Cells in Disease Management, Anti-Aging, and Life Extension
Nov 17, 2013
http://articles.mercola.com/sites/articles/archive/2013/11/17/adult-stem-cells-therapy.aspx
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Sickening: Major food corporations use tissue from aborted babies to manufacture flavor additives in processed foods
April 15, 2015
(NaturalNews) Every time you purchase mass-produced processed "food" from the likes of Kraft, PepsiCo, or Nestle, you're choosing, whether you realize it or not, to feed your family not only genetically engineered poisons and chemical additives, but also various flavoring agents manufactured using the tissue of aborted human babies.
http://www.naturalnews.com/049367_aborted_babies_flavor_chemicals_food_corporations.html
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Kraft, Pepsi, and Nestle Using Aborted Babies For Flavor Additives
July 19, 2015
If you thought that Planned Parenthood selling the body parts of aborted babies was evil, then you will be shocked by those companies using these body parts as flavor additives.
The 1973 science fiction film Soylent Green was about food produced from human body parts. It appears Kraft Foods, Pepsi Corporation (PepsiCo) and Nestle are creating a new wave of products using aborted baby body parts provided by Semonyx and StemExpress. Science fiction is now a scientific reality. Should the Food and Drug Administration require a label on those products, listed below, that use baby parts as flavor additives?
http://drrichswier.com/2015/07/19/kraft-pepsi-and-nestle-the-using-aborted-babies-for-flavor-additives/
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Even if you are for or against legalizing abortion in the world,
using human cells for human consumption, especially for flavoring additives, is still a form of cannibalism.
We should not support companies that are using aborted fetus cells, in food additives.
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Famous Food Companies Caught Using Aborted Babies For Flavor Additives
http://conservativepost.com/famous-food-companies-caught-using-aborted-babies-for-flavor-additives/
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The question is, if they are using aborted fetuses for their cells for flavoring additives and vaccines, what is to stop them from trying to produce more fetus cells this way. Does this mean that they will grow fetuses, and harvest fetuses, just for their cells now? To most, this does not sound very ethical with these current accusations.
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Aborted Babies 'Burned to Heat NHS Hospitals'
March 24, 2014
The bodies of at least 15,500 unborn babies have been incinerated over the past two years, with some of them used to generate power to heat NHS hospitals, a new investigation has revealed.
http://www.breitbart.com/london/2014/03/24/aborted-babies-burned-to-heat-nhs-hospitals/
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Sandy Hook Vampires Exposed
April 22, 2017
https://www.youtube.com/watch?v=rUn1jKhWTXI
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Eugenics in Japan
https://en.wikipedia.org/wiki/Eugenics_in_Japan#Abolition_of_eugenics_laws
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Eugenics
https://en.wikipedia.org/wiki/Eugenics
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We can see many of the problems with past vaccines out on the market, we understand that vaccines can be used for many good purposes, including for bad purposes as well. We hope that this information will educate and show the people about the problems with vaccines, for many years to come.
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